Antibody mediated CDCP1 degradation as mode of action for cancer targeted therapy

Molecular Oncology

Volumn 7, Issue 6, 2013, Pages 1142-1151

  Kollmorgen, Gwendlyn ^a   Niederfellner, Gerhard ^a   Lifke, Alexander ^a   Spohn, GloriaJ ^a   Rieder, Natascha ^a   Vega Harring, Suzana ^a   Bauss, Frieder ^a   Burtscher, Helmut ^a   Lammers, Reiner ^b   Bossenmaier, Birgit ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Antibody therapy; CDCP1; Contact inhibition; Src

Indexed keywords

ANTIBODY; CUB DOMAIN CONTAINING PROTEIN 1; MEMBRANE PROTEIN; PROTEASOME; PROTEIN; TRITON X 100; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CANCER INHIBITION; CELL MEMBRANE; CELL STRAIN 3T3; CONTROLLED STUDY; DIMERIZATION; DOWN REGULATION; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LIPID RAFT; MOLECULARLY TARGETED THERAPY; MOUSE; NONHUMAN; ONCOGENE SRC; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; THERAPY; TUMOR GROWTH; TUMOR VOLUME; WESTERN BLOTTING; XENOGRAFT;

ANTIBODY THERAPY; CDCP1; CONTACT INHIBITION; SRC;

ANIMALS; ANTIBODIES, NEOPLASM; ANTIGENS, CD; ANTIGENS, NEOPLASM; CELL ADHESION MOLECULES; CELL LINE, TUMOR; CELL MEMBRANE; HUMANS; MEMBRANE GLYCOPROTEINS; MICE; NEOPLASM PROTEINS; NEOPLASMS, EXPERIMENTAL; NIH 3T3 CELLS; PROTEOLYSIS; XENOGRAFT MODEL ANTITUMOR ASSAYS;

EID: 84887994979     PISSN: 15747891     EISSN: 18780261     Source Type: Journal    
DOI: 10.1016/j.molonc.2013.08.009     Document Type: Article

References (21)

