Biosensors Based on Conducting Electroactive Polymers

Conjugated Polymers: Processing and Applications

Volumn 2, Issue , 2007, Pages 12-1-12-45

  Guiseppi Elie, Anthony ^a,b   Brahim, Sean ^c   Wilson, Ann M ^b  

^a AMRL   (United States)

^b ABTECH Scientific Inc   (United States)

^c CLEMSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84885549231     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (222)

1. Castillo, J., S. Gaspar, S. Leth, M. Niculescu, A. Mortari, I. Bontidean, V. Soukharev, S.A. Dorneanu, A.D. Ryabov, and E. Csoregi. 2004. Biosensors for life quality: Design, development and applications. Sens Actuators B 102:179.
2. Hofstadler, S.A., R. Sampath, L.B. Blyn, M.W. Eshoo, T.A. Hall, Y. Jiang, J.J. Drader, J.C. Hannis, K.A. Sannes-Lowery, L.L. Cummins, B. Libby, D.J. Walcott, A. Schink, C. Massire, R. Ranken, J. Gutierrez, S. Manalili, C. Ivy, R. Melton, H. Levene, G. Barrett-Wilt, F. Li, V. Zapp, N. White, V. Samant, J.A. McNeil, D. Knize, D. Robbins, K. Rudnick, A. Desai, E. Moradi, and D.J. Ecker. 2005. TIGER: The universal biosensor. Int J Mass Spectrom 242:23.
3. x. J Chem Soc Chem Commun 578.
4. Chiang, C.K., C.R. Fincher Jr., Y.W. Park, A.J. Heeger, H. Shirakawa, E.J. Louis, S.C. Gau, and A.G. MacDiarmid. 1977. Electrical conductivity in doped polyacetylene. Phys Rev Lett 39 (17):1098.
5. Seeger, K., W.D. Gill, T.C. Clarke, and G.B. Street. 1978. Conductivity and hall effect measurements in doped polyacetylene. Solid State Commun 28 (10):873.
6. 5 doped polyacetylene. Solid State Commun 31 (5):355.
7. Malmros, M.K. 1984. Analytical device having semiconductive organic polymeric element associated with analyte binding substance. US Patent 4,444,892.
8. (a) Lofton, E.P., J.W. Thackeray, and M.S. Wrighton. 1986. Amplification of electrical signals with molecule-based transistors: Power amplification up to a kilohertz frequency and factors limiting higher frequency operation. J Phys Chem 90:6080.
9. Wrighton, M.S., H.S. White Jr., and J.W. Thackeray. 1988. Molecule-based microelectronic devices. US Patent 4,717,673.
10. Wrighton, M.S., H.S. White Jr., and G.P. Kittlesen. 1988. Molecule-based microelectronic devices. US Patent 4,721,601.
11. Wrighton, M.S., H.S. White Jr., and G.P. Kittlesen. 1990. Molecule-based microelectronic devices, US Patent 4,895,705.
12. Wrighton, M.S., H.S. White Jr., and G.P. Kittlesen. 1991. Molecule-based microelectronic devices. US Patent 5,034,192.
13. Umana, M., and J. Waller. 1986. Protein-modified electrodes. The glucose oxidase=polypyrrole system. Anal Chem 58:2979.
14. Ivaska, A. 1991. Analytical applications of conducting polymers. Electroanalysis 3:247.
15. Chidsey, C.E.D., and R.W. Murray. 1986. Electroactive polymers and macromolecular electronics. Science 231:25.
16. Hillman, R.A. 1987. Reactions and applications of polymer modified electrodes. In Electrochemical science and technology of polymers-1, Ed. R.G. Linford, 241. New York: Elsevier.
17. Guiseppi-Elie, A. 1983. Synthesis and characterization of polyacetylene: 1. Stability of doped polyacetylene: 2. Surface chemistry of polyacetylene. Sc.D. Thesis in Materials Science and Engineering, Massachusetts.
18. Guiseppi-Elie, A., and G.E. Wnek. 1990. Aqueous reactivity of poly-acetylene: pH dependence. J Phys Chem 97 (7):3192.
19. Heller, A. 1990. Electrical wiring of redox enzymes. Acc Chem Res 23:128.
20. Lewis, T.W., G.G. Wallace, and M.R. Smyth. 1999. Electrofunctional polymers: Their role in the development of new analytical systems. Analyst 124:213.
21. Santhanam, K.S.V. 1998. Conducting polymers for biosensors: Rationale based on models. Pure Appl Chem 70:1259.
22. Kranz, C., H. Wohlschlager, H.L. Schmidt, and W. Schuhmann. 1998. Controlled electrochemical preparation of amperometric biosensor based on conducting polymer multilayers. Electroanalysis 10:546.
23. Trojanowicz, M., T.K. vel Krawczyk, and P.W. Alexander. 1997. Organic conductings as active materials in electrochemical chemosensors and biosensors. Chem Anal 42:199.
24. Adeloju, S.B., and G.G. Wallace. 1996. Conducting polymers and the bioanalytical sciences. Analyst 121:699.
25. Trojanowicz, M., and T.K. vel Krawczyk. 1995. Electrochemical biosensors based on enzymes immobilized in electropolymerized films. Mikrochim Acta 121:167.
26. Contractor, A.Q., T.N. Sureshkumar, R. Narayanan, S. Sukeerthi, R. Lal, and R.S. Srinivasa. 1994. Conducting polymer based biosensors. Electrochim Acta 39:1321.
