Culturing of human nasal epithelial cells at the air liquid interface

Journal of Visualized Experiments

Volumn , Issue 80, 2013, Pages

  Müller, Loretta ^a   Brighton, Luisa E ^a   Carson, Johnny L ^a   Fischer II , William A ^a,b   Jaspers, Ilona ^a,b  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

Air pollution; Cell culture models; Cellular biology; Ciliated; Co culture models; Epithelium; Issue 80; Nasal epithelium

Indexed keywords

EID: 84886865193     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50646     Document Type: Article

References (30)

1. Swamy, M., Jamora, C., Havran, W., & Hayday, A. Epithelial decision makers: in search of the 'epimmunome'. Nat. Immunol. 11, 656-665, doi:10.1038/ni.1905 (2010).
2. Vareille, M., Kieninger, E., Edwards, M.R., & Regamey, N. The airway epithelium: soldier in the fight against respiratory viruses. Clin. Microbio.l Rev. 24, 210-229, doi:10.1128/CMR.00014-10 (2011).
3. Muller, L. & Jaspers, I. Epithelial cells, the switchboard of respiratory immune defense responses: Effects of air pollutants. Swiss Med. Wkly. (2012).
4. Sanders, C.J., Doherty, P.C., & Thomas, P.G. Respiratory epithelial cells in innate immunity to influenza virus infection. Cell Tissue Res. 343, 13-21, doi:10.1007/s00441-010-1043-z (2011).
5. Robertson, M.J. Role of chemokines in the biology of natural killer cells. J. Leukoc. Biol. 71, 173-183 (2002).
6. Adachi, M., Matsukura, S., Tokunaga, H., & Kokubu, F. Expression of cytokines on human bronchial epithelial cells induced by influenza virus A. Int. Arch. Allergy Immunol. 113, 307-311 (1997).
7. Borchers, M.T., Harris, N.L., Wesselkamper, S.C., Vitucci, M., & Cosman, D. NKG2D ligands are expressed on stressed human airway epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 291, L222-231, doi:10.1152/ajplung.00327.2005 (2006).
8. Groh, V., et al. Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells. Nat. Immunol. 2, 255-260, doi:10.1038/85321 (2001).
9. Obeidy, P. & Sharland, A.F. NKG2D and its ligands. Int J. Biochem. Cell Biol. 41, 2364-2367, doi:10.1016/j.biocel.2009.07.005 (2009).
10. Horvath, K.M., Brighton, L.E., Zhang, W., Carson, J.L., & Jaspers, I. Epithelial Cells From Smokers Modify Dendritic Cell Responses in the Context of Influenza Infection 1. Am. J. Resp. Cell Mol. (2010).
11. Muller, L.B. LE; Jaspers, I. Ozone exposed epithelial cells modify co-cultured natural killer cells. AJP Lung. (in press).
12. Turi, J.L., et al. Oxidative stress activates anion exchange protein 2 and AP-1 in airway epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 283, L791-798, doi:10.1152/ajplung.00398.2001 (2002).
13. Zimmermann, G.S., Neurohr, C., Villena-Hermoza, H., Hatz, R., & Behr, J. Anti-inflammatory effects of antibacterials on human Bronchial epithelial cells. Respir. Res. 10, 89, doi:10.1186/1465-9921-10-89 (2009).
14. Lopez-Souza, N., Avila, P.C., & Widdicombe, J.H. Polarized cultures of human airway epithelium from nasal scrapings and bronchial brushings. In Vitro Cell Dev. Biol. Anim. 39, 266-269, doi:10.1290/1543-706X(2003)039<0266:PCOHAE>2.0.CO;2 (2003).
15. Gray, T.E., Guzman, K., Davis, C.W., Abdullah, L.H., & Nettesheim, P. Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am. J. Respir. Cell Mo.l Biol. 14, 104-112 (1996).
16. Adler, K.B., Schwarz, J.E., Whitcutt, M.J., & Wu, R. A New Chamber System for Maintaining Differentiated Guinea-Pig Respiratory Epithelial-Cells between Air and Liquid-Phases. Biotechniques. 5, 462-&(1987).
17. Whitcutt, M.J., Adler, K.B. & Wu, R. A biphasic chamber system for maintaining polarity of differentiation of cultured respiratory tract epithelial cells. In Vitro Cell. Dev. Biol. 24, 420-428 (1988).
18. Jaspers, I., et al. Reduced expression of IRF7 in nasal epithelial cells from smokers after infection with influenza. Am. J. Respir. Cell Mol. Biol. 43, 368-375, doi:10.1165/rcmb.2009-0254OC (2010).
19. Muller, L., Brighton, L.E., & Jaspers, I. Ozone exposed epithelial cells modify cocultured natural killer cells. Am. J. Physiol. Lung Cell Mol. Physiol. 304, L332-341, doi:10.1152/ajplung.00256.2012 (2013).
20. Ochs, M. & Weibel, E.R. In Fishman's Pulmoary Diseases and Disorders. Volume 4, A.P. Fishman et al., eds. 23-69 Mc Graw Hill, New York, NY (2008).
21. Kesic, M.J., Meyer, M., Bauer, R. I J. Exposure to Ozone Modulates Human Airway Protease/Antiprotease Balance Contributing to Increased Influenza A Infection. PLoS ONE. 7, e35108, doi:10.1371/journal.pone.0035108 (2012).
22. Jaspers, I., et al. Diesel exhaust enhances influenza virus infections in respiratory epithelial cells. Toxicol. Sci. 85, 990-1002 (2005).
23. Jaspers, I., Flescher, E., & Chen, L.C. Ozone-induced IL-8 expression and transcription factor binding in respiratory epithelial cells. Am. J. Physiol. 272, L504-511 (1997).
24. Blume, C., et al. Barrier responses of human bronchial epithelial cells to grass pollen exposure. Eur. Respir. J. doi:10.1183/09031936.00075612 (2012).
25. Bauer, R.N., et al. Influenza enhances caspase-1 in bronchial epithelial cells from asthmatic volunteers and is associated with pathogenesis. J. Allergy Clin. Immunol. 130, 958-967 e914, doi:10.1016/j.jaci.2012.07.013 (2012).
26. Ostrowski, L.E., Stewart, D., & Hazucha, M. Interferon gamma stimulates accumulation of gas phase nitric oxide in differentiated cultures of normal and cystic fibrosis airway epithelial cells. Lung. 190, 563-571, doi:10.1007/s00408-012-9395-7 (2012).
27. Jardim, M.J., Fry, R.C., Jaspers, I., Dailey, L. & Diaz-Sanchez, D. Disruption of microRNA expression in human airway cells by diesel exhaust particles is linked to tumorigenesis-associated pathways. Environ. Health Perspect. 117, 1745-1751, doi:10.1289/ehp.0900756 (2009).
28. Horvath, K.M., Brighton, L.E., Herbst, M., Noah, T.L., & Jaspers, I. Live Attenuated Influenza Virus (LAIV) induces different mucosal T cell function in nonsmokers and smokers. Clin. Immunol. 142, 232-236, doi:10.1016/j.clim.2011.12.013 (2012).
29. Horvath, K.M., et al. Nasal lavage natural killer cell function is suppressed in smokers after live attenuated influenza virus. Respir. Res. 12, 102, doi:10.1186/1465-9921-12-102 (2011).
30. Noah, T.L., et al. Tobacco smoke exposure and altered nasal responses to live attenuated influenza virus. Environ. Health Perspect. 119, 78-83, doi:10.1289/ehp.1002258 (2011).