Sortase-tag expressed protein ligation: Combining protein purification and site-specific bioconjugation into a single step

Analytical Chemistry

Volumn 85, Issue 22, 2013, Pages 11090-11097

  Warden Rothman, Robert ^a   Caturegli, Ilaria ^a   Popik, Vladimir ^b   Tsourkas, Andrew ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EXPRESSED PROTEIN LIGATION; FLUORESCENT LABELS; NON-STOICHIOMETRIC; OPTIMAL REACTION CONDITION; PROOF OF PRINCIPLES; PROTEIN PURIFICATION; PROTEIN SCAFFOLDS; TARGETED THERAPEUTICS;

LIGANDS; MOLECULAR IMAGING; NANOPARTICLES; PROTEINS; PURIFICATION; REMOVAL;

SCAFFOLDS (BIOLOGY);

AMINOACYLTRANSFERASE; AZIDE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; ENHANCED GREEN FLUORESCENT PROTEIN; EPIDERMAL GROWTH FACTOR RECEPTOR 2; GREEN FLUORESCENT PROTEIN; NANOPARTICLE; PEPTIDE FRAGMENT; SORTASE A;

ANIMAL; ARTICLE; CHEMISTRY; CLICK CHEMISTRY; FLUORESCENCE; METABOLISM; METHODOLOGY; MOUSE; NUCLEAR MAGNETIC RESONANCE IMAGING; WESTERN BLOTTING;

AMINOACYLTRANSFERASES; ANIMALS; AZIDES; BACTERIAL PROTEINS; BLOTTING, WESTERN; CLICK CHEMISTRY; CYSTEINE ENDOPEPTIDASES; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; MAGNETIC RESONANCE IMAGING; MICE; NANOPARTICLES; PEPTIDE FRAGMENTS; RECEPTOR, ERBB-2;

EID: 84888320793     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac402871k     Document Type: Article

References (34)

