Polarographic Oxygen Sensors, the Oxygraph, and High-Resolution Respirometry to Assess Mitochondrial Function

Drug-Induced Mitochondrial Dysfunction

Volumn , Issue , 2008, Pages 325-352

  Gnaiger, Erich ^a,b  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

^b High Resolution Respirometry   (Austria)

Author keywords

Phosphorylation control protocol (PC protocol); Polarographic oxygen sensor and traditional oxygraphy; Polarographic oxygen sensors (OROBoPOS)

Indexed keywords

EID: 84889491515     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470372531.ch12     Document Type: Chapter

References (66)

1. Dykens JA, Will Y. The significance of mitochondrial toxicity testing in drug development. Drug Discov Today. 2007;12:777-785.
2. Gellerich FN, Mayr JA, Reuter S, Sperl W, Zierz S. The problem of interlab variation in methods for mitochondrial disease diagnosis: enzymatic measurement of respiratory chain complexes. Mitochondrion. 2004;4:427-439.
3. Gnaiger E, Forstner H, eds. Polarographic Oxygen Sensors: Aquatic and Physiological Applications. New York: Springer-Verlag; 1983.
4. Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R. Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr. 1995;27:583-596.
5. Gnaiger E. Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol. 2001;128:277-297.
6. Gnaiger E, Méndez G, Hand SC. High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A. 2000;97:11080-11085.
7. Gnaiger E, Kuznetsov AV. Mitochondrial respiration at low levels of oxygen and cytochrome c. Biochem Soc Trans. 2002;30:252-258.
8. Gnaiger E. Oxygen conformance of cellular respiration: a perspective of mitochondrial physiology. Adv Exp Med Biol. 2003;543:39-56.
9. Pecina P, Gnaiger E, Zeman J, Pronicka E, Hoǔstěk J. Decreased affinity to oxygen of cytochrome c oxidase in Leigh syndrome caused by SURF1 mutations. Am J Physiol Cell Physiol. 2004;287:C1384-C1388.
10. Rostrup M, Fossbakk A, Hauge A, Kleppe R, Gnaiger E, Haavik J. Oxygen dependence of tyrosine hydroxylase. Amino Acids. 2008;34:455-464.
11. Puchowicz MA, Varnes ME, Cohen BH, Frieman NR, Kerr DS, Hoppel CL. Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria: case studies. Mitochondrion. 2004;4:377-385.
12. Gnaiger E, editor. Mitochondrial Pathways and Respiratory Control . Innsbruck, Austria:Oroboros MiPNet Publications; 2007. http://www.oroboros.at.
13. Kuznetsov AV, Schneeberger S, Seiler R, et al. Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol. 2004;286:H1633-H1641.
14. Wenchich L, Drahota1 Z, Honzík T, et al. Polarographic evaluation of mitochondrial enzymes activity in isolated mitochondria and in permeabilized human muscle cells with inherited mitochondrial defects. Physiol Res. 2003;52:781-788.
15. Boushel R, Gnaiger E, Schjerling P, Skovbro M, Kraunsøe R, Dela F. Patients with type 2 diabetes have normal mitochondrial function in skeletal muscle. Diabetologia. 2007;50:790-796.
16. Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E. Senescenceassociated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J. 2004;380:919-928.
17. Hütter E, Unterluggauer H, Garedew A, Jansen-Dürr P, Gnaiger E. High-resolution respirometry: a modern tool in aging research. Exp Gerontol. 2006;41:103-109.
18. Stadlmann S, Rieger G, Amberger A, Kuznetsov AV, Margreiter R, Gnaiger E. H2O2-mediated oxidative stress versus cold ischemia-reperfusion: mitochondrial respiratory defects in cultured human endothelial cells. Transplantation. 2002;74:1800-1803.
19. Aragonés J, Schneider M, Van Geyte K, et al. Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nature Genetics 2008;40:170-180.
20. Renner K, Amberger A, Konwalinka G, Kofler R, Gnaiger E. Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta. 2003;1642:115-123.
21. Hofhaus G, Shakeley RM, Attardi G. Use of polarography to detect respiration defects in cell cultures. Methods Enzymol. 1996;264:476-483.