27. Bartlett, P.N., and P.R. Birkin. 1993. The application of conducting polymers in biosensors. Synth Met 61:15.
28. Schuhmann, W. 1995. Conducting polymers and their application in amperometric biosensors. Mikrochim Acta 121:1.
29. Boyle, A., E.M. Genies, and M. Lapkowski. 1989. Application of electronic conducting polymers as sensors: Polyaniline in the solid state for detection of solvent vapors and polypyrrole for detection of biological ions in solutions. Synth Met 28:C769.
30. Gregg, B.A., and A. Heller. 1990. Cross-linked redox gels containing glucose oxidase for ampero-metric biosensor applications. Anal Chem 62:258.
31. Lidzey, D.G., D.C. Bradley, S.F. Alvarado, and P.F. Seidler. 1997. Electroluminescence in polymer films. Nature 386:135.
32. Shen, Z., P.E. Burrows, V. Bulovic´, S.R. Forrest, and M.E. Thompson. 1997. Three-color, tunable, organic light-emitting devices. Science 276:2009.
33. Kaneto, K., and M. Kaneko. 2002. Handbook of polymers in electronics, Ed. B.D. Malhotra, 255. Shawbury, UK: Rapra Technology Ltd., chap. 8.
34. Sharma, A.L., M. Gerard, R. Singhal, S. Annapoorni, and B.D. Malhotra. 2001. Synthesis and characterization of fluoro-substituted polyaniline. Appl Biochem Biotechnol 96:155.
35. Sharma, A.L., V. Saxena, S. Annapoorni, and B.D. Malhotra. 2001. Synthesis and characterization of a copolymer: Poly(aniline-co-fluoroaniline). J Appl Polym Sci 81:1460.
36. Chaubey, A., K.K. Pandey, V.S. Singh, and B.D. Malhotra. 2000. Co-immobilization of lactate oxidase and lactate dehydrogenase on conducting polyaniline films. Anal Chim Acta 407:97.
37. Paul, E.W., A.J. Ricco, and M.S. Wrighton. 1985. Resistance of polyaniline films as a function of electrochemical potential and the fabrication of polyaniline-based microelectronic devices. J Phys Chem 89:1441.
38. Bidan, G. 1992. Electroconducting conjugated polymers: New sensitive matrices to build up chemical or electrochemical sensors. A review. Sens Actuators B Chem 6 (1–3):45.
39. Centonze, D., C. Malitesta, F. Palmisano, and P.G. Zambonin. 1994. Permeation of solutes through an electropolymerized ultra thin poly-o-phenylenediamine film used as an enzyme-entrapped membrane. Electroanalysis 6:423.
40. Chien, J.C.W. 1984. Polyacetyene: Chemistry physics and materials science. New York: Academic Press.
41. MacDiarmid, A.G., and A.J. Epstein. 1995. Secondary doping in polyaniline. Synth Met 69:85.
42. MacDiarmid, A.G. 1997. Polyaniline and polypyrrole: Where are we headed? Synth Met 84:27.
43. Kittlesen, G.P., H.S. White, and M.S. Wrighton. 1984. Chemical derivativization of microelectrode arrays by oxidation of pyrrole and N-methylpyrrole: Fabrication of molecule-based electronic devices. J Am Chem Soc 106:7389.
44. Guiseppi-Elie, A., and A.M. Wilson. 1995. Electroconductive polymer thin films with internal bioactive moieties for biosensor applications. Proc Am Chem Soc Div Polym Mater: Sci Eng 72:404.
45. Malmros, M.K., and A. Guiseppi-Elie. 1987. Sensor applications of electroactive polymers. MIT Summer Session Program, Cambridge, Massachusetts, July 22.
46. Swager, T.M. 1998. The molecular wire approach to sensory signal amplification. Acc Chem Res 31:201.
47. Guiseppi-Elie, A., and L. Lingerfelt. 2005. Impedimetric detection of DNA hybridization: Towards near patient DNA diagnostics. In Current topics in chemistry, Ed. Christine Withmann. Germany: Springer Verlag.
48. Teasdale, P.R., and G.G. Wallace. 1993. Molecular recognition using conducting polymers: Basis of an electrochemical sensing technology. Analyst 118:329.
49. Sargent, A., and O.A. Sadik. 1999. Monitoring antibody–antigen reactions at conducting polymer-based immunosensors using impedance spectroscopy. Electrochim Acta 44:4667.
50. Georghe, M., C. Lei, and A. Guiseppi-Elie. 2000. Low density arrays of DNA-doped polypyrrole. Proc ACS PMSE Preprints 83:550.
51. Lei, C., M. Georghe, and A. Guiseppi-Elie. 2000. DNA immobilization and bioelectronic detection based on conducting polymers. Proc ACS PMSE Preprints 83:552.
52. Geoffrey, L., P. Peter, and P. Vadgama. 2001. Electrochemical impedance spectroscopy as a platform for reagentless bioaffinity sensing. Sens Actuators B Chem 78:249.
53. Ouerghi, O., A. Touhami, N. Jaffrezic-Renault, C. Martelet, H. Ben Ouada, and S. Cosnier. 2002. Impedimetric immunosensor using avidin–biotin for antibody immobilization. Bioelectrochemistry 56:131.
54. Ramanathan, K., M.K. Ram, B.D. Malhotra, and A.S.N. Murthy. 1995. Application of polyaniline Langmuir–Blodgett films as a glucose biosensor. Mater Sci Eng C 3:159.
55. Lobo, M.J., A.J. Miranda, and P. Tunon. 1997. Amperometric biosensors based on NAD(P)-dependent dehydrogenase enzymes. Electroanalysis 9:191.
56. Willner, I., and E. Katz. 2000. Integration of layered redox proteins and conductive supports for bioelectronic applications. Ang Chem Int Ed 39:1180.
