A simple method for isolating and culturing the rat brain microvascular endothelial cells

Microvascular Research

Volumn 90, Issue , 2013, Pages 199-205

  Liu, Yang ^a,b,c   Xue, Qiang ^a,b,c   Tang, Qing ^a,b,c   Hou, Min ^a,b,c   Qi, Hongyi ^a,b,c   Chen, Gang ^d   Chen, Weihai ^a   Zhang, Jifen ^a,b,c   Chen, Yi ^a,b,c   Xu, Xiaoyu ^a,b,c  

^a SOUTHWEST UNIVERSITY   (China)

^b Chongqing Center for Pharmacodynamics Research and Evaluation   (China)

^c Pharmacology of Chinese Materia Medica   (China)

^d CHONGQING TECHNOLOGY AND BUSINESS UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; CD31 ANTIGEN; CELL ADHESION MOLECULE; GLIAL FIBRILLARY ACIDIC PROTEIN; INTERCELLULAR ADHESION MOLECULE; MULTIDRUG RESISTANCE PROTEIN; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG; VON WILLEBRAND FACTOR;

ANIMAL CELL; ARTICLE; ASTROCYTE; BRAIN CORTEX; CELL GROWTH; CELL ISOLATION; CELL STRUCTURE; CELL ULTRASTRUCTURE; CONTROLLED STUDY; GRAY MATTER; IN VITRO STUDY; MICROVASCULAR ENDOTHELIAL CELL; NERVE CELL CULTURE; NEWBORN; NONHUMAN; PERICYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALIA; RATTUS;

ANIMALS; ANIMALS, NEWBORN; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SEPARATION; CELL SHAPE; CELLS, CULTURED; CEREBRAL CORTEX; ELECTRIC IMPEDANCE; ENDOTHELIAL CELLS; MICROVESSELS; PHENOTYPE; RATS; TIME FACTORS;

EID: 84888429255     PISSN: 00262862     EISSN: 10959319     Source Type: Journal    
DOI: 10.1016/j.mvr.2013.08.004     Document Type: Article

References (31)

1. Abbott N.J., et al. Astrocyte-endothelial interactions at the blood-brain barrier. Nat. Rev. Neurosci. 2006, 7:41-53.
2. Akanuma S.-i, et al. ATP-binding cassette transporter A1 (ABCA1) deficiency does not attenuate the brain-to-blood efflux transport of human amyloid-β peptide (1-40) at the blood-brain barrier. Neurochem. Int. 2008, 52:956-961.
3. Angelis E.D., et al. Endothelial cell biology and culture methods for drug transport studies. Adv. Drug Deliv. Rev. 1996, 18:193-218.
4. Bowman P., et al. Primary culture of capillary endothelium from rat brain. In Vitro Cell. Dev. Biol. Plant 1981, 17:353-362.
5. Brightman M.W., Reese T.S. Junctions between intimately apposed cell membranes in vertebrate brain. J. Cell Biol. 1969, 40:648-677.
6. Calabria A.R., Shusta E.V. A genomic comparison of in vivo and in vitro brain microvascular endothelial cells. J. Cereb. Blood Flow Metab. 2008, 28:135-148.
7. Demeuse P., et al. Compartmentalized coculture of rat brain endothelial cells and astrocytes: a syngenic model to study the blood-brain barrier. J. Neurosci. Methods 2002, 121:21-31.
8. Engelhardt B. Development of the blood-brain barrier. Cell Tissue Res. 2003, 314:119-129.
9. Gaillard P.J., et al. Establishment and functional characterization of an in vitro model of the blood-brain barrier, comprising a co-culture of brain capillary endothelial cells and astrocytes. Eur. J. Pharm. Sci. 2001, 12:215-222.
10. Goldstein G.W., et al. Isolation of metabolically active capillaries from rat-brain. J. Neurochem. 1975, 25:715-717.
11. Guo J.-Y., et al. Cinnamaldehyde reduces IL-1β-induced cyclooxygenase-2 activity in rat cerebral microvascular endothelial cells. Eur. J. Pharmacol. 2006, 537:174-180.
12. Hamm S., et al. Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts. Cell Tissue Res. 2004, 315:157-166.
13. Hayashi K., et al. Effects of hypoxia on endothelial/pericytic co-culture model of the blood-brain barrier. Regul. Pept. 2004, 123:77-83.
14. Hiu T., et al. Tissue plasminogen activator enhances the hypoxia/reoxygenation-induced impairment of the blood-brain barrier in a primary culture of rat brain endothelial cells. Cell. Mol. Neurobiol. 2008, 28:1139-1146.
15. Ichikawa N., et al. Isolation and primary culture of rat cerebral microvascular endothelial cells for studying drug transport in vitro. J. Pharmacol. Toxicol. Methods 1996, 36:45-52.
16. Morofuji Y., et al. Pitavastatin strengthens the barrier integrity in primary cultures of rat brain endothelial cells. Cell. Mol. Neurobiol. 2010, 30:727-735.
17. Nakagawa S., et al. Pericytes from brain microvessels strengthen the barrier integrity in primary cultures of rat brain endothelial cells. Cell. Mol. Neurobiol. 2007, 27:687-694.
18. Nakagawa S., et al. A new blood-brain barrier model using primary rat brain endothelial cells, pericytes and astrocytes. Neurochem. Int. 2009, 54:253-263.
19. Neuwelt E., et al. Strategies to advance translational research into brain barriers. Lancet Neurol. 2008, 7:84-96.
20. Panula P., et al. Evidence for the presence of viable endothelial cells in cultures derived from dissociated rat brain. Cell Mol. Life Sci. 1978, 34:95-97.
21. Pardridge W.M. Drug and gene targeting to the brain with molecular Trojan horses. Nat. Rev. Drug Discov. 2002, 1:131-139.
22. Parkinson F.E., Hacking C. Pericyte abundance affects sucrose permeability in cultures of rat brain microvascular endothelial cells. Brain Res. 2005, 1049:8-14.
23. Persidsky Y., et al. Blood-brain barrier: structural components and function under physiologic and pathologic conditions. J. NeuroImmune Pharm. 2006, 1:223-236.
24. Reese T.S., Karnovsky M.J. Fine structural localization of a blood-brain barrier to exogenous peroxidase. J. Cell Biol. 1967, 34:207-217.
25. Silva R.F., et al. Dissociated primary nerve cell cultures as models for assessment of neurotoxicity. Toxicol. Lett. 2006, 163:1-9.
26. Takata F., et al. Oncostatin M induces functional and structural impairment of blood-brain barriers comprised of rat brain capillary endothelial cells. Neurosci. Lett. 2008, 441:163-166.
27. Weidenfeller C., et al. Murine brain capillary endothelial cells exhibit improved barrier properties under the influence of hydrocortisone. Brain Res. 2005, 1053:162-174.
28. Wolf S. An introduction to the blood-brain barrier. Integr. Physiol. Behav. Sci. 1999, 34:287-288.
29. Wu Z., et al. A simple method for isolation and characterization of mouse brain microvascular endothelial cells. J. Neurosci. Methods 2003, 130:53-63.
30. Zlokovic B.V. The blood-brain barrier in health and chronic neurodegenerative disorders. Neuron 2008, 57:178-201.
31. Zoellner H.F.A., Hunter N. Histochemical identification of the vascular endothelial isoenzyme of alkaline-phosphatase. J. Histochem. Cytochem. 1989, 37:1893-1898.