22. Yamaguchi R, Andreyev A, Murphy AN, Perkins GA, Ellisman MH, Newmeyer DD. Mitochondria frozen with trehalose retain a number of biological functions and preserve outer membrane integrity. Cell Death Differ. 2006;14:616-624.
23. Frezza C, Cipolat S, Scorrano L. Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts. Nat Protocols. 2007;2:287-295.
24. Villani G, Attardi G. Polarographic assays of respiratory chain complex activity. Methods Cell Biol. 2007;80:121-133.
25. Renner K, Kofler R, Gnaiger E. Mitochondrial function in glucocorticoid triggered T-ALL cells with transgenic Bcl-2 expression. Mol. Biol Rep. 2002;29:97-101.
26. Holtzmann D, Moore CL A micro-method for the study of oxidative phosphorylation. Biochim Biophys Acta. 1971;234:1-8.
27. Atkinson HJ, Smith L. An oxygen electrode microrespirometer. J Exp Biol. 1973;59:247-253.
28. Suchy J, Cooper C. Isolation and respiratory measurement on a single large mitochondrion. Exp Cell Res. 1974;88:198-202.
29. Justice RE, Utsunomiya T, Krausz MM, Valeri CR, Shepro D, Hechtman HB. A miniaturized chamber for the measure of oxygen consumption. J Appl Physiol. 1982;52:488-490.
30. Rasmussen HN, Rasmussen UF. Respiration measurements in small scale. Anal Biochem. 1993;208:244-248.
31. Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R. Mitochondria in the cold. In Life in the Cold (G Heldmaier, M Klingenspor, eds). New York: Springer-Verlag; 2000: 431-442.
32. Rasmussen HN, Rasmussen UF. Oxygen solubilities of media used in electrochemical respiration measurements. Anal Biochem. 2003;319:105-113.
33. Baumgärtl H, Lübbers DW. Microaxial needle sensor for polarographic measurement of local O2 pressure in the cellular range of living tissue: its construction and properties. In Polarographic Oxygen Sensors (E Gnaiger, H Forstner, eds). New York:Springer-Verlag; 1983: 37-65.
34. Chance B, Williams GR. Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem. 1955;217:383-393.
35. Lemasters JJ. The ATP-to-oxygen stoichiometries of oxidative phosphorylation by rat liver mitochondria. J Biol Chem. 1984;259:13123-13130.
36. Stoner CD. Determination of the P/2e-stoichiometries at the individual coupling sites in mitochondrial oxidative phosphorylation. J Biol Chem. 1987;262,11445-11453.
37. Robiolio M, Rumsey WL,Wilson DF. Oxygen diffusion and mitochondrial respiration in neuroblastoma cells. Am J Physiol. 1989;256:C1207-C1213.
38. Hollis VS, Palacios-Callender M, Springett RJ, Delpy DT, Moncada S. Monitoring cytochrome redox changes in the mitochondria of intact cells using multi-wavelength visible light spectroscopy. Biochim Biophys Acta. 2003;1607:191-202.
39. Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E. High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS Oxygraph-2k. Proceedings of the 11th Congress of the European Shock Society, Vienna, Austria (H Redl, ed) Bologna, Italy: Medimond International Proceedings; 2005:107-111.
40. Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E. Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol. 1996;271:C2053-C2061.
41. Boveris A, Chance B. The mitochondrial generation of hydrogen peroxide: general properties and effect of hyperbaric oxygen. Biochem J. 1973;134:707-716.
42. Garait B, Couturier K, Servais S, et al. Fat intake reverses the beneficial effects of low caloric intake on skeletal muscle mitochondrial H2O2 production. Free Radic Biol Med. 2005;39:1249-1261.
43. Troppmair J, Rapp UR. Raf and the road to cell survival: a tale of bad spells, ring bearers and detours. Biochem Pharmacol. 2003;66:1341-1345.
44. Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J. Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab. 2001;280:E301-E307.
45. Steinlechner-Maran R, Eberl T, Kunc M, Schröcksnadel H, Margreiter R, Gnaiger E. Respiratory defect as an early event in preservation/reoxygenation injury in endothelial cells. Transplantation. 1997;63:136-142.
