Testing for recombinant human erythropoietin in urine: Problems associated with current anti-doping testing

Journal of Applied Physiology

Volumn 105, Issue 2, 2008, Pages 417-419

  Lundby, Carsten ^a   Achman Andersen, Niels J ^a   Thomsen, Jonas J ^a   Norgaard, Anne M ^a   Robach, Paul ^a  

^a International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health   (Denmark)

Author keywords

Detection; Doping; Exercise

Indexed keywords

HEMOGLOBIN; RECOMBINANT ERYTHROPOIETIN; CARBON MONOXIDE; DIAGNOSTIC AGENT;

ACCURACY; ADULT; ARTICLE; ATHLETE; BLOOD ANALYSIS; CONTROLLED STUDY; DOPING; DRUG BLOOD LEVEL; DRUG DETERMINATION; DRUG MISUSE; DRUG URINE LEVEL; EXERCISE TEST; HEMATOCRIT; HUMAN; HUMAN EXPERIMENT; LABORATORY TEST; MALE; NORMAL HUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; URINALYSIS; WADA TEST; LUNG; METABOLISM; URINE;

ADULT; CARBON MONOXIDE; DOPING IN SPORTS; ERYTHROPOIETIN, RECOMBINANT; EXERCISE TEST; HEMATOCRIT; HEMOGLOBINS; HUMANS; LUNG; MALE; OXYGEN CONSUMPTION;

EID: 53449098195     PISSN: 87507587     EISSN: 15221601     Source Type: Journal    
DOI: 10.1152/japplphysiol.90529.2008     Document Type: Article

References (19)

1. Ashenden MJ, Varlet-Marie E, Lasne F, Audran M. The effects of microdose recombinant human erythropoietin regimes in athletes. Haematologica 91: 1143-1144, 2006.
2. Beullens M, Delanghe JR, Bollen M. False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise. Blood 107: 4711-4713, 2006.
3. Burge CM, Skinner SL. Determination of hemoglobin mass and blood volume with CO: evaluation and application of a method. J Appl Physiol 79: 623-631, 1995.
4. Catlin D, Green G, Sekera M, Scott P, Starcevic B. False-positive Epo test concerns unfounded. Blood 108: 1778, 2006.
5. Delanghe JR, Bollen M, Beullens M. Testing for recombinant erythropoietin. Am J Hematol 83: 237-241, 2008.
6. Ekblom B, Berglund B. Effect of erythropoietin administration on maximal aerobic power. Scand J Med Sci Sports 1: 88-93, 1991.
7. Jelkmann W. Erythropoietin: structure, control of production, and function. Physiol Rev 72: 449-489, 1992.
8. Khan A, Grinyer J, Truong ST, Breen EJ, Packer NH. New urinary EPO drug testing method using two-dimensional gel electrophoresis. Clin Chim Acta 358: 119-130, 2005.
9. Lamon S, Robinson N, Sottas PE, Henry H, Kamber M, Mangin P, Saugy M. Possible origins of undetectable EPO in urine samples. Clin Chim Acta 385: 61-66, 2007.
10. Lasne F, de Ceaurriz J. Recombinant erythropoietin in urine. Nature 8: 635, 2000.
11. Lasne F. No doubt about the validity of the urine test for detection of recombinant human erythropoietin. Blood 108: 1778-1779, 2006.
12. Lippi G, Franchini M, Guidi G. Second generation blood tests to detect erythropoietin abuse by athletes: effective but not preventive? Haematologica 89: ELT05, 2004.
13. Lundby C, Hellsten Y, Jensen MBF, Munch AS, Pilegaard H. Erythropoietin receptor in human skeletal muscle and the effects of acute and long-term injections with recombinant human erythropoietin on the skeletal muscle. J Appl Physiol 104: 1154-1160, 2008.
14. Lundby C, Thomsen JJ, Boushel R, Koskolou M, Warberg J, Calbet JAL, Robach P. Erythropoietin treatment elevates haemoglobin concentration by increasing red cell volume and depressing plasma volume. J Physiol 578: 309-314, 2007.
15. Nissen-Lie G, Birkeland K, Hemmersbach P, Skibeli V. Serum sTfR levels may indicate charge profiling of urinary r-hEPO in doping control. Med Sci Sport Exercise 36: 588-593, 2004.
16. Peltre G, Thormann W. Evaluation Report of the Urine Epo Test. Paris, France, and Bern, Switzerland: Council of the World Anti-Doping Agency (WADA), 2003.
17. Thomsen JJ, Rentsch RLRP, Calbet JAL, Boushel R, Juel C, Lundby C. Prolonged administration of recombinant human erythropoietin increases submaximal performance more than maximal aerobic capacity. Eur J Appl Physiol 101: 481-486, 2007.
18. Wide L, Bengtsson C. Molecular charge heterogenity of human serum erythropoietin. Br J Haematol 76: 121-127, 1990.
19. Wide L, Bengtsson C, Berglund B, Ekblom B. Detection in blood and urine of recombinant erythropoietin administered to healthy men. Med Sci Sports Exerc 27: 1569-1576, 1995.