57. Foulds, N.C., and C.R. Lowe. 1986. Enzyme entrapment in electrically conducting polymers, immobilization of glucose-oxidase in polypyrrole and its application in amperometric glucose sensors. J Chem Soc Faraday Trans 1 82:1259.
58. Umana, M., and J. Waller. 1986. Protein-modified electrodes. The glucose oxidase=polypyrrole system. Anal Chem 58:2979.
59. Sangodkar, H., S. Sukeerthi, R.S. Srinivasa, R. Lal, and A.Q. Contractor. 1996. A biosensor array based on polyaniline. Anal Chem 68:779.
60. Garjonyte, R., and A. Malinauskas. 2000. Amperometric glucose biosensors based on Prussian Blue—and polyaniline—glucose oxidase modified electrodes. Biosens Bioelectron 15 (9–10):445.
61. J.C. Vidal, E. Garcia, and J.R. Castillo. 1999. In situ preparation of overoxidized PPy=oPPD bilayer biosensors for the determination of glucose and cholesterol in serum. Sens Actuators B Chem 57 (1–3):219.
62. Guerrieri, A., G.E. De Benedetto, F. Palmisano, and P.G. Zambonin. 1998. Electrosynthesized nonconducting polymers as perm selective membranes in amperometric enzyme electrodes: A glucose biosensor based on a co-crosslinked glucose oxidase=overoxidized polypyrrole bilayer. Biosens Bioelectron 13:103.
63. Palmisano, F., D. Centonze, A. Guerrieri, and P.G. Zambonin. 1993. An interference-free biosensor based on glucose oxidase electrochemically immobilized in a non-conducting poly(pyrrole) film for continuous subcutaneous monitoring of glucose through microdialysis sampling. Biosens Bioelectron 8:393.
64. Shin, M.-C., H.C. Yoon, and H.-S. Kim. 1996. In situ biochemical reduction of interference in an amperometric biosensor with a novel heterobilayer configuration of polypyrrole=glucose oxidase=horseradish peroxidase. Anal Chim Acta 329 (3):223.
65. Tian, F., and G. Zhu. 2002. Bienzymatic amperometric biosensor for glucose based on polypyrrole=ceramic carbon as electrode material. Anal Chim Acta 451:251.
66. Rohde, E., E. Dempsey, M.R. Smyth, J.G. Vos, and H. Emons. 1993. Development of a flow-through electrochemical detector for glucose based on a glucose oxidase-modified microelectrode incorporating redox and conducting polymer materials. Anal Chim Acta 278:5.
67. Schuhmann, W. 1991. Amperometric substrate determination in flow-injection systems with polypyrrole-enzyme electrodes. Sens Actuators B 4:41.
68. Liu, X., K.G. Neoh, L. Cen, and E.T. Kang. 2004. Enzymatic activity of glucose oxidase covalently wired via viologen to electrically conductive polypyrrole films. Biosens Bioelectron 19:823.
69. Sung, W.J., and Y.H. Bae. 2003. A glucose oxidase electrode based on polypyrrole with poly-anion=PEG=enzyme conjugate dopant. Biosens Bioelectron 18:1231.
70. Wang, J., and M. Musameh. 2005. Carbon-nanotubes doped polypyrrole glucose biosensor. Anal Chim Acta 539 (1–2):209.
71. Wang, J., J. Dai, and T. Yarlagadda. 2005. Carbon nanotube-conducting-polymer composite nanowires. Langmuir 21 (1):9.
72. Wang, J., N.V. Myung, M. Yun, H.G. Monbouquette. 2005. Glucose oxidase entrapped in poly-pyrrole on high-surface-area Pt electrodes: A model platform for sensitive electroenzymatic biosensors. J Electroanal Chem 575:139.
73. Yasuzawa, M., T. Matsuki, H. Mitsui, A. Kunugi, and T. Nakaya. 2000. Properties of glucose sensors prepared by the electropolymerization of pyrroles containing phosphatidylcholine (II). Sens Actuators B 66:25.
74. Vincent, J.L. 1998. Lactate and biochemical indexes of oxygenation. In Principles and practice of intensive care monitoring, Ed. M.J. Tobin, McGraw-Hill Inc., New York.
75. Ward, K.R., R.R. Ivatury, and R.W. Barbee. 2001. Endpoints of resuscitation for the victim of trauma. J Intensive Care Med 16:55.
76. Rudel, U., O. Geschke, and K. Cammann. 2005. Entrapment of enzymes in electropolymers for biosensors and graphite felt based flow-through enzyme reactors. Electroanalysis 8 (12):1135.
77. Chaubey, A., K.K. Pande, M.K. Pandey, and V.S. Singh. 2001. Signal amplification by substrate recycling on polyaniline=lactate oxidase=lactate dehydrogenase bienzyme electrodes. App Biochem Biotechnol 96 (1–3):239.
78. Chaubey, A., M. Gerard, R. Singhal, V.S. Singh, and B.D. Malhotra. 2001. Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole–polyvinylsulphonate composite films for application to lactate biosensors. Electrochim Acta 46 (5):723.
79. Guiseppi-Elie, A., S. Brahim, G. Slaughter, and K. Ward. 2005. Design of a subcutaneous implan-table biochip for monitoring of glucose and lactate. IEEE Sens J 5 (3):345.
80. Bobacka, J., A. Ivaska, and A. Lewenstam. 2003. Potentiometric ion sensors based on conducting polymers. Electroanalysis 15 (5–6):366.
81. Adeloju, S.B., and A.N. Moline. 2001. Fabrication of ultra-thin polypyrrole–glucose oxidase film from supporting electrolyte-free monomer solution for potentiometric biosensing of glucose. Biosens Bioelectron 16:133.
