Detection of G Protein-Coupled Receptor (GPCR) Dimerization by Coimmunoprecipitation

Methods in Cell Biology

Volumn 117, Issue , 2013, Pages 323-340

  Skieterska, Kamila ^a   Duchou, Jolien ^a   Lintermans, Béatrice ^a   Van Craenenbroeck, Kathleen ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Coimmunoprecipitation; GPCR oligomerization; HEK293T; Immunoblotting; Overexpression; RIPA buffer

Indexed keywords

DETERGENT; DODECYL SULFATE SODIUM; DOPAMINE 4 RECEPTOR; ISOPROTEIN; UREA;

ARTICLE; CHEMISTRY; COIMMUNOPRECIPITATION; GENE EXPRESSION; GENETIC TRANSFECTION; GENETICS; GLYCOSYLATION; GPCR OLIGOMERIZATION; HEK293 CELL LINE; HEK293T; HUMAN; IMMUNOBLOTTING; IMMUNOPRECIPITATION; KINETICS; METABOLISM; METHODOLOGY; OVEREXPRESSION; PLASMID; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MULTIMERIZATION; RIPA BUFFER; SIGNAL TRANSDUCTION;

COIMMUNOPRECIPITATION; GPCR OLIGOMERIZATION; HEK293T; IMMUNOBLOTTING; OVEREXPRESSION; RIPA BUFFER;

DETERGENTS; GENE EXPRESSION; GLYCOSYLATION; HEK293 CELLS; HUMANS; IMMUNOPRECIPITATION; KINETICS; PLASMIDS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN ISOFORMS; PROTEIN MULTIMERIZATION; RECEPTORS, DOPAMINE D4; SIGNAL TRANSDUCTION; SODIUM DODECYL SULFATE; TRANSFECTION; UREA;

EID: 84885937123     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-408143-7.00017-7     Document Type: Chapter

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