Insights on activity and stability of subtilisin E towards guanidinium chloride and sodium dodecylsulfate

Journal of Biotechnology

Volumn 169, Issue 1, 2014, Pages 87-94

  Li, Zhenwei ^a,b   Roccatano, Danilo ^b   Lorenz, Michael ^c   Martinez, Ronny ^a   Schwaneberg, Ulrich ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b JACOBS UNIVERSITY BREMEN   (Germany)

^c UNIVERSITY OF BREMEN   (Germany)

Author keywords

Chaotolerance; Directed evolution; Guanidinium chloride; Protease; SDS

Indexed keywords

CHAOTOLERANCE; DIRECTED EVOLUTION; GUANIDINIUM CHLORIDES; PROTEASE; SDS;

AMINO ACIDS; DICHROISM; SODIUM DODECYL SULFATE; SUBSTRATES;

CHLORINE COMPOUNDS;

DODECYL SULFATE SODIUM; GUANIDINE; SUBTILISIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; BINDING SITE; CIRCULAR DICHROISM; HALF LIFE TIME; IC 50; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; SITE SATURATION MUTAGENESIS; SPECTROSCOPY;

CHAOTOLERANCE; DIRECTED EVOLUTION; GUANIDINIUM CHLORIDE; PROTEASE; SDS;

BACILLUS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; ENZYME ACTIVATION; ENZYME STABILITY; GUANIDINE; MUTAGENESIS, SITE-DIRECTED; SODIUM DODECYL SULFATE; SUBTILISINS;

EID: 84889562616     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.11.001     Document Type: Article

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