82. Trojanowicz, M., and M.L. Hitchman. 1996. A potentiometric polypyrrole-based glucose biosensor. Electroanalysis 8:263.
83. Karyakina, E.E., L.V. Neftiakova, and A.A. Karyakin. 1994. A novel potentiometric glucose biosensor based on polyaniline semiconductor film. Anal Lett 27:2871.
84. Komaba, S., M. Seyama, T. Momma, and T. Osaka. 1996. Potentiometric biosensor for urea based on electropolymerized electroinactive polypyrrole. Electrochim Acta 42 (3):383.
85. Koezuka, H., A. Tsumura, and Y. Ando. 1987. Field-effect transistor with polythiophene thin film. Synth Met 18:699.
86. Lee, M.S., H.S. Kang, H.S. Kang, J. Joo, A.J. Epstein, and J.Y. Lee. 2005. Flexible all-polymer field effect transistors with optical transparency using electrically conducting polymers. Thin Solid Films 477:169.
87. Hatfield, J.V., J.A. Covington, and J.W. Gardner. 2000. GasFETs incorporating conducting polymers as gate materials. Sens Actuators B Chem 65:253.
88. Tada, K., H. Harada, M. Onoda, H. Nakayama, and K. Yoshino. 1999. Percolation in carrier transport in FET with dye doped conducting polymers. Synth Met 102:981.
89. Drury, C.J., C.M.J. Mutsaers, C.M. Hart, M. Matters, and D.M. de Leeuw. 1998. Low-cost all-polymer integrated circuits. Appl Phys Lett 73:108.
90. Nicholas, J.L., J. Pinto, and A.G. MacDiarmid. 2002. Apparent dependence of conductivity of a conducting polymer on an electric field in a field effect transistor configuration. J Appl Phys 92:6033.
91. Nilsson, D., M. Chen, T. Kugler, T. Remonen, M. Armgarth, and M. Berggren. 2002. Bistable and dynamic modulation in organic electrochemical transistors. Adv Mater 14:51.
92. Matters, M., D.M. de Leeuw, M.J.C.M. Vissenberg, C.M. Hart, P.T. Herwig, T. Geuns, C.M.J. Mutsaers, and C.J. Drury. 1999. Organic field-effect transistors and all-polymer integrated circuits. Opt Mater 12:189.
93. Rogers, J.A., Z. Bao, and V.R. Raju. 1998. Nonphotolithographic fabrication of organic transistors with micron feature sizes. Appl Phys Lett 72:21.
94. Sheraw, C.D., L. Zhou, J.R. Huang, D.J. Gundlach, T.N. Jackson, M.G. Kane, I.G. Hill, M.S. Hammond, J. Campi, B.K. Grening, J. Francl, and J. West. 2002. Organic thin-film transistor-driven polymer-dispersed liquid crystal displays on flexible polymeric substrates. Appl Phys Lett 80:6.
95. MacDiarmid, A.G. 2001. “Synthetic Metals”: A Novel Role for Organic Polymers (Nobel Lecture). Ang Chem Int Ed Engl 40:2581.
96. Garnier, F. 1996. Thin film transistors based on molecular semiconductors. Pure Appl Chem 68:1455.
97. Harima, Y., T. Eguchi, and K. Yamashita. Enhancement of carrier mobilities in poly(3-methylthio-phene) by an electrochemical doping. Synth Met 95:69.
98. (a) Laquindanum, J.G., H.E. Katz, and A.J. Lovinger. 1998. Synthesis, morphology, and field-effect mobility of anthradithiophenes. J Am Chem Soc 120:664.
99. Dimitrakopoulos, C.D., B.K. Furman, T. Graham, S. Hegde, and S. Purushothaman. 1998. Field-effect transistors comprising molecular beam deposited a-v-di-hexyl-hexathienylene and polymeric insulators. Synth Met 92:47.
100. Klein, D.L., R. Roth, A.K.L. Lim, A.P. Alivisatos, and P.L. McEuen. 1997. A single-electron transistor made from cadmium selenide nanocrystal. Nature (London) 389:699.
101. Harima, Y., Y. Kunugi, K. Yamashita, and M. Shiotani. 2000. Determination of mobilities of charge carriers in electrochemically anion-doped polythiophene film. Chem Phys Lett 317:310.
102. Kunugi, Y., Y. Harima, K. Yamashita, N. Ohta, and S. Ito. 2000. Charge transport in a regioregular poly(3-octylthiophene) film. J Mater Chem 10:2673.
103. Sirringhaus, H., N. Tessler, and R.H. Friend. 1998. Integrated optoelectronic devices based on conjugated polymers. Science 280:1741.
104. Chen, M. 2005. Printed electrochemical devices using conducting polymers as active materials on flexible substrates. Proc IEEE 93 (7):1339.
105. Mu, S., and H. Xue. 1996. Bioelectrochemical characteristics of glucose oxidase immobilized in a polyaniline film. Sens Actuators B Chem 31 (3):139.
106. Kumar, A., A. Chaubey, S.K. Grover, and B.D. Malhotra. 2001. Immobilization of cholesterol oxidase and potassium ferricyanide on dodecylbenzene sulfonate ion-doped polypyrrole film. J Appl Polym Sci 82 (14):3486.
107. Gajovic, N., K. Habermuller, A. Warsinke, W. Schuhmann, and F. W. Scheller. 1999. APyruvateoxidase electrode based on an electrochemically deposited redox polymer. Electroanalysis 11 (18):1377.
108. Milagres, B.G., G.D. Neto, L.T. Kubota, and H. Yamanaka. 1997. A new amperometric biosensor for salicylate based on salicylate hydroxylase immobilized on polypyrrole film doped with hexacyano-ferrate. Anal Chim Acta 347 (1–2):35.