1. Alvares S.M., Dunn C.A., Brown T.A., Wayner E.E., Carter W.G. The role of membrane microdomains in transmembrane signaling through the epithelial glycoprotein Gp140/CDCP1. Biochim. Biophys. Acta 2008, 1780.
2. Awakura Y., Nakamura E., Takahashi T., Kotani H., Mikami Y., Kadowaki T., Myoumoto A., Akiyama H., Ito N., Kamoto T., Manabe T., Nobumasa H., Tsujimoto G., Ogawa O. Microarray-based identification of CUB-domain containing protein 1 as a potential prognostic marker in conventional renal cell carcinoma. J.Cancer Res. Clin. Oncol. 2008, 134:1363-1369.
3. Benes C.H., Wu N., Elia A.E., Dharia T., Cantley L.C., Soltoff S.P. The C2 domain of PKCdelta is a phosphotyrosine binding domain. Cell 2005, 121:271-280.
4. Brown T.A., Yang T.M., Zaitsevskaia T., Xia Y., Dunn C.A., Sigle R.O., Knudsen B., Carter W.G. Adhesion or plasmin regulates tyrosine phosphorylation of a novel membrane glycoprotein p80/gp140/CUB domain-containing protein 1 in epithelia. J.Biol. Chem. 2004, 279:14772-14783.
5. Buhring H.J., Kuci S., Conze T., G.Rathke, Bartolovic K., Grunebach F., Scherl-Mostageer M., Brummendorf T.H., Schweifer N., Lammers R. CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and nonhematopoietic origin. Stem Cells 2004, 22:334-343.
6. Cao H., Chen J., Krueger E.W., McNiven M.A. SRC-mediated phosphorylation of dynamin and cortactin regulates the "constitutive" endocytosis of transferrin. Mol. Cell Biol. 2010, 30:781-792.
7. Cooper J.A., Qian H. A mechanism for SRC kinase-dependent signaling by noncatalytic receptors. Biochemistry 2008, 47:5681-5688.
8. Deryugina E.I., Conn E.M., Wortmann A., Partridge J.J., Kupriyanova T.A., Ardi V.C., Hooper J.D., Quigley J.P. Functional role of cell surface CUB domain-containing protein 1 in tumor cell dissemination. Mol. Cancer Res. 2009, 7:1197-1211.
9. He Y., Wortmann A., Burke L.J., Reid J.C., Adams M.N., bdul-Jabbar I., Quigley J.P., Leduc R., Kirchhofer D., Hooper J.D. Proteolysis-induced N-terminal ectodomain shedding of the integral membrane glycoprotein CUB domain-containing protein 1 (CDCP1) is accompanied by tyrosine phosphorylation of its C-terminal domain and recruitment of Src and PKCdelta. J.Biol. Chem. 2010, 285:26162-26173.
10. Hooper J.D., Zijlstra A., Aimes R.T., Liang H., Claassen G.F., Tarin D., Testa J.E., Quigley J.P. Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135kDa cell surface phosphorylated glycoprotein antigen. Oncogene 2003, 22:1783-1794.
11. Ikeda J., Oda T., Inoue M., Uekita T., Sakai R., Okumura M., Aozasa K., Morii E. Expression of CUB domain containing protein (CDCP1) is correlated with prognosis and survival of patients with adenocarcinoma of lung. Cancer Sci. 2009, 100:429-433.
12. Ikeda J.I., Morii E., Kimura H., Tomita Y., Takakuwa T., Hasegawa J.I., Kim Y.K., Miyoshi Y., Noguchi S., Nishida T., Aozasa K. Epigenetic regulation of the expression of the novel stem cell marker CDCP1 in cancer cells. J.Pathol. 2006, 210:75-84.
13. Kapp K., Siemens J., Weyrich P., Schulz J.B., Haring H.U., Lammers R. Extracellular domain splice variants of a transforming protein tyrosine phosphatase alpha mutant differentially activate Src-kinase dependent focus formation. Genes Cells 2007, 12:63-73.
14. Kollmorgen G., Bossenmaier B., Niederfellner G., Haring H.U., Lammers R. Structural requirements for cub domain containing protein 1 (CDCP1) and Src dependent cell transformation. PLoS. One 2012, 7:e53050.
15. Miyazawa Y., Uekita T., Hiraoka N., Fujii S., Kosuge T., Kanai Y., Nojima Y., Sakai R. CUB domain-containing protein 1, a prognostic factor for human pancreatic cancers, promotes cell migration and extracellular matrix degradation. Cancer Res. 2010, 70:5136-5146.
16. Scherl-Mostageer M., Sommergruber W., Abseher R., Hauptmann R., Ambros P., Schweifer N. Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer. Oncogene 2001, 20:4402-4408.
17. Siva A.C., Wild M.A., Kirkland R.E., Nolan M.J., Lin B., Maruyama T., Yantiri-Wernimont F., Frederickson S., Bowdish K.S., Xin H. Targeting CUB domain-containing protein 1 with a monoclonal antibody inhibits metastasis in a prostate cancer model. Cancer Res. 2008, 68:3759-3766.
18. Spassov D.S., Baehner F.L., Wong C.H., McDonough S., Moasser M.M. The transmembrane src substrate Trask is an epithelial protein that signals during anchorage deprivation. Am. J. Pathol. 2009, 174:1756-1765.
19. Uekita T., Jia L., Narisawa-Saito M., Yokota J., Kiyono T., Sakai R. CUB domain-containing protein 1 is a novel regulator of anoikis resistance in lung adenocarcinoma. Mol. Cell Biol. 2007, 27:7649-7660.
20. Uekita T., Tanaka M., Takigahira M., Miyazawa Y., Nakanishi Y., Kanai Y., Yanagihara K., Sakai R. CUB-domain-containing protein 1 regulates peritoneal dissemination of gastric scirrhous carcinoma. Am. J. Pathol. 2008, 172:1729-1739.
21. Wilde A., Beattie E.C., Lem L., Riethof D.A., Liu S.H., Mobley W.C., Soriano P., Brodsky F.M. EGF receptor signaling stimulates SRC kinase phosphorylation of clathrin, influencing clathrin redistribution and EGF uptake. Cell 1999, 96:677-687.