Detection of Mycoplasma pneumoniae P1 subtype variations by denaturing gradient gel electrophoresis

Diagnostic Microbiology and Infectious Disease

Volumn 78, Issue 1, 2014, Pages 24-28

  Xiao, Jinhong ^a,b   Liu, Yang ^c   Wang, Minggui ^c   Jiang, Chuanhao ^a   You, Xiaoxing ^a   Zhu, Cuiming ^a  

^a UNIVERSITY OF SOUTH CHINA   (China)

^b HUNAN PROVINCIAL PEOPLE S HOSPITAL   (China)

^c FUDAN UNIVERSITY   (China)

Author keywords

P1 gene; Subtpying; Variation

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; BIOTECHNOLOGY; CHINA; CLASSIFICATION; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA SEQUENCE; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC VARIABILITY; INTERMETHOD COMPARISON; MYCOPLASMA PNEUMONIAE; NONHUMAN; P1 GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

P1 GENE; SUBTPYING; VARIATION;

ADHESINS, BACTERIAL; CHINA; DENATURING GRADIENT GEL ELECTROPHORESIS; GENETIC VARIATION; GENOTYPING TECHNIQUES; HUMANS; MYCOPLASMA PNEUMONIAE; POLYMERASE CHAIN REACTION;

EID: 84890118512     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2013.08.008     Document Type: Article

References (23)

1. Atkinson T.P., Balish M.F., Waites K.B. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol Rev 2008, 32:956-973.
2. Cousin-Allery A., Charron A., de Barbeyrac B., Fremy G., Skov Jensen J., Renaudin H., et al. Molecular typing of Mycoplasma pneumoniae strains by PCR-based methods and pulsed-field gel electrophoresis. Application to French and Danish isolates. Epidemiol Infect 2000, 124:103-111.
3. Dégrange S., Cazanave C., Charron A., Renaudin H., Bébéar C., Bébéar C.M. Development of multiple-locus variable-number tandem-repeat analysis for molecular typing of Mycoplasma pneumoniae. J Clin Microbiol 2009, 47:914-923.
4. Dorigo-Zetsma J.W., Wilbrink B., Dankert J., Zaat S.A. Mycoplasma pneumoniae P1 type 1- and type 2-specific sequences within the P1 cytadhesin gene of individual strains. Infect Immun 2001, 69:5612-5618.
5. Dumke R., Jacobs E. Culture-independent multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae. J Microbiol Methods 2011, 86:393-396.
6. Dumke R., Lück P.C., Noppen C., Schaefer C., von Baum H., Marre R., et al. Culture-independent molecular subtyping of Mycoplasma pneumoniae in clinical samples. J Clin Microbiol 2006, 44:2567-2570.
7. Kenri T., Taniguchi R., Sasaki Y., Okazaki N., Narita M., Izumikawa K., et al. Identification of a new variable sequence in the P1 cytadhesin gene of Mycoplasma pneumoniae: evidence for the generation of antigenic variation by DNA recombination between repetitive sequences. Infect Immun 1999, 67:4557-4562.
8. Koo O.K., Mertz A.W., Akins E.L., Sirsat S.A., Neal J.A., Morawicki R., et al. Analysis of microbial diversity on deli slicers using polymerase chain reaction and denaturing gradient gel electrophoresis technologies. Lett Appl Microbiol 2013, 56:111-119.
9. Laguerre G., Allard M.R., Revoy F., Amarger N. Rapid identification of Rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes. Appl Environ Microbiol 1994, 60:56-63.
10. Liu Y., Ye X., Zhang H., Xu X., Li W., Zhu D., et al. Antimicrobial susceptibility of Mycoplasma pneumoniae isolates and molecular analysis of macrolide-resistant strains from Shanghai, China. Antimicrob Agents Chemother 2009, 53:2160-2162.
11. Liu Y., Ye X., Zhang H., Xu X., Li W., Zhu D., et al. Characterization of macrolide resistance in Mycoplasma pneumoniae isolated from children in Shanghai, China. Diagn Microbiol Infect Dis 2010, 67:355-358.
12. Liu Y., Ye X., Zhang H., Xu X., Wang M. Multiclonal origin of macrolide-resistant Mycoplasma pneumoniae isolates as determined by multilocus variable-number tandem-repeat analysis. J Clin Microbiol 2012, 50:2793-2795.
13. Matussek A., Stark L., Dienus O., Aronsson J., Mernelius S., Löfgren S., et al. Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis. J Clin Microbiol 2011, 49:3647-3648.
14. McAuliffe L., Ellis R.J., Ayling R.D., Nicholas R.A. Differentiation of Mycoplasma species by 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis fingerprinting. J Clin Microbiol 2003, 41:4844-4847.
15. Muyzer G. DGGE/TGGE a method for identifying genes from natural ecosystems. Curr Opin Microbiol 1999, 2:317-322.
16. Oates A., Bowling F.L., Boulton A.J., McBain A.J. Molecular and culture-based assessment of the microbial diversity of diabetic chronic foot wounds and contralateral skin sites. J Clin Microbiol 2012, 50:2263-2271.
17. Pereyre S., Charron A., Renaudin H., Bébéar C., Bébéar C.M. First report of macrolide-resistant strains and description of a novel nucleotide sequence variation in the P1 adhesin gene in Mycoplasma pneumoniae clinical strains isolated in France over 12years. J Clin Microbiol 2007, 45:3534-3539.
18. Sambrook J., Russell D.W. Molecular cloning: a laboratory manual. Cold Spring Harbour 2001, Cold Spring Laboratory Press, New York, NY. 3rd edition.
19. Spuesens E.B., Hoogenboezem T., Sluijter M., Hartwig N.G., van Rossum A.M., Vink C. Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae by pyrosequencing. J Microbiol Methods 2010, 82:214-222.
20. Spuesens E.B., Meijer A., Bierschenk D., Hoogenboezem T., Donker G.A., Hartwig N.G., et al. Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae in respiratory specimens collected between 1997 and 2008 in The Netherlands. J Clin Microbiol 2012, 50:1999-2004.
21. Su C., Lei L., Duan Y., Zhang K.Q., Yang J. Culture-independent methods for studying environmental microorganisms: methods, application, and perspective. Appl Microbiol Biotechnol 2012, 93:993-1003.
22. Waites K.B., Talkington D.F. Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev 2004, 17:697-728.
23. Zhao F., Cao B., Li J., Song S., Tao X., Yin Y., et al. Sequence analysis of the p1 adhesin gene of Mycoplasma pneumoniae in clinical isolates collected in Beijing in 2008 to 2009. J Clin Microbiol 2011, 49:3000-3003.