109. Chaubey, A., M. Gerard, R. Singhal, V.S. Singh, and B.D. Malhotra. 2000. Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole–polyvinylsulphonate composite films for application to lactate biosensors. Electrochim Acta 46 (5):723.
110. Mc Ardle, F.A., and K.C. Persaud. 1993. Development of an enzyme-based biosensor for atrazine detection. Analyst 118:419.
111. Trojanowicz, M., O. Geschke, and T. Krawczynski vel Krawczyk. 1995. Biosensors based on oxidases immobilized in various conducting polymers. Sens Actuators B 28 (3):191.
112. Yifei, Y., and M. Shaolin. 1997. Bioelectrochemical responses of the polyaniline horseradish peroxidase electrodes. J Electroanal Chem 432 (1–2):71.
113. Wang, H., and S. Mu. 1999. Bioelectrochemical characteristics of cholesterol oxidase immobilized in a polyaniline film. Sens Actuators B 56 (1–2):22.
114. Kan, J.Q., S.L. Mu, and H. Chen. 1997. Effects of conducting polymers on immobilized galactose oxidase. Synth Met 87 (3):205.
115. Xue, H.G., and S.L. Mu. 1995. Bioelectrochemical response of the polypyrrole xanthine oxidase electrode. J Electroanal Chem 397 (1–2):241.
116. Shaolin, M., K. Jinqing, and Z. Jianbing. 1992. Bioelectrochemical responses of the polyaniline uricase electrode. J Electroanal Chem 334 (1–2):121.
117. Yon Hin, B.F.Y., M. Smolander, T. Crompton, and C.R. Lowe. 1993. Covalent electropoly-merization of glucose oxidase in polypyrrole. Evaluation of methods of pyrrole attachment to glucose oxidase on the performance of electropolymerized glucose sensors. Anal Chem 65:2067.
118. Kojima, K., T. Yamauchi, M. Shimomura, and S. Miyauchi. 1998. Covalent immobilization of glucose oxidase on poly[1-(2-carboxyethyl)pyrrole] film for glucose sensing. Polymer 39:2079.
119. Ramanathan, K., S.S. Pandey, R. Kumar, A. Gulati, A. Surya, N. Murthy, and B.D. Malhotra. 2000. Covalent immobilization of glucose oxidase to poly(O-amino benzoic acid) for application to glucose biosensor. J Appl Polym Sci 78:662.
120. Reiter, S., K. Habermuller, and W. Schuhmann. 2001.Areagentlessglucosebiosensor basedonglucose oxidase entrapped into osmium-complex modified polypyrrole films. Sens Actuators B 79:150.
121. Tian, F., and G. Zhu. 2002. Bienzymatic amperometric biosensor for glucose based on polypyrrole=ceramic carbon as electrode material. Anal Chim Acta 451 (2):251.
122. Garjonyte, R., and A. Malinauskas. 2000. Glucose biosensor based on glucose oxidase immobilized in electropolymerized polypyrrole and poly(o-phenylenediamine) films on a Prussian Blue-modified electrode. Sens Actuators B 63:122.
123. Arjsiriwat, S., M. Tanticharoen, K. Kirtikara, K. Aoki, and M. Somasundrum. 2000. Metal-dispersed conducting polymer-coated electrode used for oxidase-based biosensor. Electrochem Commun 2:441.
124. Vidal J.C., E. Garcia, and J.R. Castillo. 1999. In situ preparation of a cholesterol biosensor: Entrapment of cholesterol oxidase in an overoxidized polypyrrole film electrodeposited in a flow system: Determination of total cholesterol in serum. Anal Chim Acta 385:213.
125. Tatsuma, T., M. Gondaira, and T. Watanabe. 1992. Peroxidase-incorporated polypyrrole membrane electrodes. Anal Chem 64 (10):1183.
126. Uchiyama, S., Y. Hasebe, and M. Tanaka. 1997. L-ascorbate sensor with an immobilized ascorbate oxidase carbon felt prepared by electropolymerization of pyrrole in cucumber juice. Electroanalysis 9:176.
127. Adeloju, S.B., J.N. Barisci, and G.G. Wallace. 1996. Electroimmobilisation of sulphite oxidase into a polypyrrole film and its utilisation for flow amperometric detection of sulphite. Anal Chim Acta 332:145.
128. Xue, H.G., and Z.Q. Shen. 2002. A highly stable biosensor for phenols prepared by immobilizing polyphenol oxidase into polyaniline–polyacrylonitrile composite matrix. Talanta 57 (2):289.
129. Kizilyar, N., U. Akbulut, L. Toppare, M.Y. Ozden, and Y. Yagci. 1999. Immobilization of invertase in conducting polypyrrole=polytetrahydrofuran graft polymer matrices. Synth Met 104:45.
130. Dobay, R., G. Harsanyi, and C. Visy. 1999. Detection of uric acid with a new type of conducting polymer-based enzymatic sensor by bipotentiostatic technique. Anal Chim Acta 385:187.
131. Kranz, C., H. Wohlschlager, H.L Schmidt, and W. Schuhmann. 1998. Controlled electrochemical preparation of amperometric biosensors based on conducting polymer multilayers. Electroanalysis 10 (8):546.
132. Matsue, T., N. Kasai, M. Narumi, and N. Nishizawa. 1991. Electron-transfer from NADH dehy-drogenase to polypyrrole and its applicability to electrochemical oxidation of NADH. J Electroanal Chem 300:111.
133. Haruyama, T., H. Shinohara, Y. Ikariyama, and M. Aizawa. 1993. Modulation of the function of an enzyme immobilized in a conductive polymer by electrochemical changing of the substrate concentration. J Electroanal Chem 347:293.
