False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise

Blood

Volumn 107, Issue 12, 2006, Pages 4711-4713

  Beullens, Monique ^c   Delanghe, Joris R ^b   Bollen, Mathieu ^a  

^a UNIVERSITY HOSPITALS LEUVEN   (Belgium)

^b GHENT UNIVERSITY HOSPITAL   (Belgium)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN; HORMONE; MONOCLONAL ANTIBODY; RECOMBINANT ERYTHROPOIETIN;

ARTICLE; CASE REPORT; CREATININE CLEARANCE; DOPING; DRUG USE; EXERCISE; FALSE POSITIVE RESULT; FLOW CYTOMETRY; HUMAN; IMMUNOBLOTTING; LABORATORY DIAGNOSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEINURIA; SCREENING; SPORT; URINALYSIS;

EID: 33745075956     PISSN: 00064971     EISSN: 00064971     Source Type: Journal    
DOI: 10.1182/blood-2006-01-0028     Document Type: Article

References (18)

1. Fisher JW. Erythropoietin: physiological and pharmacological aspects. Proc Soc Exp Biol Med. 1997;216:358-369.
2. Cheung JY, Miller BA. Molecular mechanism of erythropoietin signaling. Nephron. 2001;87:215-222.
3. Richmond TD, Chohan M, Barber DL. Turning cells red: signal transduction mediated by erythropoietin. Trends Cell Biology. 2005;15:146-155.
4. Diamanti-Kandarakis E, Konstantinopaulus PA, Papailiou J, Kandarakis SA, Andreopoulos A, Sykiotis GP. Erythropoietin abuse and erythropoietin gene doping. Sports Med. 2005;35:831-840.
5. Sasaki H, Bothner B, Dell A, Fukuda M. Carbohydrate structure of erythropoietin expressed in Chinese hamster ovary cells by human erythropoietin cDNA. J Biol Chem. 1987;262:12059-12076.
6. Skibeli V, Nissen-Lie G, Torjesen P. Sugar profiling proves that human serum erythropoietin differs from recombinant human erythropoietin. Blood. 2001;98:3626-3634.
7. Lasne F, de Ceaurriz J. Recombinant erythropoietin in urine. Nature. 2000;405:635.
8. Catlin DH, Breidbach A, Elliott S, Glaspy J. Comparison of the isoelectric focusing patterns of darbepoetin alfa, recombinant human erythropoietin, and endogenous erythropoietin from human urine. Clin Chem. 2002;48:2057-2059.
9. Lasne F, Martin L, Crepin N, de Ceaurriz J. Detection of isoelectric profiles of erythropoietin in urine: differentiation of natural and administered recombinant hormones. Anal Biochem. 2002;311:119-126.
10. Breidbach A, Catlin DH, Green GA, Trgub I, Trong H, Gorzek J. Detection of recombinant human erythropoietin in urine by isoelectric focusing. Clin Chem. 2003;49:901-907.
11. Lasne F. Double-blotting: a solution to the problem of non-specific binding of secondary antibodies in immunoblotting procedures. J Immunol Methods. 2003;276:223-226.
12. Elliott S, Busse L, Bass MB, et al. Anti-Epo receptor antibodies do not predict Epo receptor expression. Blood. 2006;107:1892-1895.
13. Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254.
14. Fink P, Roemer M, Haeckel R, et al. Measurement of proteins with the Behring Nephelometer: a multicenter evaluation. J Clin Chem Clin Biochem. 1989;27:261-276.
15. Langlois MR, Delanghe JR, Steyaert SR, Everaert KC, De Buyzere ML. Automated flow cytometry compared with an automated dipstick reader for urinalysis. Clin Chem. 1999;45:118-122.
16. Khan A, Grinyer J, Truong ST, Breen EJ, Packer NH. New urinary EPO drug testing method using two-dimensional gel electrophoresis. Clin Chim Acta. 2005;358:119-130.
17. Peltre G, Thormann W. Evaluation report of the urine EPO test. Paris, France, and Bern, Switzerland: Council of the World Anti-Doping Agency (WADA); 2003.
18. Parisotto R, Wu M, Ashenden MJ, et al. Detection of recombinant human erythropoietin abuse in athletes utilizing markers of altered erythropoiesis. Haematologica. 2001;86:128-137.