Testosterone induced apoptosis in colon cancer cells is regulated by PI3K/Rac1 signaling

Asian Journal of Andrology

Volumn 15, Issue 6, 2013, Pages 831-834

  Alkahtani, Saad ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

actin; apoptosis; colon cancer cell; testosterone

Indexed keywords

ACTIN; ANDROGEN RECEPTOR; GUANOSINE TRIPHOSPHATASE; MEMBRANE RECEPTOR; PHOSPHATIDYLINOSITOL 3 KINASE; RAC1 PROTEIN; TESTOSTERONE; WORTMANNIN;

APOPTOSIS; ARTICLE; CELL STRAIN CACO 2; COLON CANCER; CONTROLLED STUDY; ENZYME ACTIVATION; HUMAN; HUMAN CELL; MALE; RECEPTOR UPREGULATION; REGULATORY MECHANISM; SIGNAL TRANSDUCTION;

APOPTOSIS; CACO-2 CELLS; HUMANS; PHOSPHATIDYLINOSITOL 3-KINASES; RAC1 GTP-BINDING PROTEIN; SIGNAL TRANSDUCTION; TESTOSTERONE;

EID: 84887231051     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1038/aja.2013.68     Document Type: Article

References (30)

1. Falkenstein F, Norman AW, Wehling W, Clin J. Mannheim classification of nongenomically initiated (rapid) steroid action(s). Endocrinol Metab 2000; 85: 2072-5. (Pubitemid 32269322)
2. Hatzoglou A, Kampa M, Kogia C, Charalampopoulos I, Theodoropoulos PA et al. Membrane androgen receptor activation induces apoptotic regression of human prostate cancer cells invitro and in vivo.J Clin Endocrinol Metab 2005; 90: 893-903. (Pubitemid 40279214)
3. Heinlein CA, Chang C. Androgen receptor in prostate cancer. Endocr Rev 2004; 25: 276-308. (Pubitemid 38501396)
4. Papakonstanti EA, Kampa M, Castanas E, Stournaras C. A rapid, nongenomic, signaling pathway regulates the actin reorganization induced by activation of membrane testosterone receptors. Mol Endocrinol 2003; 17: 870-81. (Pubitemid 36539577)
5. Kallergi G, Agelaki S, Markomanolaki H, Georgoulias V, Stournaras C. Activation of FAK/PI3K/Rac1 signaling controls actin reorganization and inhibits cell motility in human cancer cells. Cell Physiol Biochem 2007; 20: 977-86. (Pubitemid 350059740)
6. Kampa M, Kogia C, Theodoropoulos PA, Anezinis P, Charalampopoulos I et al. Activation of membrane androgen receptors potentiates the antiproliferative effects of paclitaxel on human prostate cancer cells. Mol Cancer Ther 2006; 5: 1342-51. (Pubitemid 43881328)
7. Papadopoulou N, Charalampopoulos I, Alevizopoulos K, Gravanis A, Stournaras C. Rho/ROCK/actin signaling regulates membrane androgen receptor induced apoptosis in prostate cancer cells. Exp Cell Res 2008; 314: 3162-74.
8. Papadopoulou N, Papakonstanti EA, Kallergi G, Alevizopoulos K, Stournaras C. Membrane androgen receptor activation in prostate and breast tumor cells: molecular signaling and clinical impact. IUBMB Life 2009; 61: 56-61.
9. Papadopoulou N, Charalampopoulos I, Anagnostopoulou V, Konstantinidis G, Föller M et al. Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU-145 prostate cancer cells. Mol Cancer 2008; 7: 88.
10. Lieberherr M, Grosse B. Androgens increase intracellular calcium concentration and inositol 1, 4, 5-trisphosphate and diacylglycerol formation via a pertussis toxin-sensitive G-protein. J Biol Chem 1994; 269: 7217-23.
11. Sun YH, Gao X, Tang YJ, Xu CL, Wang LH. Androgens induce increases in intracellular calcium via a G protein-coupled receptor in LNCaP prostate cancer cells. J Androl 2006; 27: 671-8. (Pubitemid 44520808)
12. Gu S, Papadopoulou EM, Gehring O, Nasir K, Dimas SK et al. Functional membrane androgen receptors in colon tumors triggers pro-apoptotic responses in vitro and reduce drastically tumor incidence in vivo. Mol Cancer 2009; 8: 114.