134. Yabuki, S., F. Mizutani, and M. Asai. 1991. Preparation and characterization of an electroconductive membrane containing glutamate dehydrogenase, NADP, and mediator. Biosens Bioelectron 6:311.
135. Malitesta, C., I. Losito, and G. Zambonin. 1999. Molecularly imprinted electrosynthesized polymers: new materials for biomimetic sensors. Anal Chem 71:1366.
136. Deore, B., Z. Chen, and T. Nagaoka. 1999. Overoxidized polypyrrole with dopant complementary cavities as a new molecularly imprinted polymer matrix. Anal Sci 15:827.
137. Panasyuk, T.L., V.M. Mirsky, S.A. Piletsky, and O.S. Wolfbeis. 1999. Electropolymerized molecu-larly imprinted polymers as receptor layers in capacitive chemical sensors. Anal Chem 71:4609.
138. Liang, H.-J., T.-R. Ling, J.F. Rick, and T.-C. Chou. 2005. Molecularly imprinted electrochemical sensor able to enantroselectively recognize D- and L-tyrosine. Anal Chim Acta 542:83.
139. Blanco-Lopez, M.C., M.J. Lobo-Castanon, A.J. Miranda-Ordieres, and P. Tunon-Blanco. 2004. Electrochemical sensors based on molecularly imprinted polymers. Trends Anal Chem 23:36.
140. Haupt, K., and K. Mosbach. 2000. Molecularly imprinted polymers and their use in biomimetic sensors. Chem Rev 100:2495.
141. Yu, J.C.C., and E.P.C. Lai. 2005. Interaction of ochratoxin A with molecularly imprinted poly-pyrrole film on surface plasmon resonance sensor. React Funct Polym 63:171.
142. Hutchins, R.S., and L.G. Bachas. 1995. Nitrate-selective electrode developed by electrochemically mediated imprinting=doping polypyrrole. Anal Chem 67:1654.
143. Yeh, W.-M., and K.-C. Ho. 2005. Amperometric morphine sensing using a molecularly imprinted polymer-modified electrode. Anal Chim Acta 542:76.
144. Campbell, T.E., A.J. Hodgson, and G.G. Wallace. 1999. Incorporation of erythrocytes into poly-pyrrole to form the basis of a biosensor to screen for rhesus (D) blood groups and rhesus (D) antibodies. Electroanalysis 11 (4):215.
145. Haddour, N., S. Cosnier, and C. Gondran. 2004. Electrogeneration of a biotinylated poly(pyrrole– ruthineum (II)) film for the construction of photoelectrochemical immunosensor. Chem Comm 2472.
146. Brahim, S., A.M. Wilson, D. Narinesingh, E. Iwuoha, and A. Guiseppi-Elie. 2003. Chemical and biological sensors based on electrochemical detection using conducting electroactive Polymers. Microchim Acta 143 (2–3):123.
147. Guiseppi-Elie, A., A.M. Wilson, and A.S. Sujdak. 1998. Electroconductive gels for controlled electrorelease of bioactive peptides. ACS Symp Ser 709, chap. 15, 185.
148. Misoska, V., J. Ding, J.M. Davey, W.E. Price, S.F. Ralph, and G.G. Wallace. 2001. Polypyrrole membranes containing chelating ligands: Synthesis, characterization and transport studies. Polymer 42:8571.
149. Atobe, M., S. Hitose, and T. Nonaka. 1999. Chemistry in centrifugal fields Part I. Electrooxidative polymerization of aniline. Electrochem Commun 1:278.
150. Eftekhari, A. 2004. Enhanced stability and conductivity of polypyrrole film prepared electrochem-ically in the presence of centrifugal forces. Synth Met 142:305.
151. Smela, E., G. Zuccarello, H. Kariis, and B. Liedberg. 1998. Thiol modified pyrrole monomers. Part 1. Synthesis, characterization, and polymerization of 1-(2-thioethyl)pyrrole and 3-(2-thioethyl)-pyrrole. Langmuir 14 (11):2970.
152. Smela, E., H. Kariis, Z.P. Yang, K. Uvdal, G. Zuccarello, and B. Liedberg. 1998. Thiol modified pyrrole monomers. Part 2. As-deposited monolayers of 1-(2-thioethyl)pyrrole and 3-(2-thioethyl)-pyrrole. Langmuir 14 (11):2976.
153. Smela, E., H. Kariis, Z.P. Yang, M. Mecklenburg, and B. Liedberg. 1998. Thiol modified pyrrole monomers. Part 3. Electrochemistry of 1-(2-thioethyl)pyrrole and 3-(2-thioethyl)pyrrole mono-layers in propylene carbonate. Langmuir 14 (11):2984.
154. Smela, E. 1998. Thiol modified pyrrole monomers. Part 4. Electrochemical deposition of poly-pyrrole over 1-(2-thioethyl)pyrrole. Langmuir 14 (11):2996.
155. Idla, K., O. Inganas, and M. Strandberg. 2000. Good adhesion between chemically oxidized titanium and electrochemically deposited polypyrrole. Electrochim Acta 45 (13):2121.
156. Guiseppi-Elie, A., A.M. Wilson, J.M. Tour, T.W. Brockmann, P. Zhang, and D.L. Allara. 1995. Specific immobilization of electropolymerized polypyrrole thin films onto interdigitated micro-sensor electrode arrays. Langmuir 11:1768.
157. Abraham, S., S. Brahim, K. Ishihara, and A. Guiseppi-Elie. 2005. Molecularly engineered p(HEMA)-based hydrogels for implant biochip biocompatibility. Biomaterials 26:4767.
