Validation of freezing tissues and cells for analysis of DNA strand break levels by comet assay

Mutagenesis

Volumn 28, Issue 6, 2013, Pages 699-707

  Jackson, Petra ^a   Pedersen, Lourdes M ^a   Kyjovska, Zdenka O ^a   Jacobsen, Nicklas R ^a   Saber, Anne T ^a   Hougaard, Karin S ^a   Vogel, Ulla ^a   Wallin, Håkan ^a  

^a NATIONAL RESEARCH CENTRE FOR THE WORKING ENVIRONMENT   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; ARTICLE; BLOOD; COLON; COMET ASSAY; CONTROLLED STUDY; CRYOPRESERVATION; DNA DAMAGE; DNA STRAND BREAKAGE; FEMALE; FREEZE THAWING; LUNG; LUNG ALVEOLUS CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; SAMPLING; STOMACH; TISSUE PREPARATION; TISSUE PRESERVATION; TISSUE SECTION; TRAFFIC AND TRANSPORT; VALIDATION STUDY;

ANIMALS; BRONCHOALVEOLAR LAVAGE FLUID; CELL LINE, TUMOR; COMET ASSAY; CRYOPRESERVATION; DNA BREAKS, DOUBLE-STRANDED; FEMALE; HUMANS; LIVER; LUNG; MICE; MICE, INBRED C57BL;

EID: 84888589544     PISSN: 02678357     EISSN: 14643804     Source Type: Journal    
DOI: 10.1093/mutage/get049     Document Type: Article

References (35)

