Capillary electrophoresis-based nanoscale assays for monitoring ecto-5′-nucleotidase activity and inhibition in preparations of recombinant enzyme and melanoma cell membranes

Analytical Biochemistry

Volumn 373, Issue 1, 2008, Pages 129-140

  Iqbal, Jamshed ^a   Jirovsky, David ^b   Lee, Sang Yong ^a   Zimmermann, Herbert ^b   Müller, Christa E ^a  

^a UNIVERSITY OF BONN   (Germany)

^b GOETHE UNIVERSITY   (Germany)

Author keywords

Adenosine 5 monophosphate; Capillary electrophoresis; CD73; Ecto 5 nucleotidase assay; Electrophoretically mediated microanalysis; Melanoma cells; Michaelis Menten analysis

Indexed keywords

BIOMOLECULES; CAPILLARY ELECTROPHORESIS; CHLORINE COMPOUNDS; CYTOLOGY; DERMATOLOGY; ENZYME KINETICS; FUSED SILICA; MAGNESIUM COMPOUNDS; ONCOLOGY; SODIUM BORATE; SUBSTRATES;

CD73; FUSED-SILICA CAPILLARIES; MELANOMA CELLS; MEMBRANE PREPARATION; MICHAELIS-MENTEN; MONOPHOSPHATES; RECOMBINANT ENZYMES; SANDWICH TECHNIQUE;

ENZYME INHIBITION;

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; BORATE SODIUM; CALCIUM CHLORIDE; ECTO 5' NUCLEOTIDASE; ENZYME INHIBITOR; MAGNESIUM CHLORIDE; RECOMBINANT ENZYME;

ARTICLE; CAPILLARY ELECTROPHORESIS; CELL MEMBRANE; CONTROLLED STUDY; ELECTRIC POTENTIAL; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; HYDRODYNAMICS; MELANOMA CELL; NANOCHEMISTRY; NONHUMAN; PRIORITY JOURNAL; RAT;

RATTUS;

EID: 37049009485     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.09.028     Document Type: Article

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