Direct quantitative analysis of a 20 kDa PEGylated human calcitonin gene peptide antagonist in cynomolgus monkey serum using in-source CID and UPLC-MS/MS

Journal of the American Society for Mass Spectrometry

Volumn 22, Issue 9, 2011, Pages 1660-1667

  Li, Hongyan ^a   Rose, Mark J ^a   Holder, Jerry Ryan ^a   Wright, Marie ^a   Miranda, Les P ^a   James, Christopher A ^a  

^a AMGEN INC   (United States)

Author keywords

CGRP peptide; LC MS MS; PEGylated peptide; Peptide bioanalysis; Quantitation; Therapeutic peptide

Indexed keywords

CGRP PEPTIDE; LC-MS/MS; PEGYLATED PEPTIDE; QUANTITATION; THERAPEUTIC PEPTIDES;

AMINO ACIDS; BODY FLUIDS; EXTRACTION; GENE THERAPY; GLYCOLS; PHARMACOKINETICS; POLYDISPERSITY; POLYETHYLENE GLYCOLS; TISSUE;

PEPTIDES;

PEGYLATED CALCITONIN GENE RELATED PEPTIDE[CITRULLINE,CITRULLINE]; PEPTIDE DERIVATIVE; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL EXPERIMENT; ARTICLE; CALIBRATION; COLLISIONALLY ACTIVATED DISSOCIATION; DRUG BLOOD LEVEL; DRUG ELIMINATION; DRUG HALF LIFE; DRUG ISOLATION; HAPLORHINI; IN SOURCE COLLISIONALLY ACTIVATED DISSOCIATION; LIMIT OF QUANTITATION; MASS SPECTROMETRY; MAXIMUM PLASMA CONCENTRATION; NONHUMAN; QUANTITATIVE ANALYSIS; SINGLE DRUG DOSE; SOLID PHASE EXTRACTION; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; CALCITONIN GENE-RELATED PEPTIDE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; LEAST-SQUARES ANALYSIS; MACACA FASCICULARIS; MOLECULAR SEQUENCE DATA; POLYETHYLENE GLYCOLS; RECEPTORS, CALCITONIN GENE-RELATED PEPTIDE; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

MACACA FASCICULARIS;

EID: 80052689159     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-011-0180-2     Document Type: Article

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