Prolonged expansion of human nucleus pulposus cells expressing human telomerase reverse transcriptase mediated by lentiviral vector

Journal of Orthopaedic Research

Volumn 32, Issue 1, 2014, Pages 159-166

  Wu, Jianhong ^a   Wang, Deli ^a   Ruan, Dike ^a   He, Qing ^a   Zhang, Yan ^a   Wang, Chaofeng ^a   Xin, Hongkui ^a   Xu, Cheng ^a   Liu, Yue ^a  

^a NAVY GENERAL HOSPITAL   (China)

Author keywords

degenerative disc diseases; gene therapy; human nucleus pulposus cells; human telomerase reverse transcriptase; lentiviral vector; nucleus pulposus cells; telomerase

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; TELOMERASE; TELOMERASE REVERSE TRANSCRIPTASE;

ARTICLE; CELL AGING; CELL EXPANSION; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ACTIVITY; EXTRACELLULAR MATRIX; GENE EXPRESSION; HUMAN; HUMAN CELL; NUCLEUS PULPOSUS; PRIORITY JOURNAL; TRANSGENE;

DEGENERATIVE DISC DISEASES; GENE THERAPY; HUMAN NUCLEUS PULPOSUS CELLS; HUMAN TELOMERASE REVERSE TRANSCRIPTASE; LENTIVIRAL VECTOR; NUCLEUS PULPOSUS CELLS; TELOMERASE;

AGGRECANS; CELL PROLIFERATION; CELLS, CULTURED; COLLAGEN TYPE II; ENZYME ACTIVATION; GENETIC THERAPY; GENETIC VECTORS; HUMANS; INTERVERTEBRAL DISC; INTERVERTEBRAL DISC DEGENERATION; KARYOTYPE; LENTIVIRUS; TELOMERASE; TELOMERE; TRANSDUCTION, GENETIC;

EID: 84888055764     PISSN: 07360266     EISSN: 1554527X     Source Type: Journal    
DOI: 10.1002/jor.22474     Document Type: Article

References (29)

1. Singh K, Masuda K, Thonar EJ., 2009. Age-related changes in the extracellular matrix of nucleus pulposus and anulus fibrosus of human intervertebral disc. Spine 34: 10-16.
2. Preradovic A, Kleinpeter G, Feichtinger H., 2005. Quantitation of collagen I, collagen II and aggrecan mRNA and expression of the corresponding proteins in human nucleus pulposus cells in monolayer cultures. Cell Tissue Res 321: 459-464.
3. Roberts S, Evans H, Trivedi J., 2006. Histology and pathology of the human intervertebral disc. J Bone Joint Surg Am 88: 10-14.
4. Gruber HE, Hanley EN Jr., 2000. Human disc cells in monolayer vs 3D culture:cell shape, division and matrix formation. BMC Musculoskelet Disord 1: 1.
5. Zhdanova NS, Minina IuM, Rubtsov NB., 2012. Mammalian telomere biology. Mol Biol (Mosk) 46: 539-555.
6. Gomez DE, Armando RG, Farina HG., 2012. Telomere structure and telomerase in health and disease (review). Int J Oncol 41: 1561-1569.
7. Chung SA, Wei AQ, Connor DE., 2007. Nucleus pulposus cellular longevity by telomerase gene therapy. Spine 32: 1188-1196.
8. Wu J, Wang D, Zhang C., 2011. Extending the activities of human nucleus pulposus cells with recombinant adeno-associated virus vector-mediated human telomerase reverse transcriptase gene transfer. Tissue Eng Part A 17: 2407-2415.
9. Perez-Martinez FC, Guerra J, Posadas I., 2011. Barriers to non-viral vector-mediated gene delivery in the nervous system. Pharm Res 28: 1843-1858.
10. Grieger JC, Samulski RJ., 2005. Adeno-associated virus as a gene therapy vector:vector development, production and clinical applications. Adv Biochem Eng Biotechnol 99: 119-145.
11. Goncalves MA., 2005. Adeno-associated virus: from defective virus to effective vector. Virol J 2: 43.
12. Durand S, Cimarelli A., 2011. The inside out of lentiviral vectors. Viruses 3: 132-159.
13. Murakami Y, Ikeda Y, Yonemitsu Y., 2010. Inhibition of choroidal neovascularization via brief subretinal exposure to a newly developed lentiviral vector pseudotyped with Sendai viral envelope proteins. Hum Gene Ther 21: 199-209.
14. Copreni E, Nicolis E, Tamanini A., 2009. Late generation lentiviral vectors: evaluation of inflammatory potential in human airway epithelial cells. Virus Res 144: 8-17.
15. Rossi JJ., 2009. Dotting the I's and crossing the T's: integration analyses in transduced patient T cells. Mol Ther 17: 756-757.
16. Sinn PL, Sauter SL, McCray PB Jr., 2005. Gene therapy progress and prospects: development of improved lentiviral and retroviral vectors-design, biosafety, and production. Gene Ther 12: 1089-1098.
17. Throm RE, Ouma AA, Zhou S., 2009. Efficient construction of producer cell lines for a SIN lentiviral vector for SCID-X1 gene therapy by concatemeric array transfection. Blood 113: 5104-5110.
18. Song H, Yang PC., 2010. Construction of shRNA lentiviral vector. N Am J Med Sci 2: 598-601.
19. Mayshar Y, Ben-David U, Lavon N., 2010. Identification and classification of chromosomal aberrations in human induced pluripotent stem cells. Cell Stem Cell 7: 521-531.
20. Taghizadeh RR, Sherley JL., 2008. CFP and YFP, but not GFP, provide stable fluorescent marking of rat hepatic adult stem cells. J Biomed Biotechnol 2008: 453590.
21. Liu HS, Jan MS, Chou CK., 1999. Is green fluorescent protein toxic to the living cells ? Biochem Biophys Res Commun 260: 712-717.
22. Herbst F, Ball CR, Tuorto F., 2012. Extensive methylation of promoter sequences silences lentiviral transgene expression during stem cell differentiation in vivo. Mol Ther 20: 1014-1021.
23. Cong Y, Shay JW., 2008. Actions of human telomerase beyond telomeres. Cell Res 18: 725-732.
24. Melin BS, Nordfjall K, Andersson U., 2012. hTERT cancer risk genotypes are associated with telomere length. Genet Epidemiol 36: 368-372.
25. Danet-Desnoyers GA, Luongo JL, Bonnet DA., 2005. Telomerase vaccination has no detectable effect on SCID-repopulating and colony-forming activities in the bone marrow of cancer patients. Exp Hematol 33: 1275-1280.
26. Nourbakhsh M, Golestani A, Zahrai M., 2010. Androgens stimulate telomerase expression, activity and phosphorylation in ovarian adenocarcinoma cells. Mol Cell Endocrinol 330: 10-16.
27. Zhao CF, Hu HY, Meng L., 2010. Immortalization of bovine mammary epithelial cells alone by human telomerase reverse transcriptase. Cell Biol Int 34: 579-586.
28. Tsuruga Y, Kiyono T, Matsushita M., 2008. Establishment of immortalized human hepatocytes by introduction of HPV16 E6/E7 and hTERT as cell sources for liver cell-based therapy. Cell Transplant 17: 1083-1094.
29. Raty JK, Lesch HP, Wirth T., 2008. Improving safety of gene therapy. Curr Drug Saf 3: 46-53.