Free light chain content in culture media reflects recombinant monoclonal antibody productivity and quality

Biotechnology Progress

Volumn 29, Issue 5, 2013, Pages 1131-1139

  Bhoskar, Prachi ^a   Belongia, Brett ^b   Smith, Robert ^c   Yoon, Seongkyu ^d   Carter, Tyler ^a   Xu, Jin ^a  

^a University of Massachusetts Lowell   (United States)

^b Bioreactor Process Development   (United States)

^c EMD Millipore   (United States)

^d University of Massachusetts   (United States)

Author keywords

Antibody aggregation; Antibody productivity; Cell culture optimization; Clone selection; Free light chains

Indexed keywords

ANTIBODY AGGREGATION; CELL CULTURE OPTIMIZATION; CELL CULTURE PROCESS; CLONE SELECTION; FREE LIGHT CHAINS; MONOCLONAL ANTIBODIES (MABS); PROCESS DEVELOPMENT; RECOMBINANT MONOCLONAL ANTIBODY;

CHAINS; CLONING; MONOCLONAL ANTIBODIES; OPTIMIZATION; PRODUCTIVITY;

CELL CULTURE;

CULTURE MEDIUM; IMMUNOGLOBULIN G; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL; BIOSYNTHESIS; CELL SURVIVAL; CHEMISTRY; CHO CELL LINE; CRICETULUS; CULTURE MEDIUM; GENE EXPRESSION REGULATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN FOLDING;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL SURVIVAL; CHO CELLS; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; CRICETULUS; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION; IMMUNOGLOBULIN G; IMMUNOGLOBULIN LIGHT CHAINS; MASS SPECTROMETRY; PROTEIN FOLDING; RECOMBINANT PROTEINS;

EID: 84886746934     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1767     Document Type: Article

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