1. Azqueta, A., Lorenzo, Y. and Collins, A. R. (2009) In vitro comet assay for DNA repair: A warning concerning application to cultured cells. Mutagenesis, 24, 379-381.
2. Azqueta, A., Gutzkow, K. B., Brunborg, G. and Collins, A. R. (2011) Towards a more reliable comet assay: optimising agarose concentration, unwinding time and electrophoresis conditions. Mutat. Res., 724, 41-45.
3. Azqueta, A., Meier, S., Priestley, C., Gutzkow, K. B., Brunborg, G., Sallette, J., Soussaline, F. and Collins, A. (2011) The influence of scoring method on variability in results obtained with the comet assay. Mutagenesis, 26, 393-399.
4. Azqueta, A., Gutzkow, K. B., Priestley, C. C., Meier, S., Walker, J. S., Brunborg, G. and Collins, A. R. (2013) A comparative performance test of standard, medium- And high-throughput comet assays. Toxicol. In Vitro, 27, 768-773.
5. Azqueta, A., Arbillaga, L., López de Cerain, A. and Collins, A. (2013) Enhancing the sensitivity of the comet assay as a genotoxicity test, by combining it with bacterial repair enzyme FPG. Mutagenesis, 28, 271-277.
6. Azqueta, A. and Collins, A. R. (2013) The essential comet assay: A comprehensive guide to measuring DNA damage and repair. Arch. Toxicol., 87, 949-968.
7. Ersson, C. and Möller, L. (2011) The effects on DNA migration of altering parameters in the comet assay protocol such as agarose density, electrophoresis conditions and durations of the enzyme or the alkaline treatments. Mutagenesis, 26, 689-695.
8. Ersson, C., Møller, P., Forchhammer, L., et al. (2013) An ECVAG interlaboratory validation study of the comet assay: inter-laboratory and intra-laboratory variations of DNA strand breaks and FPG-sensitive sites in human mononuclear cells. Mutagenesis, 28, 279-286.
9. Forchhammer, L., Bräuner, E. V., Folkmann, J. K., Danielsen, P. H., Nielsen, C., Jensen, A., Loft, S., Friis, G. and Møller, P. (2008) Variation in assessment of oxidatively damaged DNA in mononuclear blood cells by the comet assay with visual scoring. Mutagenesis, 23, 223-231.
10. Forchhammer, L., Johansson, C., Loft, S., et al. (2010) Variation in the measurement of DNA damage by comet assay measured by the ECVAG inter-laboratory validation trial. Mutagenesis, 25, 113-123.
11. Forchhammer, L., Ersson, C., Loft, S., et al. (2012) Inter-laboratory variation in DNA damage using a standard comet assay protocol. Mutagenesis, 27, 665-672.
12. Johansson, C., Møller, P., Forchhammer, L., et al. (2010) An ECVAG trial on assessment of oxidative damage to DNA measured by the comet assay. Mutagenesis, 25, 125-132.
13. Møller, P., Friis, G., Christensen, P. H., et al. (2004) Intra-laboratory comet assay sample scoring exercise for determination of formamidopyrimidine DNA glycosylase sites in human mononuclear blood cell DNA. Free Radic. Res., 38, 1207-1214.
14. Møller, P. (2006) The alkaline comet assay: Towards validation in biomonitoring of DNA damaging exposures. Basic Clin. Pharmacol. Toxicol., 98, 336-345.
15. Møller, P., Möller, L., Godschalk, R. W. and Jones, G. D. (2010) Assessment and reduction of comet assay variation in relation to DNA damage: studies from the European Comet Assay Validation Group. Mutagenesis, 25, 109-111.
16. Recio, L., Kissling, G. E., Hobbs, C. A. and Witt, K. L. (2012) Comparison of Comet assay dose-response for ethyl methanesulfonate using freshly prepared versus cryopreserved tissues. Environ. Mol. Mutagen., 53, 101-113.
17. Sharma, A. K., Soussaline, F., Sallette, J. and Dybdahl, M. (2012) The influence of the number of cells scored on the sensitivity in the comet assay. Mutat. Res. 749, 70-75.
18. Gutzkow, K. B., Langleite, T. M., Meier, S., Graupner, A., Collins, A. R. and Brunborg, G. (2013) High-throughput comet assay using 96 minigels. Mutagenesis, 28, 333-340.
19. Al-Salmani, K., Abbas, H. H., Schulpen, S., et al. (2011) Simplified method for the collection, storage, and comet assay analysis of DNA damage in whole blood. Free Radic. Biol. Med., 51, 719-725.
20. Bornholdt, J., Dybdahl, M., Vogel, U., Hansen, M., Loft, S. and Wallin, H. (2002) Inhalation of ozone induces DNA strand breaks and inflammation in mice. Mutat. Res. 520, 63-71.
21. Bourdon, J. A., Saber, A. T., Jacobsen, N. R., et al. (2012) Carbon black nanoparticle instillation induces sustained inflammation and genotoxicity in mouse lung and liver. Part. Fibre Toxicol., 9, 5.
22. Dybdahl, M., Risom, L., Møller, P., et al. (2003) DNA adduct formation and oxidative stress in colon and liver of Big Blue rats after dietary exposure to diesel particles. Carcinogenesis, 24, 1759-1766.
23. Dybdahl, M., Risom, L., Bornholdt, J., Autrup, H., Loft, S. and Wallin, H. (2004) Inflammatory and genotoxic effects of diesel particles in vitro and in vivo. Mutat. Res., 562, 119-131.
24. Jackson, P., Hougaard, K. S., Boisen, A. M., et al. (2012) Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: effects on liver DNA strand breaks in dams and offspring. Nanotoxicology, 6, 486-500.
25. Jackson, P., Halappanavar, S., Hougaard, K. S., et al. (2013) Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression. Nanotoxicology, 7, 85-96.
26. Jacobsen, N. R., Møller, P., Jensen, K. A., Vogel, U., Ladefoged, O., Loft, S. and Wallin, H. (2009) Lung inflammation and genotoxicity following pulmonary exposure to nanoparticles in ApoE-/- mice. Part. Fibre Toxicol., 6, 2.
27. Madsen, A. M., Saber, A. T., Nordly, P., Sharma, A. K., Wallin, H. and Vogel, U. (2008) Inflammation but no DNA (deoxyribonucleic acid) damage in mice exposed to airborne dust from a biofuel plant. Scand. J. Work. Environ. Health, 34, 278-277.
28. Møller, P., Wallin, H., Vogel, U., et al. (2002) Mutagenicity of 2-amino- 3-methylimidazo[4, 5-f]quinoline in colon and liver of Big Blue rats: role of DNA adducts, strand breaks, DNA repair and oxidative stress. Carcinogenesis, 23, 1379-1385.
29. Saber, A. T., Jensen, K. A., Jacobsen, N. R., Birkedal, R., Mikkelsen, L., Møller, P., Loft, S., Wallin, H. and Vogel, U. (2012) Inflammatory and genotoxic effects of nanoparticles designed for inclusion in paints and lacquers. Nanotoxicology, 6, 453-471.
30. Saber, A. T., Koponen, I. K., Jensen, K. A., Jacobsen, N. R., Mikkelsen, L., Møller, P., Loft, S., Vogel, U. and Wallin, H. (2012) Inflammatory and genotoxic effects of sanding dust generated from nanoparticle-containing paints and lacquers. Nanotoxicology, 6, 776-788.
31. Saber, A. T., Jacobsen, N. R., Mortensen, A., et al. (2012) Nanotitanium dioxide toxicity in mouse lung is reduced in sanding dust from paint. Part. Fibre Toxicol., 9, 4.
32. Danielsen, P. H., Risom, L., Wallin, H., Autrup, H., Vogel, U., Loft, S. and Møller, P. (2008) DNA damage in rats after a single oral exposure to diesel exhaust particles. Mutat. Res., 637, 49-55.
33. Jackson, P., Lund, S. P., Kristiansen, G., Andersen, O., Vogel, U., Wallin, H. and Hougaard, K. S. (2011) An experimental protocol for maternal pulmonary exposure in developmental toxicology. Basic Clin. Pharmacol. Toxicol., 108, 202-207.
34. Hougaard, K. S., Jackson, P., Jensen, K. A., et al. (2010) Effects of prenatal exposure to surface-coated nanosized titanium dioxide (UV-Titan). A study in mice. Part. Fibre Toxicol., 7, 16.
35. Brunborg, G., Søderlund, E. J., Holme, J. A. and Dybing, E. (1996) Organspecific and transplacental DNA damage and its repair in rats treated with 1, 2-dibromo-3-chloropropane. Chem. Biol. Interact., 101, 33-48.