Detection of Botrytis cinerea by loop-mediated isothermal amplification

Letters in Applied Microbiology

Volumn 51, Issue 6, 2010, Pages 650-657

  Tomlinson, J A ^a   Dickinson, M J ^b   Boonham, N ^a  

^a FOOD AND ENVIRONMENT RESEARCH AGENCY   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Botrytis; Grey mould; Isothermal amplification; Quantitative detection; Rapid methods

Indexed keywords

ISOTHERMS; TESTBEDS;

BOTRYTIS; BOTRYTIS CINEREA; GREY MOLD; ISOTHERMAL AMPLIFICATIONS; LOOP MEDIATED ISOTHERMAL AMPLIFICATIONS; LOOP-MEDIATED ISOTHERMAL AMPLIFICATIONS; QUANTITATIVE DETECTION; RAPID METHOD; SENSITIVE METHOD; SENSITIVITY AND SPECIFICITY;

DNA;

FUNGAL DNA; RIBOSOME DNA;

BIOASSAY; DETECTION METHOD; FLOWER; FUNGAL DISEASE; FUNGUS; GENETIC ANALYSIS; ISOTHERM; LABORATORY METHOD; REAL TIME; STORAGE;

ARTICLE; BACTERIUM DETECTION; BOTRYTIS CINEREA; CROSS REACTION; DNA EXTRACTION; FUNGUS CULTURE; GENE AMPLIFICATION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; PETAL; REAL TIME POLYMERASE CHAIN REACTION; ROSE; SENSITIVITY AND SPECIFICITY;

BOTRYOTINIA FUCKELIANA; BOTRYOTINIA PELARGONII; BOTRYTIS;

EID: 78349253085     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2010.02949.x     Document Type: Article

References (30)

