The influence of scoring method on variability in results obtained with the comet assay

Mutagenesis

Volumn 26, Issue 3, 2011, Pages 393-399

  Azqueta, Amaya ^a   Meier, Silja ^a,c   Priestley, Catherine ^b   Gutzkow, Kristine Bjerve ^c   Brunborg, Gunnar ^c   Sallette, Jérôme ^d   Soussaline, Franoise ^d   Collins, Andrew ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b ASTRAZENECA   (United Kingdom)

^c NORWEGIAN INSTITUTE OF PUBLIC HEALTH   (Norway)

^d IMSTAR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; DNA; HYDROGEN PEROXIDE; MESYLIC ACID METHYL ESTER;

ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; INTERMETHOD COMPARISON; LYMPHOBLASTOID CELL; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; SCORING SYSTEM;

CELL LINE; COMET ASSAY; HUMANS; HYDROGEN PEROXIDE; IMAGE PROCESSING, COMPUTER-ASSISTED; LYMPHOCYTES; METHYL METHANESULFONATE; RESEARCH DESIGN;

EID: 79955462525     PISSN: 02678357     EISSN: 14643804     Source Type: Journal    
DOI: 10.1093/mutage/geq105     Document Type: Article

References (7)

1. ESCODD, Gedik, C. M. and Collins, A. Establishing the background level of base oxidation in human lymphocyte DNA: results of an interlaboratory validation study. FASEB J., 19, 82-84.
2. Forchhammer, L., Johanson, C., Loft, S. et al. (2010) Variation in the measurement of DNA damage by comet assay measured by the ECVAG inter-laboratory validation trial. Mutagenesis, 25, 113-123.
3. Shaposhnikov, S., Azqueta, A., Henriksson, S. et al. (2010) Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation. Toxicol. Lett., 195, 31-34.
4. Collins, A., Dusinská, M., Franklin, M. et al. (1997) Comet assay in human biomonitoring studies: reliability, validation, and applications. Environ. Mol. Mutagen., 30, 139-146. (Pubitemid 27440590)
5. Bland, J. M. and Altman, D. G. (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet, 327, 307-310. (Pubitemid 16134762)
6. Collins, A. R., Ma, A. and Duthie, S. J. (1995) The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutat. Res., 336, 69-77.
7. Forchhammer, L., Bräuner, E. V., Folkmann, J. K., Danielsen, P. H., Nielsen, C., Jensen, A., Loft, S., Friis, G. and Møller, P. (2008) Variation in assessment of oxidatively damaged DNA in mononuclear blood cells by the comet assay with visual scoring. Mutagenesis, 23, 223-231.