Calnexin silencing in mouse neonatal cardiomyocytes induces Ca2+ cycling defects, ER stress, and apoptosis

Journal of Cellular Physiology

Volumn 229, Issue 3, 2014, Pages 374-383

  Bousette, Nicolas ^a,b,c   Abbasi, Cynthia ^a,b   Chis, Roxana ^a,b   Gramolini, Anthony O ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c MONTREAL HEART INSTITUTE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATING TRANSCRIPTION FACTOR 6; APOPTOSIS SIGNAL REGULATING KINASE 1; CAFFEINE; CALCIUM; CALCIUM CHANNEL; CALNEXIN; CASPASE 3; CASPASE 9; ENDOPLASMIC RETICULUM OXIDASE 1 ALPHA; GLUCOSE REGULATED PROTEIN 78; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 153; LENTIVIRUS VECTOR; MESSENGER RNA; OXIDOREDUCTASE; SARCOPLASMIC RETICULUM CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; X BOX BINDING PROTEIN 1;

ANIMAL CELL; APOPTOSIS; ARTICLE; BIOGEOCHEMICAL CYCLING; CALCIUM TRANSPORT; CONTROLLED STUDY; ENDOPLASMIC RETICULUM STRESS; ENZYME ACTIVATION; GENE EXPRESSION; GENE SILENCING; HEART MUSCLE CELL; MORPHOLOGY; MOUSE; NONHUMAN; PRIORITY JOURNAL; SARCOMERE; STIMULATION; VIRAL GENE DELIVERY SYSTEM;

ACTIVATING TRANSCRIPTION FACTOR 6; ANIMALS; ANIMALS, NEWBORN; APOPTOSIS; CAFFEINE; CALCIUM CHANNELS, L-TYPE; CALCIUM SIGNALING; CALNEXIN; CASPASE 3; CASPASE 9; CELL SURVIVAL; DNA-BINDING PROTEINS; ENDOPLASMIC RETICULUM; ENDOPLASMIC RETICULUM STRESS; GENETIC VECTORS; GLYCOPROTEINS; HEAT-SHOCK PROTEINS; HEK293 CELLS; HUMANS; LENTIVIRUS; MAP KINASE KINASE KINASE 5; MICE; MYOCYTES, CARDIAC; RNA INTERFERENCE; RNA, MESSENGER; SARCOPLASMIC RETICULUM CALCIUM-TRANSPORTING ATPASES; TIME FACTORS; TRANSCRIPTION FACTOR CHOP; TRANSCRIPTION FACTORS; TRANSDUCTION, GENETIC; TRANSFECTION;

EID: 84888031482     PISSN: 00219541     EISSN: 10974652     Source Type: Journal    
DOI: 10.1002/jcp.24459     Document Type: Article

References (26)