1. Bossi, R. and Dewey, F.M. (1992) Development of a monoclonal antibody-based immunodetection assay for Botrytis cinerea. Plant Pathol 41, 472-482.
2. Brouwer, M., Lievens, B., Van Hemelrijck, W., Van den Ackerveken, G., Cammue, B.P.A. and Thomma, B.P.H.J. (2003) Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR. FEMS Microbiol Lett 228, 241-248.
3. Cadle-Davidson, L. (2008) Monitoring pathogenesis of natural Botrytis cinerea infections in developing grape berries. Am J Enol Vitic 59, 387-395.
4. Celik, M., Kalpulov, T., Zutahy, Y., Ish-shalom, S., Lurie, S. and Lichter, A. (2009) Quantitative and qualitative analysis of Botrytis inoculated on table grapes by qPCR and antibodies. Postharvest Biol Technol 52, 235-239.
5. Danks, C. and Boonham, N. (2007) Purification method and kits. Patent WO/2007/104962.
6. Dewey, F.M. and Meyer, U. (2004) Rapid, quantitative tube immunoassays for on-site detection of Botrytis, Aspergillus and Penicillium antigens in grape juice. Anal Chim Acta 513, 11-19.
7. Fukuta, S., Kato, S., Yoshida, K., Mizukami, Y., Ishida, A., Ueda, J., Kanbe, M. and Ishimoto, Y. (2003) Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction. J Virol Methods 112, 35-40.
8. Gachon, C. and Saindrenan, P. (2004) Real-time PCR monitoring of fungal development in Arabidopsis thaliana infected by Alternaria brassicola and Botrytis cinerea. Plant Physiol Biochem 42, 367-371.
9. Goto, M., Honda, E., Ogura, A., Notomo, A. and Hanaki, K.I. (2009) Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. BioTechniques 46, 167-172.
10. Hartman, L.J., Coyne, S.R. and Norwood, D.A. (2005) Development of a novel internal positive control for TaqMan based assays. Mol Cell Probes 19, 51-59.
11. Iwamoto, T., Sonobe, T. and Hayashi, K. (2003) Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples. J Clin Microbiol 41, 2616-2622.
12. Kaneko, H., Kawana, T., Fukushima, E. and Suzutani, T. (2007) Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J Biochem Biophys Methods 70, 499-501.
13. Kiatpathomchai, W., Jaroenram, W., Arunrut, N., Jitrapakdee, S. and Flegel, T.W. (2008) Shrimp Taura syndrome virus detection by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick. J Virol Methods 153, 214-217.
14. Lane, C.R., Hobden, E., Walker, L., Barton, V.C., Inman, A.J., Hughes, K.J.D., Swan, H., Colyer, A. et al. (2007) Evaluation of a rapid diagnostic field test kit for identification of Phytophthora species including P. ramorum and P. kernoviae at the point of inspection. Plant Pathol 56, 828-835.
15. Maeda, H., Kokeguchi, S., Fujimoto, C., Tanimoto, I., Yoshizumi, W., Nishimura, F. and Takashiba, S. (2005) Detection of periodontal pathogen Porphyromonas gingivalis by loop-mediated isothermal amplification method. FEMS Immunol Med Microbiol 43, 233-239.
16. Mehli, L., Kjellsen, T.D., Dewey, F.M. and Hietala, A.M. (2005) A case study from the interaction of strawberry and Botrytis cinerea highlights the benefits of comonitoring both partners at genomic and mRNA level. New Phytol 168, 465-474.
17. Meyer, U.M., Spotts, R.A. and Dewey, F.M. (2000) Detection and quantification of Botrytis cinerea by ELISA in pear stems during cold storage. Plant Dis 84, 1099-1103.
18. Mori, Y., Nagamine, K., Tomita, N. and Notomi, T. (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289, 150-154.
19. Mori, Y., Kitao, M., Tomita, N. and Notomi, T. (2004) Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods 59, 145-157.
20. Nagamine, K., Watanabe, K., Ohtsuka, K., Hase, T. and Notomi, T. (2001) Loop-mediated isothermal amplification reaction using a nondenatured template. Clin Chem 47, 1742-1743.
21. Nagamine, K., Hase, T. and Notomi, T. (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16, 223-229.
22. Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. and Hase, T. (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28, e63.
23. Rigotti, S., Gindro, K., Richter, H. and Viret, O. (2002) Characterizarion of molecular markers for specific and sensitive detection of Botrytis cinerea Pers.: Fr. in strawberry (Fragaria×ananassa Duch.) using PCR. FEMS Microbiol Lett 209, 169-174.
24. Spotts, R.A., Wallis, K.M., Serdani, M., O'Gorman, D.T. and Sholberg, P.L. (2008) Methodology for determining relationships between inoculum concentration of Botrytis cinerea and Penicillium expansum and stem end decay of pear fruit. Plant Dis 92, 451-455.
25. Spotts, R.A., Serdani, M., Wallis, K.M., Walter, M., Harris-Virgin, T., Spotts, K., Sugar, D., Xiao, C.L. et al. (2009) At-harvest prediction of grey mould risk in pear fruit in long-term cold storage. Crop Prot 28, 414-420.
26. Staats, M., Van Baarlen, P. and Van Kan, J.A.L. (2005) Molecular phylogeny of the plant pathogenic genus Botrytis and evolution of host specificity. Mol Biol Evol 22, 333-346.
27. Suarez, M.B., Walsh, K., Boonham, N., O'Neill, T., Pearson, S. and Barker, I. (2005) Development of real-time PCR (TaqMan) assays for the detection and quantification of Botrytis cinerea in planta. Plant Physiol Biochem 43, 890-899.
28. Tani, H., Teramura, T., Adachi, K., Tsuneda, S., Kurata, S., Nakamura, K., Kanagawa, T. and Noda, N. (2007) Technique for quantitative detection of specific DNA sequences using alternately binding quenching probe competitive assay combined with loop-mediated isothermal amplification. Anal Chem 79, 5608-5613.
29. Tomlinson, J.A., Dickinson, M.J. and Boonham, N. (2010) Rapid detection of Phytophthora ramorum and P. kernoviae by two-minute DNA extraction followed by isothermal amplification and amplicon detection by generic lateral flow device. Phytopathology 100, 143-149.
30. Williamson, B., Tudzynsk, B., Tudzynski, P. and Van Kan, J.A.L. (2007) Botrytis cinerea: the cause of grey mould disease. Mol Plant Pathol 8, 561-580.