Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Fusarium oxysporum f. sp. niveum in soil

FEMS Microbiology Letters

Volumn 349, Issue 2, 2013, Pages 127-134

  Peng, Jun ^a   Zhan, Yuanfeng ^a   Zeng, Fanyun ^a   Long, Haibo ^a   Pei, Yuelin ^a   Guo, Jianrong ^a  

^a INSTITUTE OF TROPICAL BIOSCIENCE AND BIOTECHNOLOGY   (China)

Author keywords

Detection of fungi; Fusarium wilt of watermelon; Real time PCR; RealAmp assay

Indexed keywords

GENOMIC DNA;

FUNGAL DETECTION; FUNGUS ISOLATION; FUNGUS SPORE; FUSARIUM OXYSPORUM; GENE AMPLIFICATION; INTERMETHOD COMPARISON; LETTER; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME FLUORESCENCE LOOP MEDIATED ISOTHERMAL AMPLIFICATION ASSAY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOIL INOCULATION;

CITRULLUS LANATUS VAR. LANATUS; FUNGI; FUSARIUM; FUSARIUM OXYSPORUM; FUSARIUM OXYSPORUM F. SP. NIVEUM;

DETECTION OF FUNGI; FUSARIUM WILT OF WATERMELON; REAL-TIME PCR; REALAMP ASSAY;

DNA, FUNGAL; FUSARIUM; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SOIL MICROBIOLOGY;

EID: 84888061607     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/1574-6968.12305     Document Type: Letter

References (25)

1. Bekele B, Hodgetts J, Tomlinson J, Boonham N, Nikolic P, Swarbrick P & Dickinson M (2011) Use of a real-time LAMP isothermal assay for detecting 16SrII and 16SrXII phytoplasmas in fruit and weeds of the Ethiopian Rift Valley. Plant Pathol 60: 345-355.
2. Besri M (2008) Cucurbits grafting as alternative to methyl bromide for cucurbits production in Morocco. Proceedings of the 14th International Research Conference on Methyl Bromide Alternatives and Emissions Reduction, Orlando, pp. 60-61.
3. Brierley JL, Stewart JA & Lees AK (2009) Quantifying potato pathogen DNA in soil. Appl Soil Ecol 41: 234-238.
4. Cullen DW, Lees AK, Toth IK & Duncan JM (2001) Conventional PCR and real-time quantitative PCR detection of Helminthos porium solaniin soil and on potato tubers. Eur J Plant Pathol 107: 387-398.
5. Goto M, Honda E, Ogura A, Nomoto A & Hanaki K (2009) Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxyl naphthol blue. Biotechniques 46: 167-172.
6. Heap JW & McKay AC (2004) Spatial distribution of soilborne disease inoculum DNA in cereal crops and implications for soil sampling protocols. Proceedings of the Third Australasian Soilborne Diseases Symposium (Ophel-Keller K & Hall B, eds) p. 106. Department of Primary Industry and Resources South Australia, Adelaide.
7. Kaneko H, Kawana T, Fukushima E & Suzutani T (2007) Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J Biochem Biophys Methods 70: 499-501.
8. Lees AK, Sullivan L, Lynott JS & Cullen DW (2012) Development of a quantitative real-time PCR assay for Phytophthora infestans and its applicability to leaf, tuber and soil samples. Plant Pathol 61: 867-876.
9. Li S & Hartman GL (2003) Molecular detection of Fusarium solani f. sp. glycines in soybean roots and soil. Plant Pathol 52: 74-83.
10. Lin YH, Chen KS, Chang JY, Wan YL, Hsu CC, Huang JW & Chang PFL (2010) Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan. New Biotechnol 27: 409-418.
11. Lin Z, Zhang Y, Zhang H, Zhou Y, Cao J & Zhou J (2012) Comprison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Vet Parasitol 185: 296-300.
12. Lucchi NW, Demas A, Narayanan J, Sumari D, Kabanywanyi A, Kachur SP, Barnwell JW & Udhayakumar V (2010) Real-time fluorescence loop-mediated isothermal amplification for the diagnosis of malaria. PLoS ONE 5: e13733.
13. Martyn RD & McLanghlin RJ (1983) Effects of inoculum concentration on the apparent resistance of watermelons to Fusarium oxysporum f.sp. niveum. Plant Dis 67: 493-495.
14. Nagamine K, Watanabe K, Ohtsuka K, Hase T & Notomi T (2001) Loop-mediated isothermal amplification reaction using a nondenatured template. Clin Chem 47: 1742-1743.
15. Nash SM & Snyder WC (1962) Quantitative estimates by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology 52: 567-572.
16. Njiru ZK, Yeboah-Manu D, Stinear TP & Fyfed JAM (2012) Rapid and sensitive detection of Mycobacterium ulcerans by use of a loop-mediated isothermal amplification test. J Clin Microbiol 50: 1737.
17. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N & Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28: e63.
18. Ophel-Keller K, McKay A, Hartley D, Herdina & Curran J (2008) Development of routine DNA-based testing service for soil-borne diseases in Australia. Austral Plant Pathol 37: 243-253.
19. Peng J, Zhang J, Xia Z, Li Y, Huang J & Fan Z (2012) Rapid and sensitive detection of banana bunchy top virus by loop-mediated isothermal amplification. J Virol Methods 185: 254-258.
20. Tomita N, Mori Y, Kanda H & Notomi T (2008) Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3: 877-882.
21. Tomlinson JA, Dickinson MJ & Boonham N (2010) Detection of Botrytis cinerea by loop-mediated isothermal amplification. Lett Appl Microbiol 51: 650-657.
22. Wallenhammar AC, Almquist C, Söderström M & Jonsson A (2012) In-field distribution of Plasmodiophora brassicae measured using quantitative real-time PCR. Plant Pathol 61: 16-28.
23. Yang B, Wang X, Li H, Li G, Cao Z & Cheng X (2011) Comprison of loop-mediated isothermal amplification and real-time PCR for the diagnosis of tuberculous pleurisy. Lett Appl Microbiol 53: 525-531.
24. Zhang Z, Zhang J, Wang Y & Zheng X (2005) Molecular detection of Fusarium oxysporum f. sp. niveum and Mycosphaerella melonis in infected plant tissues and soil. FEMS Microbiol Lett 249: 39-47.
25. Zoheir KM & Allam AA (2011) A rapid improved method for sexing embryo of water buffalo. Theriogenology 76: 83-87.