Geraniol hydroxylase and hydroxygeraniol oxidase activities of the CYP76 family of cytochrome P450 enzymes and potential for engineering the early steps of the (seco)iridoid pathway

Metabolic Engineering

Volumn 20, Issue , 2013, Pages 221-232

  Höfer, René ^a   Dong, Lemeng ^b   André, François ^c   Ginglinger, Jean François ^a   Lugan, Raphael ^a   Gavira, Carole ^a   Grec, Sebastien ^d   Lang, Gerhard ^e   Memelink, Johan ^f   Van Der Krol, Sander ^b   Bouwmeester, Harro ^b   Werck Reichhart, Danièle ^a  

^a INST DE BIOL MOLEC DES PLANTES   (France)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c SERVICE DE CHIMIE BIOORGANIQUE ET DE MARQUAGE   (France)

^d UNIV LILLE   (France)

^e Philip Morris Products SA   (Switzerland)

^f LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Catharanthus roseus; Cytochrome P450; Geraniol oxidation; In planta enzyme assay; Multifunctional enzyme; Pathway reconstitution

Indexed keywords

BIOCHEMISTRY; ENZYMES; HISTOLOGY; HYDROXYLATION; TISSUE;

ARABIDOPSIS THALIANA; CATHARANTHUS ROSEUS; CYTOCHROME P450; CYTOCHROME P450 ENZYMES; ENZYME ASSAYS; PATHWAY RECONSTITUTION; PATHWAY RECONSTRUCTION; THERAPEUTIC APPLICATION;

MONOTERPENES;

CYTOCHROME P450; GERANIOL; GERANIOL HYDROXYLASE; HYDROXYGERANIOL OXIDASE; IRIDOID; OXIDOREDUCTASE; OXYGENASE; UNCLASSIFIED DRUG;

ARABIDOPSIS THALIANA; ARTICLE; CATHARANTHUS ROSEUS; CONTROLLED STUDY; DRUG HYDROXYLATION; ENZYME ACTIVITY; ENZYME ENGINEERING; LEAF TISSUE; NONHUMAN; PLANT TISSUE; PRIORITY JOURNAL;

ARABIDOPSIS THALIANA; CATHARANTHUS ROSEUS;

CATHARANTHUS ROSEUS; CYTOCHROME P450; GERANIOL OXIDATION; IN PLANTA ENZYME ASSAY; MULTIFUNCTIONAL ENZYME; PATHWAY RECONSTITUTION;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; CYTOCHROME P-450 ENZYME SYSTEM; IRIDOID GLUCOSIDES; OXIDATION-REDUCTION;

EID: 84888045086     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2013.08.001     Document Type: Article

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