158. Bongiovanni, C., T. Ferri, A. Poscia, M. Varalli, R. Santucci, and A. Desideri. 2001. An electrochemical multienzymatic biosensor for determination of cholesterol. Bioelectrochemistry 54:17.
159. Singh, S., A. Chaubey, and B.D. Malhotra. 2004. Amperometric cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on conducting polypyrrole films. Anal Chim Acta 502 (2):229.
160. Yamato, H., M. Ohawa, and W. Wernet. 1995. A polypyrrole=three enzyme electrode for creatinine detection. Anal Chem 67:2776.
161. Vidal, J.C., E. Garcia, S. Mendez, P. Yarnoz, and J.R. Castillo. 1999. Three approaches to the development of selective bilayer amperometric biosensors for glucose by in situ electropolymeriza-tion. Analyst 124:319.
162. Vidal, J.C., E. Garcia-Ruiz, and J.R. Castillo. 2001. Design of a multilayer cholesterol amperometric biosensor for preparation and use in flow systems. Electroanalysis 13 (3):229.
163. Kranz, C., H. Wohlschager, H.L. Schmidt, and W. Schuhmann. 1998. Controlled electrochemical preparation of amperometric biosensors based on conducting polymer multilayers. Electroanalysis 10 (8):546.
164. Rohde, E., E. Dempsey, M.R. Smyth, J.G. Vos, and H. Emons. 1993. Development of a flow-through electrochemical detector for glucose based on a glucose oxidase-modified microelectrode incorporating redox and conducting polymer materials. Anal Chim Acta 278 (1):5.
165. Brahim, S., D. Narinesingh, and A. Guiseppi-Elie. 2002. Interferent suppression using a novel polypyrrole-containing hydrogel in amperometric enzyme biosensors. Electroanalysis 14 (9):627.
166. Bartlett, P.N., and P.R. Birkin. 1994. A microelectrochemical enzyme transistor responsive to glucose. Anal Chem 66 (9):1552.
167. Bartlett, P.N., Z. Ali, and V. Eastwick-Field. 1992. Electrochemical immobilization of enzymes. Part 4. Co-immobilization of glucose oxidase and ferro=ferricyanide in poly(N-methylpyrrole) films. J Chem Soc Faraday Trans 88:2677.
168. Vidal, J.C., E. Garcia, and J.R. Castillo. 2002. Development of a platinized and ferrocene-mediated cholesterol amperometric biosensor based on electropolymerization of polypyrrole in a flow system. Anal Sci 18 (5):537.
169. Curulli, A., I. Carelli, O. Trischitta, and G. Palleschi. 1997. Enzyme electrode probes obtained by electropolymerization of monomers with PMS and selected dehydrogenase enzymes. Talanta 44 (9):1659.
170. Khan, G.F., E. Kobatake, Y. Ikariyama, and M. Aizawa. 1993. Amperometric biosensor with PQQ enzyme immobilized in a mediator-containing polypyrrole matrix. Anal Chim Acta 281 (3):527.
171. Schuhmann, W. 1995. Electron-transfer pathways in amperometric biosensors. Ferrocene-modified enzymes entrapped in conducting-polymer layers. Biosens Bioelectron 10:181.
172. Habermuller, K., A. Ramanavicius, V. Laurinavicius, and W. Schuhmann. 2000. An oxygen-insensitive reagentless glucose biosensor based on osmium-complex modified polypyrrole. Electro-analysis 12 (17):1383.
173. Chen, J., A.K. Burrell, G.E. Collis, D.L. Officer, G.F. Swiegers, C.O. Too, and G.G. Wallace. 2002. Preparation, characterization and biosensor application of conducting polymers based on ferrocene substituted thiophene and terthiophene. Electrochim Acta 47 (17):2715.
174. Deronzier, A., and J.C. Moutet. 1996. Polypyrrole films containing metal complexes: Syntheses and applications. Coordinat Chem Rev 147:339.
175. Karyakin, A.A., E.E. Karyakina, and H.L. Schmidt. 1999. Electropolymerized azines: A new group of electroactive polymers. Electroanalysis 11 (3):149.
176. Mao, L., and K. Yamamoto. 2000. Glucose and choline on-line biosensors based on electropoly-merized Meldola’s blue. Talanta 51 (1):187.
177. Cosnier, S., D. Fologea, S. Szunerits, and R.S. Marks. 2000. Poly(dicarbazole-N-hydroxysuccini-mide) film: A new polymer for the reagentless grafting of enzymes and redox mediators. Electro-chem Commun 2 (12):827.
178. Situmorang, M., D.B. Hibbert, and J.J. Gooding. 2000. An experimental design study of interferences of clinical relevance of a polytyramine immobilized-enzyme biosensor. Electroanalysis 12 (2):111.
179. Piro, B., V.A. Do, L.A. Le, M. Hedayatullah, and M.C. Pham. 2000. Electrosynthesis of a new enzyme-modified electrode for the amperometric detection of glucose. J Electroanal Chem 486 (2):133.
180. Soloducho, J. 1999. Convenient synthesis of polybispyrrole system. Synth Met 99 (3):181.
181. Jerome, C., V. Geskin, R. Lazzaroni, J.L. Bredas, A. Thibaut, C. Calberg, I. Bodart, M. Mertens, L. Martinot, D.J. Rodrigue Riga, and R. Jerome. 2001. Full-electrochemical preparation of conducting=insulating binary polymer films. Chem Mater 13:1656.
182. Cosnier, S. 1999. Biomolecule immobilization on electrode surfaces by entrapment or attachment to electrochemically polymerized films. A review. Biosens Bioelectron 14 (5):443.
