Enhancing the sensitivity of the comet assay as a genotoxicity test, by combining it with bacterial repair enzyme FPG

Mutagenesis

Volumn 28, Issue 3, 2013, Pages 271-277

  Azqueta, Amaya ^a,b   Arbillaga, Leire ^a   López De Cerain, Adela ^a   Collins, Andrew ^b  

^a UNIVERSITY OF NAVARRA   (Spain)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

4 NITROQUINOLINE 1 OXIDE; BENZO[A]PYRENE; CYCLOPHOSPHAMIDE; DNA BASE; DNA FORMAMIDOPYRIMIDINE GLYCOSYLASE; EDETIC ACID; ETOPOSIDE; FLUOMETURON; MANNITOL; MESYLIC ACID DERIVATIVE; METHYLMETHANESULPHONATE; METHYLNITROSOUREA; TRITON X 100; TROMETAMOL; UNCLASSIFIED DRUG;

ANALYTICAL EQUIPMENT; ARTICLE; CELL PROLIFERATION; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DNA REPAIR; DNA STRAND BREAKAGE; ENZYME ACTIVITY; FALSE POSITIVE RESULT; GENOTOXICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; TOXICITY TESTING;

CELL LINE; CELL PROLIFERATION; COMET ASSAY; DNA DAMAGE; DNA-FORMAMIDOPYRIMIDINE GLYCOSYLASE; ENZYME ACTIVATION; HUMANS; MUTAGENS; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS);

EID: 84876583925     PISSN: 02678357     EISSN: 14643804     Source Type: Journal    
DOI: 10.1093/mutage/get002     Document Type: Article

References (28)

