Human disc cells in monolayer vs 3D culture: Cell shape, division and matrix formation

BMC Musculoskeletal Disorders

Volumn 1, Issue , 2000, Pages 1-6

  Gruber, Helen E ^a   Hanley Jr , Edward N ^a  

^a CAROLINAS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHONDROITIN SULFATE; COLLAGEN TYPE 1; COLLAGEN TYPE 2; KERATAN SULFATE; MATRIGEL; PLATELET DERIVED GROWTH FACTOR;

ARTICLE; CELL CULTURE; CELL DIVISION; CELL FUNCTION; CELL PROLIFERATION; CELL SHAPE; CELL TYPE; CONTROLLED STUDY; EXTRACELLULAR MATRIX; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; INTERVERTEBRAL DISK; MITOSIS;

EID: 0001779594     PISSN: 14712474     EISSN: 14712474     Source Type: Journal    
DOI: 10.1186/1471-2474-1-1     Document Type: Article

References (24)

1. Chelberg MK, Banks GM, Geiger DF, Oegema TR Jr: Identification of heterogeneous cell populations in normal human intervertebral disc. J. Anat. 1955, 186:43-53
2. Poiraudeau S, Monteiro I, Anract P, Blanchard O, Revel M, Corvol MT: Phenotypic characteristics of rabbit intervertebral disc cells - Comparison with cartilage cells from the same animals. Spine 1999, 24:837-844
3. Chiba K, Andersson GBJ, Masuda K, Thonar EJMA: Metabolism of the extracellular matrix formed by intervertebral disc cells cultured in alginate. Spine 1997, 22:2885-2893
4. Chiba K, Andersson GBJ, Masuda K, Momohara S, Williams JM, Thonar EJMA: A new culture system to study the metabolism of the intervertebral disc in vitro. Spine 1998, 23:1821-1827
5. Osada R, Ohshima H, Ishihara H, Yudoh K, Sakai K, Matsui H, Tsuji H: Autocrine/paracrine mechanism of insulin-like growth factor-1 secretion, and the effect of insulin-like growth factor-1 on proteoglycan synthesis in bovine intervertebral discs. J. Orthop. Res. 1996, 14:690-699
6. Ichimura K, Tsuji H, Matsui H, Makiyama N: Cell culture of the intervertebral disc of rats: Factors influencing culture, proteoglycan, collagen, and deoxyribonucleic acid synthesis. J. Spinal Disorders 1991, 4:428-436
7. Yoo JU, Papy RS, Malemud CJ: Suppression of proteoglycan synthesis in chondrocyte cultures derived from canine intervertebral disc. Spine 1992, 17:221-224
8. Maldonado BA, Oegema TR Jr: Initial characterization of the metabolism of intervertebral disc cells encapsulated in microspheres. J. Orthopaedic Res. 1992, 10:677-690
9. Thompson JP, Oegema TR Jr, Bradford DS: Stimulation of mature canine intervertebral disc by growth factors. Spine 1991, 16:253-260
10. Gruber HE, Hanley EN Jr: Anti-apoptotic effects of IGF-1 and PDGF on human intervertebral disc cells in vitro. Spine 2000, 25:2153-2157
11. Gruber HE, Fisher EC Jr, Desai B, Stasky AA, Hoelscher G, Hanley EN: Human intervertebral disc cells from the annulus: Three-dimensional culture in agarose or alginate and responsiveness to TGF-β1. Exp. Cell Res. 1997, 235:13-21
12. Desai BJ, Gruber HE, Hanley EN Jr: The influence of Matrigel™ or growth factor reduced Matrigel™ on human intervertebral disc cell growth and proliferation. Histol. Histopathol. 1999, 14:359-368
13. Gruber HE, Stasky AA, Hanley EN Jr: Characterization and phenotypic stability of human disc cells in vitro. Matrix Biol. 1997, 16:285-288
14. Thompson JP, Pearce RH, Schechter MT, Adams ME, Tsang IKY, Bishop PB: Preliminary evaluation of a scheme for grading the gross morphology of the human intervertebral disc. Spine 1990, 15:411-415
15. Schwob JE, Szumowski KEM, Stasky AA: Olfactory sensory neurons are trophically dependent on the olfactory bulb for their prolonged survival. J Neurosci. 1992, 12:3896-3919
16. Ramp WK, Dillaman RM, Lenz LG, Gay DM, Roer RD, Ballard TA: A serum substitute promotes osteoblast-like phenotypic expression in cultured cells from chick calvariae. Bone and Mineral 1991, 15:1-17
17. Benya PD, Shaffer JD: Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in aga-rose gels. Cell 1982, 30:215-224
18. Benya PD, Padilla SR, Nimni ME: Independent regulation of collagen types by chondrocytes during the loss of differentiated function in culture. Cell 1978, 15:1313-1321
19. Von der Mark K, Gauss V, von der Mark H, Müller P: Relationship between cell shape and type of collagen synthesized as chondrocytes lose their cartilage phenotype in culture. Nature 1977, 267:531-532
20. Glowacki J, Trepman E, Folkman J: Cell shape and phenotypic expression in chondrocytes. Proc. Soc. Exp. Biol. Med. 1983, 172:93-98
21. Desai BJ, Gruber HE: Anti-apoptotic actions of cytokines in mammalian cells. Proc. Soc. Exp. Biol. Med. 1999, 221:1-13
22. Deuel TF: Polypeptide growth factors: Roles in normal and abnormal cell growth. Ann. Rev. Cell Biol. 1996, 3:443-492
23. Barres BA, Hart IK, Coles HSR, Burne VF, Voyvodic JR, Richardson WS, Raff MC: Cell death and control of cell survival in the oli-godendrocyte lineage. Cell 1992, 70:31-46
24. Deuel TF, Huang JS, Huang SS, Stroobant P, Waterfield MD: Expression of a platelet-derived growth factor-like protein in simian sarcoma virus transformed cells. Science 1983, 221:1348-1350