13. Gu S, Papadopoulou N, Nasir K, Föller M, Alevizopoulos K et al. Activation of membrane androgen receptors in colon cancer inhibits the prosurvival signals Akt/Bad in vitro and in vivo and blocks migration via vinculin/actin signaling. Mol Med 2011; 17: 48-58.
14. Schmidt EM, Gu S, Anagnostopoulou V, Alevizopoulos K, Föller M et al. Serum-and glucocorticoid-dependent kinase-1-induced cell migration is dependent on vinculin and regulated by membrane androgen receptor. FEBS J 2012; 279: 1231-42.
15. Gu S, Kounenidakis M, Schmidt EM, Deshpande D, Alkahtani S et al. Rapid activation of FAK/mTOR/p70S6K/PAK1-signaling controls the early testosterone-induced actin reorganization in colon cancer cells. Cell Signal 2013; 25: 66-73.
16. Kampa M, Papakonstanti EA, Hatzoglou A, Stathopoulos EN, Stournaras C et al. The human prostate cancer cell line LNCaP bears functional membrane testosterone receptors that increase PSA secretion and modify actin cytoskeleton. FASEB J 2002; 16: 1429-31.
17. Stournaras C, Stiakaki E, Koukouritaki S, Theodoropoulos PA, Kalmanti M et al. Altered actin polymerization dynamics in various malignant cell types: evidence for differential sensitivity to Cytochalasin B. Biochem Pharmacol1996; 52: 1339-46. (Pubitemid 26366357)
18. Papakonstanti EA, Stournaras C. Actin cytoskeleton architecture and signalling in osmosensing. Methods Enzymol 2007; 428: 227-40.
19. Papakonstanti E, Castanas E, Emmanouel DS, Hartig R, Stournaras C. Early alterations of actin cytoskeleton in OK cells by opioids. J Cell Biochem 1998; 70: 60-9. (Pubitemid 28310920)
20. Vanhaesebroeck B, Alessi DR. The PI3K-PDK1 connection: more than just a road to PKB. Biochem J 2000; 346: 561-76. (Pubitemid 30171014)
21. Michels G, Hoppe UC. Rapid actions of androgens. Front Neuroendocrinol 2008; 29: 182-98.
22. Kampa M, Pelekanou V, Castanas E. Membrane-initiated steroid action in breast and prostate cancer. Steroids 2008; 73: 953-60.
23. Benten WP, Lieberherr M, Giese G, Wrehlke C, Stamm O et al. Functional testosterone receptors in plasma membranes of T cells. FASEB J 1999; 13: 123-33. (Pubitemid 29038282)
24. Benten WP, Lieberherr M, Stamm O, Wrehlke C, Guo Z et al. Testosterone signaling through internalizable surface receptors in androgen receptor-free macrophages. Mol Biol Cell 1999; 10: 3113-23. (Pubitemid 29489549)
25. Wang Z, Liu L, Hou J, Wen D, Yan C et al. Rapid membrane effect of testosterone in LNCaP cells. Urol Int 2008; 81: 353-9.
26. Gatson JW, Kaur P, Singh M. Dihydrotestosterone differentially modulates the mitogen-activated protein kinase and the phosphoinositide 3-kinase/Akt pathways through the nuclear and novel membrane androgen receptor in C6 cells. Endocrinology 2006; 147: 2028-34.
27. Kampa M, Nifli AP, Charalampopoulos I, Alexaki VI, Theodoropoulos PA et al. Opposing effects of estradiol-and testosterone-membrane binding sites on T47D breast cancer cell apoptosis. Exp Cell Res 2005; 307: 41-51. (Pubitemid 40732732)
28. Alexaki VI, Charalampopoulos I, Kampa M, Nifli AP, Hatzoglou A et al. Activation of membrane estrogen receptors induce pro-survival kinases. J Steroid Biochem Mol Biol 2006; 98: 97-110. (Pubitemid 43153782)
29. Somjen D, Kohen F, Gayer B, Kulik T, Knoll E et al. Role of putative membrane receptors in the effect of androgens on human vascular cell growth. J Endocrinol 2004; 180: 97-106. (Pubitemid 38180761)
30. Foradori CD, Weiser MJ, Handa RJ. Non-genomic actions of androgens Front Neuroendocrinol2008; 29: 169-81.