Amplicon Sequencing for Marker Discovery

Molecular Markers in Plants

Volumn , Issue , 2012, Pages 35-56

  Sexton, Timothy R ^a   Shapter, Frances M ^b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b SOUTHERN CROSS UNIVERSITY   (Australia)

Author keywords

Amplicon sequencing for discovery of SNPs and InDels; Amplicon sequencing for marker discovery; Amplicon based MPS and challenge, in uneven coverage rectification; End bias and MPS coverage, across amplicon fragment sizes; First generation (Sanger) sequencing, MPS in bypassing cloning; Insufficient coverage, as concerns in pooled sample MPS; Labeling genotypes with barcodes prior MPS, to its full capacity; MPS platforms, amplicon sequencing, cost effective in high SNP; Pooling genotypes without barcoding, before amplification and MPS; SESAME and reads from pooled barcoded libraries using MPS

Indexed keywords

EID: 84886329488     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781118473023.ch3     Document Type: Chapter

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