46. Stadlmann S, Renner K, Pollheimer J, et al. Preserved coupling of oxydative phosphorylation but decreased mitochondrial respiratory capacity in IL-1β treated human peritoneal mesothelial cells. Cell Biochem Biophys. 2006;44:179-186.
47. Villani G, Attardi G. In vivo control of respiration by cytochrome c oxidase in wildtype and mitochondrial DNA mutation-carrying human cells. Proc Natl Acad Sci U S A. 1997;94:1166-1171.
48. Villani G, Greco M, Papa S, Attardi G. Low reserve capacity of cytochrome c oxidase capacity in vivo in the respiratory chain of a variety of human cell types. J BiolChem. 1998;273:31829-31836.
49. Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E. Evaluation of mitochondrial respiratory function in small biopsies of liver. Anal Biochem. 2002;305:186-194.
50. Mootha VK, Arai AE, Balaban RS. Maximum oxidative phosphorylation capacity of the mammalian heart. Am J Physiol. 1997;272:H769-H775.
51. Saks VA, Veksler VI, Kuznetsov AV, et al. Permeabilised cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem. 1998;184:81-100.
52. Rasmussen UF, Rasmussen HN. Human quadriceps muscle mitochondria: a functional characterization. Mol Cell Biochem. 2000;208:37-44.
53. Gnaiger E, Kuznetsov AV, Lassnig B, et al. High-resolution respirometry. Optimum permeabilization of the cell membrane by digitonin. In BioThermoKinetics in the Post Genomic Era (C Larsson, I-L P°ahlman, L Gustafsson, eds). Göteborg, Sweden:Chalmers Reproservice; 1998: 89-95.
54. Brewer GJ, Jones TT, Wallimann T, Schlattner U. Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants. Mitochondrion. 2004;4:49-57.
55. Gnaiger E, Wright-Paradis C, Sondergaard H, et al. High-resolution respirometry in small biopsies of human muscle: correlations with body mass index and age. Mitochondr Physiol Network. 2005;10(9):14-15. http://www.mitophysiology.org/index.php?gnaigere.
56. Palmer JW, Tandler B, Hoppel CL. Biochemical properties of subsarcolemmal and interfibrillar mitochondria isolated from rat cardiac muscle. J Biol Chem. 1977;252:8731-8739.
57. Riva A, Tandler B, Loffredo F, Vazquez E, Hoppel CL. Structural differences in twobiochemically defined populations of cardiac mitochondria. Am J Physiol Heart Circ Physiol. 2005;289:H868-H872.
58. Sun F, Huo X, Zhai Y, et al. Crystal structure of mitochondrial respiratory membrane protein complex II. Cell. 2005;121:1043-1057.
59. Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E. Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta (EBEC Short Rep Suppl). 2006;14:201-202.
60. Jekabsons MB, Nicholls DG. In situ respiration and bioenergetic status of mitochondria in primary cerebellar granule neuronal cultures exposed continuously to glutamate. J Biol Chem. 2004;279:32989-33000.
61. Brand MD. Measurement of mitochondrial protonmotive force. In Bioenergetics: A Practical Approach (GC Brown, CE Cooper, eds). New York: Oxford University Press; 1995.
62. Labajova A, Vojtiskova A, Krivakova P, Kofranek J, Drahota Z, Houstek J. Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode. Anal Biochem. 2006;353:37-42.
63. Gnaiger E. The twin-flow microrespirometer and simultaneous calorimetry. In Polarographic Oxygen Sensors: Aquatic and Physiological Applications (E Gnaiger, H Forstner, eds). New York: Springer-Verlag; 1983:134-166.
64. Gnaiger E, Kemp RB. Anaerobic metabolism in aerobic mammalian cells: information from the ratio of calorimetric heat flux and respirometric oxygen flux. Biochim Biophys Acta. 1990;1016:328-332.
65. Gnaiger E. Heat dissipation and energetic efficiency in animal anoxibiosis: economy contra power. J Exp Zool. 1983;228:471-490.
66. Aguirre E, Rodriguez-Juarez F, Gnaiger E, Cadenas S. Measurement of the control of cellular respiration by nitric oxide under normoxia and hypoxia: instrumental comparison including high-resolution respirometry. Biochim Biophys Acta. (EBEC Short Rep Suppl). 2006;14:136-137.