Determination of the molecular weight of poly(ethylene glycol) in biological samples by reversed-phase LC-MS with in-source fragmentation

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 12, 2013, Pages 4283-4287

  Warrack, Bethanne M ^a   Redding, Brian P ^b   Chen, Guodong ^a   Bolgar, Mark S ^c  

^a Pharmaceutical Candidate Optimization Bioanalytical and Discovery Analytical Sciences   (United States)

^b Spectrix Analytical Services   (United States)

^c Analytical and Bioanalytical Development   (United States)

Author keywords

In source collision induced dissociation; LC MS; Mass spectrometry; PEG; Poly(ethylene glycol); Selected reactionmonitoring

Indexed keywords

ALIPHATIC COMPOUNDS; DISSOCIATION; DRUG PRODUCTS; ETHYLENE GLYCOL; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; POLYETHYLENE GLYCOLS; POLYOLS; PROTEINS;

CHROMATOGRAPHIC SEPARATIONS; COLLISION INDUCED DISSOCIATION; IN-SOURCE COLLISION-INDUCED DISSOCIATIONS; LC-MS; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; SELECTED ION MONITORING; SELECTED REACTIONMONITORING; STRAIGHTFORWARD STRATEGY;

MOLECULAR WEIGHT;

MACROGOL DERIVATIVE;

ANIMAL; ARTICLE; CHEMISTRY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR WEIGHT; MOUSE; REVERSED PHASE LIQUID CHROMATOGRAPHY; SERUM;

ANIMALS; CHROMATOGRAPHY, REVERSE-PHASE; MASS SPECTROMETRY; MICE; MOLECULAR WEIGHT; POLYETHYLENE GLYCOLS; SERUM;

EID: 84886818232     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-013-6795-3     Document Type: Article

References (17)

1. Kou D, Manius G, Zhan S, Chokshi HP (2009) Size exclusion chromatography with Corona charged aerosol detector for the analysis of polyethylene glycol polymer. J Chromatogr A 1216(28):5424-5428. doi:10.1016/j.chroma.2009.05.043
2. Elliott VL, Edge GT, PhelanMM, Lian LY,Webster R, Finn RF, Park BK, Kitteringham NR (2012) Evidence for metabolic cleavage of a PEGylated protein in vivo using multiple analytical methodologies. Mol Pharm 9(5):1291-1301. doi:10.1021/mp200587m
3. Foser S, Schacher A, Weyer KA, Brugger D, Dietel E, Marti S, Schreitmuller T (2003) Isolation, structural characterization, and antiviral activity of positional isomers of monopegylated interferon alpha-2a (PEGASYS). Protein Expr Purif 30(1):78-87
4. Lee H, Park TG (2003) A novel method for identifying PEGylation sites of protein using biotinylated PEG derivatives. J Pharm Sci 92(1):97-103. doi:10.1002/jps.10270
5. LeeKC, Moon SC, ParkMO, Lee JT,Na DH,Yoo SD, LeeHS, DeLuca PP (1999) Isolation, characterization, and stability of positional isomers of mono-PEGylated salmon calcitonins. Pharm Res 16(6):813-818
6. Seyfried BK, Siekmann J, Belgacem O, Wenzel RJ, Turecek PL, Allmaier G (2010) MALDI linear TOF mass spectrometry of PEGylated (glyco)proteins. J Mass Spectrom 45(6):612-617. doi:10.1002/jms.1746
7. Yoo C, Suckau D, Sauerland V, Ronk M, Ma M (2009) Toward top-down determination of PEGylation site using MALDI insource decay MS analysis. J Am Soc Mass Spectrom 20(2):326-333. doi:10.1016/j.jasms.2008.10.013
8. Li H, Rose MJ, Holder JR, Wright M, Miranda LP, James CA (2011) Direct quantitative analysis of a 20 kDa PEGylated human calcitonin gene peptide antagonist in cynomolgus monkey serum using in-source CID and UPLC-MS/MS. J Am Soc Mass Spectrom 22(9):1660-1667. doi:10.1007/s13361-011-0180-2
9. Fekete S, Ganzler K, Fekete J (2010) Simultaneous determination of polysorbate 20 and unbound polyethylene-glycol in protein solutions using new core-shell reversed phase column and condensation nucleation light scattering detection. J Chromatogr A 1217(40):6258-6266. doi:10.1016/j.chroma.2010.08.028
10. Tang L, Jariwala F, Bolgar M, Lloyd DK (2012) Separation and detection of bis-maleimide-polyethylene glycol and monomaleimide-polyethylene glycol by reversed-phase high pressure liquid chromatography. J Chromatogr A 1246:117-122. doi:10.1016/j.chroma.2012.03.044
11. Bovey FA, Mirau PA (1996) NMR of polymers. Academic Press, San Diego
12. Han J, Danell RM, Patel JR, Gumerov DR, Scarlett CO, Speir JP, Parker CE, Rusyn I, Zeisel S, Borchers CH (2008) Towards highthroughput metabolomics using ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometry. Metabolomics 4(2):128-140. doi:10.1007/s11306-008-0104-8
13. Hood BL, Zhou M, Chan KC, Lucas DA, Kim GJ, Issaq HJ, Veenstra TD, Conrads TP (2005) Investigation of the mouse serum proteome. J Proteome Res 4(5):1561-1568. doi:10.1021/pr050107r
14. Bruce SJ, Jonsson P, Antti H, Cloarec O, Trygg J, Marklund SL, Moritz T (2008) Evaluation of a protocol for metabolic profiling studies on human blood plasma by combined ultra-performance liquid chromatography/mass spectrometry: from extraction to data analysis. Anal Biochem 372(2):237-249. doi:10.1016/j.ab.2007.09.037
15. Huang L, Gough PC, Defelippis MR (2009) Characterization of poly(ethylene glycol) and PEGylated products by LC/MS with postcolumn addition of amines. Anal Chem 81(2):567-577. doi:10.1021/ac801711u
16. Stadalius MA, Gold HS, Snyder LR (1985) Optimization model for the gradient elution separation of peptide mixtures by reversedphase high-performance liquid chromatography. Verification of band width relationships for acetonitrile-water mobile phases. J Chromatogr 327:27-45. doi:10.1016/s0021-9673(01)81636-8
17. Stadalius MA, Quarry MA, Snyder LR (1985) Optimization model for the gradient elution separation of peptide mixtures by reversedphase high-performance liquid chromatography. Application to method development and the choice of column configuration. J Chromatogr 327:93-113. doi:10.1016/s0021-9673(01)81640-x