1. Bousette N, Chugh S, Fong V, Isserlin R, Kim KH, Volchuk A, Backx PH, Liu P, Kislinger T, MacLennan DH, Emili A, Gramolini AO. 2010. Constitutively active calcineurin induces cardiac endoplasmic reticulum stress and protects against apoptosis that is mediated by alpha-crystallin-B. Proc Natl Acad Sci USA 107:18481-18486.
2. Brini M, Manni S, Pierobon N, Du GG, Sharma P, MacLennan DH, Carafoli E. 2005. Ca2+ signaling in HEK-293 and skeletal muscle cells expressing recombinant ryanodine receptors harboring malignant hyperthermia and central core disease mutations. J Biol Chem 280:15380-15389.
3. Brockmeier A, Williams DB. 2006. Potent lectin-independent chaperone function of calnexin under conditions prevalent within the lumen of the endoplasmic reticulum. Biochemistry 45:12906-12916.
4. Chen X, Zhang X, Kubo H, Harris DM, Mills GD, Moyer J, Berretta R, Potts ST, Marsh JD, Houser SR. 2005. Ca2+ influx-induced sarcoplasmic reticulum Ca2+ overload causes mitochondrial-dependent apoptosis in ventricular myocytes. Circ Res 97:1009-1017.
5. Coe H, Bedard K, Groenendyk J, Jung J, Michalak M. 2008. Endoplasmic reticulum stress in the absence of calnexin. Cell Stress Chaperones 13:497-507.
6. Delom F, Emadali A, Cocolakis E, Lebrun JJ, Nantel A, Chevet E. 2007. Calnexin-dependent regulation of tunicamycin-induced apoptosis in breast carcinoma MCF-7 cells. Cell Death Differ 14:586-596.
7. Denzel A, Molinari M, Trigueros C, Martin JE, Velmurgan S, Brown S, Stamp G, Owen MJ. 2002. Early postnatal death and motor disorders in mice congenitally deficient in calnexin expression. Mol Cell Biol 22:7398-7404.
8. Fu HY, Minamino T, Tsukamoto O, Sawada T, Asai M, Kato H, Asano Y, Fujita M, Takashima S, Hori M, Kitakaze M. 2008. Overexpression of endoplasmic reticulum-resident chaperone attenuates cardiomyocyte death induced by proteasome inhibition. Cardiovasc Res 79:600-610.
9. Guo R, Ma H, Gao F, Zhong L, Ren J. 2009. Metallothionein alleviates oxidative stress-induced endoplasmic reticulum stress and myocardial dysfunction. J Mol Cell Cardiol 47:228-237.
10. Gupta GS. 2012. Lectins in quality control: Calnexin and calretculin. In: Gupta GS, editor. Animal lectins: Form, function and clinical applications. Wien: Springer-Verlag. pp 29-56.
11. Kang PM, Haunstetter A, Aoki H, Usheva A, Izumo S. 2000. Morphological and molecular characterization of adult cardiomyocyte apoptosis during hypoxia and reoxygenation. Circ Res 87:118-125.
12. Kraus A, Groenendyk J, Bedard K, Baldwin TA, Krause KH, Dubois-Dauphin M, Dyck J, Rosenbaum EE, Korngut L, Colley NJ, Gosgnach S, Zochodne D, Todd K, Agellon LB, Michalak M. 2010. Calnexin deficiency leads to dysmyelination. J Biol Chem 285:18928-18938.
13. Lancel S, Joulin O, Favory R, Goossens JF, Kluza J, Chopin C, Formstecher P, Marchetti P, Neviere R. 2005. Ventricular myocyte caspases are directly responsible for endotoxin-induced cardiac dysfunction. Circulation 111:2596-2604.
14. Ma Y, Hendershot LM. 2004. The role of the unfolded protein response in tumour development: Friend or foe? Nat Rev Cancer 4:966-977.
15. Martelli AM, Zweyer M, Ochs RL, Tazzari PL, Tabellini G, Narducci P, Bortul R. 2001. Nuclear apoptotic changes: An overview. J Cell Biochem 82:634-646.
16. McCullough KD, Martindale JL, Klotz LO, Aw TY, Holbrook NJ. 2001. Gadd153 sensitizes cells to endoplasmic reticulum stress by down-regulating Bcl2 and perturbing the cellular redox state. Mol Cell Biol 21:1249-1259.
17. Mesaeli N, Nakamura K, Zvaritch E, Dickie P, Dziak E, Krause KH, Opas M, MacLennan DH, Michalak M. 1999. Calreticulin is essential for cardiac development. J Cell Biol 144:857-868.
18. Nickson P, Toth A, Erhardt P. 2007. PUMA is critical for neonatal cardiomyocyte apoptosis induced by endoplasmic reticulum stress. Cardiovasc Res 73:48-56.
19. Nishitoh H, Matsuzawa A, Tobiume K, Saegusa K, Takeda K, Inoue K, Hori S, Kakizuka A, Ichijo H. 2002. ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats. Genes Dev 16:1345-1355.
20. Papp S, Dziak E, Kabir G, Backx P, Clement S, Opas M. 2010. Evidence for calreticulin attenuation of cardiac hypertrophy induced by pressure overload and soluble agonists. Am J Pathol 176:1113-1121.
21. Reeve JL, Szegezdi E, Logue SE, Ni Chonghaile T, O'Brien T, Ritter T, Samali A. 2007. Distinct mechanisms of cardiomyocyte apoptosis induced by doxorubicin and hypoxia converge on mitochondria and are inhibited by Bcl-xL. J Cell Mol Med 11:509-520.
22. Ryan T, Sharma P, Ignatchenko A, MacLennan DH, Kislinger T, Gramolini AO. 2011. Identification of novel ryanodine receptor 1 (RyR1) protein interaction with calcium homeostasis endoplasmic reticulum protein (CHERP). J Biol Chem 286:17060-17068.
23. Truettner JS, Hu K, Liu CL, Dietrich WD, Hu B. 2009. Subcellular stress response and induction of molecular chaperones and folding proteins after transient global ischemia in rats. Brain Res 1249:9-18.
24. Wang XZ, Lawson B, Brewer JW, Zinszner H, Sanjay A, Mi LJ, Boorstein R, Kreibich G, Hendershot LM, Ron D. 1996. Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153). Mol Cell Biol 16:4273-4280.
25. Zinszner H, Kuroda M, Wang X, Batchvarova N, Lightfoot RT, Remotti H, Stevens JL, Ron D. 1998. CHOP is implicated in programmed cell death in response to impaired function of the endoplasmic reticulum. Genes Dev 12:982-995.
26. Zuppini A, Groenendyk J, Cormack LA, Shore G, Opas M, Bleackley RC, Michalak M. 2002. Calnexin deficiency and endoplasmic reticulum stress-induced apoptosis. Biochemistry 41:2850-2858.