Isolation and primary culture of rat cerebral microvascular endothelial cells for studying drug transport in vitro

Journal of Pharmacological and Toxicological Methods

Volumn 36, Issue 1, 1996, Pages 45-52

  Ichikawa, Nobuhiro ^a   Naora, Kohji ^a   Hirano, Hidenari ^a   Hashimoto, Michio ^b   Masumura, Sumio ^b   Iwamoto, Kikuo ^a,b  

^a SHIMANE UNIVERSITY   (Japan)

^b SHIMANE UNIVERSITY HOSPITAL   (Japan)

Author keywords

Blood brain barrier; Cerebral; Endothelium; Rat; Tissue culture; Transport

Indexed keywords

2,4 DINITROPHENOL; 3 METHYLGLUCOSE; ALKALINE PHOSPHATASE; BLOOD CLOTTING FACTOR 8; GAMMA GLUTAMYLTRANSFERASE; LOW DENSITY LIPOPROTEIN; MANNITOL; PROLINE;

ANIMAL CELL; ARTICLE; BLOOD BRAIN BARRIER; BRAIN CAPILLARY; CELL CULTURE; CELL ISOLATION; CONTROLLED STUDY; CRYOPRESERVATION; DRUG TRANSPORT; ENDOTHELIUM CELL; ENZYME ACTIVITY; IN VITRO STUDY; MALE; NONHUMAN; RAT; TECHNIQUE;

ANIMALIA;

EID: 0030245605     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/1056-8719(96)00072-X     Document Type: Article

References (32)

1. Abbott NJ, Hughes CCW, Revest PA, Greenwood J (1992) Development and characterisation of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier. J Cell Sci 103:23-37.
2. Amtorp O (1980) Estimation of capillary permeability of inulin, sucrose and mannitol in rat brain cortex. Acta Physiol Scand 110:337-342.
3. Audus KL, Borchardt RT (1986) Characterization of an in vitro blood-brain barrier model system for studying drug transport and metabolism. Pharm Res 3:81-87.
4. Betz AL, Csejtey J, Goldstein GW (1979) Hexose transport and phosphorylation by capillaries isolated from rat brain. Am J Physiol 236:C96-C102.
5. Bowman PD, Betz AL, Ar D, Wolinsky JS, Penney JB, Shivers RR, Goldstein GW (1981) Primary culture of capillary endothelium from rat brain. In Vitro 17:353-362.
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
7. Brightman MW, Reese TS (1969) Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol 40:648-677.
8. Cardelli-Cangiano P, Fiori A, Cangiano C, Barberini F, Allegra P, Peresempio V, Storm R (1987) Isolated brain microvessels as in vitro equivalents of the blood-brain barrier: selective removal by collagenase of the A-system of neutral amino acid transport. J Neurochem 49:1667-1675.
9. Davson H, Zlokovic B, Rakic L, Segal MB (1993) An Introduction to the Blood-brain Barrier. Boca Raton, CRC Press, pp. 1-145.
10. Dehouck M-P, Jolliet-Riant P, Brée F, Fruchart J-C, Cecchelli R, Tillement J-P (1992) Drug transfer across the blood-brain barrier: correlation between in vitro and in vivo models. J Neurochem 58:1790-1797.
11. Djuricic BM, Mrsulja BB (1977) Enzymatic activity of the brain: microvessels vs. total forebrain homogenate. Brain Res 138:561-564.
12. Goldstein GW, Wolinski JS, Csejtey J, Diamond I (1975) Isolation of metabolically active capillaries from rat brain. J Neurochem 25:715-717.
13. Gordon EL, Danielsson PE, Nguyen T-S, Winn HR (1991) A comparison of primary cultures of rat cerebral microvascular endothelial cells to rat aortic endothelial celis. In Vitro Cell Dev Biol 27A:312-326.
14. Hughes CCW, Lantos PL (1989) Uptake of leucine and alanine by cultured cerebral capillary endothelial cells. Brain Res 480:126-132.
15. 14C]proline by isolated rat brain capillaries. J Neurochem 40:317-323.
16. Jaffe EA (1977) Endothelial cells and the biology of Factor VIII. New Engl J Med 296:377-383.
17. Joó F (1992) The cerebral microvessels in culture, an update. J Neurochem 58:1-17.
18. Masereeuw R, Jaehde U, Langemeijer MWE, de Boer AG, Breimer DD (1994) In vitro and in vivo transport of zidovudine (AZT) across the blood-brain barrier and the effect of transport inhibitors. Pharm Res 11:324-330.
19. Orlowski M, Sessa G, Green JP (1974) γ-Glutamyl transpeptidase in brain capillaries: possible site of a blood-brain barrier for amino acids. Science 184:66-68.
20. Panula P, Joó F, Rechardt L (1978) Evidence for the presence of viable endothelial cells in cultures derived from dissociated rat brain. Experientia 15:95-97.
21. Pardridge WM (1988) Recent advances in blood-brain barrier transport. Ann Rev Pharmacol Toxicol 28:25-39.
22. Pardridge WM, Triguero D, Yang J, Cancilla PA (1990) Comparison of in vitro and in vivo models of drug transcytosis through the blood-brain barrier. J Pharmacol Exp Ther 253:884-891.
23. Phillips P, Kumar P, Kumar S, Waghe M (1979) Isolation and characterization of endothelial cells from rat and cow brain white matter. J Anat 129:261-272.
24. Reese TS, Karnovsky MJ (1967) Fine structural localization of a blood-brain barrier to exogenous peroxidase. J Cell Biol 34:207-217.
25. Spatz M, Bembry J, Dodson RF, Hervonen H, Murray MR (1980) Endothelial cell cultures derived from isolated cerebral microvessels. Brain Res 191:577-582.
26. Spector R (1990) Drug transport in the central nervous system: role of carriers. Pharmacology 40:1-7.
27. Takakura Y, Kuentzel SL, Raub TJ, Davies A, Baldwin SA, Borchardt RT (1991) Hexose uptake in primary cultures of bovine brain microvessel endothelial cells. I. Basic characteristics and effects of D-glucose and insulin. Biochim Biophys Acta 1070:1-10.
28. Terasaki T, Takakuwa S, Moritani S, Tsuji A (1991) Transport of monocarboxylic acids at the blood-brain barrier: studies with monolayers of primary cultured bovine brain capillary endothelial cells. J Pharmacol Exp Ther 258:932-937.
29. Voyta JC, Via DP, Butterfield CE, Zetter BR (1984) Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J Cell Biol 99:2034-2040.
30. Walker I, Nicholls D, Irwin WJ, Freeman S (1994) Drug delivery via active transport at the blood-brain barrier: II. Investigation of monocarboxylic acid transport in vitro. Int J Pharm 108:225-232.
31. Williams SK, Gillis JF, Matthews MA, Wagner RC, Bitensky MW (1980) Isolation and characterization of brain endothelial cells: morphology and enzyme activity. J Neurochem 35:374-381.
32. Zoellner HFA, Hunter N (1989) Histochemical identification of the vascular endothelial isoenzyme of alkaline phosphatase. J Histochem Cytochem 37:1893-1898.