1. Uhl, M., Helma, C. and Knasmüller, S. (1999) Single-cell gel electrophoresis assays with human-derived hepatoma (Hep G2) cells. Mutat. Res., 441, 215-224.
2. Hartmann, A., Plappert, U., Poetter, F. and Suter, W. (2003) Comparative study with the alkaline comet assay and the chromosome aberration test. Mutat. Res., 536, 27-38.
3. Giannotti, E., Vandin, L., Repeto, P. and Comelli, R. (2002) A comparison of the in vitro Comet assay with the in vitro chromosome aberration assay using whole human blood or Chinese hamster lung cells: validation study using a range of novel pharmaceuticals. Mutagenesis, 17, 163-170.
4. Collins, A. R., Duthie, S. J. and Dobson, V. L. (1993) Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis, 14, 1733-1735.
5. Dusinska, M. and Collins, A. R. (1996) Detection of oxidised purines and UV-induced photoproducts in DNA of single cells, by inclusion of lesionspecific enzymes in the comet assay. Alt. Lab. Anim., 24, 405-411.
6. Collins, A. R., Mitchell, D. L., Zunino, A., de Wit, J. and Busch, D. (1997) UV-sensitive rodent mutant cell lines of complementation groups 6 and 8 differ phenotypically from their human counterparts. Environ. Mol. Mutagen., 29, 152-160.
7. Collins, A. R., Dusinská, M. and Horská, A. (2001) Detection of alkylation damage in human lymphocyte DNA with the comet assay. Acta Biochim. Pol., 48, 611-614.
8. Smith, C. C., O'Donovan, M. R. and Martin, E. A. (2006) hOGG1 recognizes oxidative damage using the comet assay with greater specificity than FPG or ENDOIII. Mutagenesis, 21, 185-190.
9. Speit, G., Schütz, P. and Hoffmann, H. (2004) Enhancement of genotoxic effects in the comet assay with human blood samples by aphidicolin. Toxicol. Lett., 153, 303-310.
10. Li, Q., Laval, J. and Ludlum, D. B. (1997) Fpg protein releases a ringopened N-7 guanine adduct from DNA that has been modified by sulfur mustard. Carcinogenesis, 18, 1035-1038.
11. He, Y. H., Xu, Y., Kobune, M., Wu, M., Kelley, M. R. and Martin, W. J. (2002) Escherichia coli FPG and human OGG1 reduce DNA damage and cytotoxicity by BCNU in human lung cells. Am. J. Physiol. Lung Cell Mol. Physiol., 282, L50-L55.
12. Gill, R. D., Cussac, C., Souhami, R. L. and Laval, F. (1996) Increased resistance to N,N',N''-triethylenethiophosphoramide (thiotepa) in cells expressing the Escherichia coli formamidopyrimidine-DNA glycosylase. Cancer Res., 56, 3721-3724.
13. Shaposhnikov, S., Azqueta, A., Henriksson, S., et al. (2010) Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation. Toxicol. Lett., 195, 31-34.
14. Azqueta, A. and Collins, A. R. (2011) The comet assay: a sensitive and quantitative method for analysis of DNA damage. Encyclopedia of Analytical Chemistry. John Wiley and Sons, Chichester. doi:10.1002/9780470027318. a9192
15. Burlinson, B. (2012) The in vitro and in vivo comet assays. In Parry, J. M. and Parry, E. M. (eds), Genetic Toxicology: Principles and Methods, Springer, New York, pp 143-163.
16. Pfuhler, S. and Wolf, H. U. (1996) Detection of DNA-crosslinking agents with the alkaline comet assay. Environ. Mol. Mutagen., 27, 196-201.
17. Galloway, S., Lorge, E., Aardema, M. J. et al. (2011) Workshop summary: top concentration for in vitro mammalian cell genotoxicity assays; and report from working group on toxicity measures and top concentration for in vitro cytogenetics assays (chromosome aberrations and micronucleus). Mutat. Res., 723, 77-83.
18. Kirkland, D. (2010) Evaluation of different cytotoxic and cytostatic measures for the in vitro micronucleus test (MNVit): summary of results in the collaborative trial. Mutat. Res., 702, 139-147.
19. Fowler, P., Whitwell, J., Jeffrey, L., Young, J., Smith, K. and Kirkland, D. (2010) Etoposide; colchicine; mitomycin C and cyclophosphamide tested in the in vitro mammalian cell micronucleus test (MNvit) in Chinese hamster lung (CHL) cells at Covance laboratories; Harrogate UK in support of OECD draft Test Guideline 487. Mutat. Res., 702, 175-180.
20. Fowler, P., Whitwell, J., Jeffrey, L., Young, J., Smith, K. and Kirkland, D. (2010) Cadmium chloride, benzo[a]pyrene and cyclophosphamide tested in the in vitro mammalian cell micronucleus test (MNvit) in the human lymphoblastoid cell line TK6 at Covance laboratories, Harrogate UK in support of OECD draft Test Guideline 487. Mutat. Res., 702, 171-174.
21. EFSA Scientific Committee (2011) Scientific opinion on genotoxicity testing strategies applicable to food and feed safety assessment. EFSA Journal, 9, 2379.
22. OECD (1997) OECD Guideline for the testing of chemicals no. 471. Bacterial reverse mutation test.
23. OECD (2010) OECD Guideline for the testing of chemicals no. 487. In vitro mammalian cell micronucleus test.
24. Kirkland, D. and Speit, G. (2008) Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and noncarcinogens III. Appropriate follow-up testing in vivo. Mutat. Res., 654, 114-132.
25. Collins, A. (1983) DNA repair in ultraviolet-irradiated HeLa cells is disrupted by aphidicolin. The inhibition of repair need not imply the absence of repair synthesis. Biochim. Biophys. Acta, 741, 341-347.
26. Gedik, C. M., Ewen, S. W. and Collins, A. R. (1992) Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int. J. Radiat. Biol., 62, 313-320.
27. Speit, G., Schütz, P., Bonzheim, I., Trenz, K. and Hoffmann, H. (2004) Sensitivity of the FPG protein towards alkylation damage in the comet assay. Toxicol. Lett., 146, 151-158.
28. Kawaguchi, S., Nakamura, T., Yamamoto, A., Honda, G. and Sasaki, Y. F. (2010) Is the comet assay a sensitive procedure for detecting genotoxicity? J. Nucleic Acids, 2010, 541050.