A capillary electrophoresis-based enzyme assay for kinetics and inhibition studies of carbonic anhydrase

Analytical Biochemistry

Volumn 444, Issue 1, 2014, Pages 16-21

  Iqbal, Shoaib ^a   Nisar Ur Rahman, ^a   Iqbal, Jamshed ^a  

^a Tobe Camp University Road Abbottabad   (Pakistan)

Author keywords

Capillary electrophoresis; Carbonic anhydrase; Enzyme kinetics; Inhibition assay

Indexed keywords

CAPILLARY ELECTROPHORESIS; CARBONIC ANHYDRASE; ENZYME KINETICS; FUSED SILICA; KINETICS; MAMMALS; SODIUM BORATE;

BOVINE CARBONIC ANHYDRASE; CAPILLARY TEMPERATURE; FUSED-SILICA CAPILLARIES; INHIBITION ASSAYS; INHIBITION CONSTANTS; MINIMAL SAMPLE VOLUME; P-NITROPHENYL ACETATE; PRECISE MEASUREMENTS;

ENZYME INHIBITION;

4 NITROPHENOLATE; 4 NITROPHENYL ACETATE; 4 NITROPHENYL PHOSPHATE; BORATE SODIUM; BUFFER; CARBONATE DEHYDRATASE; SILICON DIOXIDE; UNCLASSIFIED DRUG; URIDINE; CARBONATE DEHYDRATASE II; CARBONATE DEHYDRATASE INHIBITOR; FUROSEMIDE;

ACCURACY; ARTICLE; CAPILLARY; CAPILLARY ZONE ELECTROPHORESIS; CONTROLLED STUDY; COW; ENZYME ASSAY; ENZYME INHIBITION; ENZYME KINETICS; PH; PRESSURE; PRIORITY JOURNAL; SAMPLE SIZE; STANDARD; TEMPERATURE; ANIMAL; ANTAGONISTS AND INHIBITORS; BOVINAE; CAPILLARY ELECTROPHORESIS; CHEMISTRY; DOSE RESPONSE; KINETICS; METABOLISM; PROCEDURES; STRUCTURE ACTIVITY RELATION;

BOVINAE;

ANIMALS; CARBONIC ANHYDRASE II; CARBONIC ANHYDRASE INHIBITORS; CATTLE; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, CAPILLARY; ENZYME ASSAYS; FUROSEMIDE; KINETICS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84886134412     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.09.018     Document Type: Article

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