1. Rana, S.; Yeh, Y.-C.; Rotello, V. M. Curr. Opin. Chem. Biol. 2010, 14, 828-834
2. Sapsford, K. E.; Algar, W. R.; Berti, L.; Gemmill, K. B.; Casey, B. J.; Oh, E.; Stewart, M. H.; Medintz, I. L. Chem. Rev. 2013, 113, 1904-2074
3. Stephanopoulos, N.; Francis, M. B. Nat. Chem. Biol. 2011, 7, 876-884
4. Thorek, D. L.; Elias, D. R.; Tsourkas, A. Mol. Imaging 2009, 8, 221-229
5. Stephanopoulos, N.; Francis, M. B. Nat. Chem. Biol. 2011, 7, 876-884
6. Fernandez-Suarez, M.; Baruah, H.; Martinez-Hernandez, L.; Xie, K. T.; Baskin, J. M.; Bertozzi, C. R.; Ting, A. Y. Nat. Biotechnol. 2007, 25, 1483-1487
7. Slavoff, S. A.; Chen, I.; Choi, Y. A.; Ting, A. Y. J. Am. Chem. Soc. 2008, 130, 1160-1162
8. Keppler, A.; Gendreizig, S.; Gronemeyer, T.; Pick, H.; Vogel, H.; Johnsson, K. Nat. Biotechnol. 2003, 21, 86-89
9. Los, G. V.; Encell, L. P.; McDougall, M. G.; Hartzell, D. D.; Karassina, N.; Zimprich, C.; Wood, M. G.; Learish, R.; Ohana, R. F.; Urh, M.; Simpson, D.; Mendez, J.; Zimmerman, K.; Otto, P.; Vidugiris, G.; Zhu, J.; Darzins, A.; Klaubert, D. H.; Bulleit, R. F.; Wood, K. V. ACS Chem. Biol. 2008, 3, 373-382
10. Gautier, A.; Juillerat, A.; Heinis, C.; Correa, I. R., Jr.; Kindermann, M.; Beaufils, F.; Johnsson, K. Chem. Biol. 2008, 15, 128-136
11. Meier, J. L.; Mercer, A. C.; Rivera, H., Jr.; Burkart, M. D. J. Am. Chem. Soc. 2006, 128, 12174-12184
12. Chong, S.; Mersha, F. B.; Comb, D. G.; Scott, M. E.; Landry, D.; Vence, L. M.; Perler, F. B.; Benner, J.; Kucera, R. B.; Hirvonen, C. a.; Pelletier, J. J.; Paulus, H.; Xu, M. Q. Gene 1997, 192, 271-281
13. Dawson, P. E.; Kent, S. B. Annu. Rev. Biochem. 2000, 69, 923-960
14. Elias, D. R.; Cheng, Z.; Tsourkas, A. Small 2010, 6, 2460-2468
15. Erathodiyil, N.; Ying, J. Y. Acc. Chem. Res. 2011, 44, 925-935
16. Leung, M. K. M.; Hagemeyer, C. E.; Johnston, A. P. R.; Gonzales, C.; Kamphuis, M. M. J.; Ardipradja, K.; Such, G. K.; Peter, K.; Caruso, F. Angew. Chem., Int. Ed. 2012, 51, 7132-7136
17. Proft, T. Biotechnol. Lett. 2010, 32, 1-10
18. Spirig, T.; Weiner, E. M.; Clubb, R. T. Mol. Microbiol. 2011, 82, 1044-1059
19. Race, P. R.; Bentley, M. L.; Melvin, J. A.; Crow, A.; Hughes, R. K.; Smith, W. D.; Sessions, R. B.; Kehoe, M. A.; McCafferty, D. G.; Banfield, M. J. J. Biol. Chem. 2009, 284, 6924-6933
20. Mao, H. Protein Express. Purif. 2004, 37, 253-263
21. Kobashigawa, Y.; Kumeta, H.; Ogura, K.; Inagaki, F. J. Biomol. NMR 2009, 43, 145-150
22. Orlova, A.; Magnusson, M.; Eriksson, T. L.; Nilsson, M.; Larsson, B.; Hoiden-Guthenberg, I.; Widstrom, C.; Carlsson, J.; Tolmachev, V.; Stahl, S.; Nilsson, F. Y. Cancer Res. 2006, 66, 4339-4348
23. Qi, S.; Miao, Z.; Liu, H.; Xu, Y.; Feng, Y.; Cheng, Z. Bioconjugate Chem. 2012, 23, 1149-1156
24. Zong, Y.; Bice, T. W.; Ton-That, H.; Schneewind, O.; Narayana, S. V. J. Biol. Chem. 2004, 279, 31383-31389
25. McRae, S. R.; Brown, C. L.; Bushell, G. R. Protein Express. Purif. 2005, 41, 121-127
26. Offterdinger, M.; Georget, V.; Girod, A.; Bastiaens, P. I. J. Biol. Chem. 2004, 279, 36972-36981
27. Elias, D. R.; Poloukhtine, A.; Popik, V.; Tsourkas, A. Nanomed.-Nanotechnol. 2013, 9, 194-201
28. Junutula, J. R.; Raab, H.; Clark, S.; Bhakta, S.; Leipold, D. D.; Weir, S.; Chen, Y.; Simpson, M.; Tsai, S. P.; Dennis, M. S.; Lu, Y.; Meng, Y. G.; Ng, C.; Yang, J.; Lee, C. C.; Duenas, E.; Gorrell, J.; Katta, V.; Kim, A.; McDorman, K.; Flagella, K.; Venook, R.; Ross, S.; Spencer, S. D.; Lee Wong, W.; Lowman, H. B.; Vandlen, R.; Sliwkowski, M. X.; Scheller, R. H.; Polakis, P.; Mallet, W. Nat. Biotechnol. 2008, 26, 925-932
29. Bellucci, J. J.; Amiram, M.; Bhattacharyya, J.; McCafferty, D.; Chilkoti, A. Angew. Chem., Int. Ed. 2013, 1-7
30. Steinhagen, M.; Zunker, K.; Nordsieck, K.; Beck-Sickinger, A. G. Bioorg. Med. Chem. 2013, 21, 3504-3510
31. Fong, B. a.; Wu, W.-Y.; Wood, D. W. Trends Biotechnol. 2010, 28, 272-279
32. Norris, V.; Grant, S.; Freestone, P.; Canvin, J.; Sheikh, F. N.; Toth, I.; Trinei, M.; Modha, K.; Norman, R. I. J. Bacteriol. 1996, 178, 3677-3682
33. Alberty, R.; Hammes, G. J. Phys. Chem. 1958, 62, 154-159
34. Zhou, G. Q.; Zhong, W. Z. Eur. J. Biochem. 1982, 128, 383-387