183. Mailley, P. 2001. Inclusion of metal micro-particles into poly(pyrrolylalkylammonium) films containing an enzyme for bioelectrocatalysis: A preliminary study. Talanta 55 (5):1005.
184. Cosnier, S., F. Lambert, and M. Stoytcheva. 2000. A composite clay glucose biosensor based on an electrically connected HRP. Electroanalysis 12 (5):356.
185. Leca, B., R.M. Morelis, and P.R. Coulet. 1994. Influence of phospholipidic microenvironment on the performance of a polypyrrole enzyme electrode. Talanta 41 (6):925.
186. Yabuki, S., F. Mizutani, and M. Asai. 1991. Preparation and characterization of an electroconduc-tive membrane containing glutamate dehydrogenase, NADP, and mediator. Biosens Bioelectron 6 (4):311.
187. Khan, G.F., E. Kobatake, H. Shinohara, Y. Ikariyama, and M. Aizawa. 1992. Molecular interface for an activity controlled enzyme electrode and its application for the determination of fructose. Anal Chem 64 (11):1254.
188. Aizawa, M., T. Haruyama, G.F. Khan, E. Kobatane, and Y. Ikariyama. 1994. Electronically modulated biological functions of molecular interfaced enzymes and living cells. Biosens Bioelectron 9 (9):601.
189. Koopal, C.G.J., and R.J.M. Nolte. 1994. Kinetic study of the performance of third-generation biosensors. Bioelectrochem Bioenerg 33 (1):45.
190. Kuwabata, S., and C.R. Martin. 1994. Mechanism of the amperometric response of a proposed glucose sensor based on a polypyrrole-tubule-impregnated membrane. Anal Chem 66 (17):2757.
191. Small, C.J., C.O. Too, and G.G. Wallace. 1997. Responsive conducting polymer-hydrogel composites. Polym Gels Networks 5:251.
192. Asberg, P., and O. Inganas. 2003. Hydrogels of a conducting conjugated polymer as 3-D enzyme electrode. Biosens Bioelectron 19:199.
193. Pich, A., Y. Lu, H.-J.P. Adler, T. Schmidt, and K.-F. Arndt. 2002. Dispersion polymerization of pyrrole in the presence of poly(vinyl methyl ether) microgels. Polymer 43:5723.
194. Douglass, P.M., S. Daunert, J.D. Patel, L.G. Bachas, K.-Q. He, and M.J. Madou. 2000. Biologically inspired, intelligent muscle material for sensing and responsive delivery of countermeasures. Soc Automot Eng 1.
195. Brahim, S., D. Narinesingh, and A. Guiseppi-Elie. 2002. Polypyrrole-hydrogel composites for the construction of clinically important biosensors. Biosens Bioelectron 17:53.
196. Rubio Retama, J., E. Lopez Cabarcos, D. Mecerreyes, and B. L’opez-Ruiz. 2004. Design of an amperometric biosensor using polypyrrole-microgel composites containing glucose oxidase. Bio-sens Bioelectron 20:1111.
197. Nishizawa, M., and I. Uchida. 1999. Microelectrode-based characterization systems for advanced materials in battery and sensor applications. Electrochim Acta 44:3629.
198. Matsue, T., M. Nishizawa, T. Sawaguchi, and T. Uchida. 1991. An enzyme switch sensitive to NADH. J Chem Soc Chem Commun 15:1029.
199. Bartlett, P.N., and P.R. Birkin. 1994. A microelectrochemical enzyme transistor responsive to glucose. Anal Chem 66:1552.
200. Bartlett, P.N., J.H. Wang, and E.N.K. Wallance. 1996. A microelectrochemical switch responsive to NADH. Chem Commun 3:359.
201. Bartlett, P.N, and P.R. Birkin. 1993. Enzyme switch responsive to glucose. Anal Chem 65:1118.
202. Barton, A.C., S.D. Collyer, F. Davis, D.D. Gornall, K.A. Law, E.C.D. Lawrence, D.W. Mills, S. Myler, J.A. Pritchard, M. Thompson, and S.P.J. Higson. 2004. Sonochemically fabricated microelectrode arrays for biosensors offering widespread applicability: Part I. Biosens Bioelectron 20:328.
203. Lee, C.S., J. Joo, S. Han, and S.K. Koh. 2005. An approach to durable PVDF cantilevers with highly conducting PEDOT=PSS (DMSO) electrodes. Sens Actuators A 373.
204. Mattioli-Belmonte, M., G. Giavaresi, G. Biagini, L. Virgili, M. Giacomini, M. Fini, F. Giantomassi, D. Natali, P. Torricelli, and R. Giardino. 2003. Tailoring biomaterial compatibility: In vivo tissue response versus in vitro cell behavior. Int J Artif Organs 26:1077.
205. Smela, E. 2003. Conjugated polymer actuators for biomedical applications. Adv Mater 15 (6):481.
206. Schmidt, C.E., V.R. Shastri, J.P. Vacanti, and R. Langer. 1997. Stimulation of neurite outgrowth using an electrically conducting polymer. Proc Natl Acad Sci USA 94:8948.
207. Rivers, T.J., T.W. Hudson, and C.E. Schmidt. 2002. Synthesis of a novel, biodegradable, electrically conducting polymer for biomedical applications. Adv Funct Mater 12 (1):33.
208. Inoue, S., M. Yasuzawa, and S. Imai. 2002. Preparation of glucose sensor using organopolysilox-ane=polypyrrole complex film. Proc 34th Chem Sens Symp 18 (A):136.
209. Persaud, K., and G.H. Dodd. 1982. Analysis of discrimination mechanisms in the mammalian olfactory system using a model nose. Nature 299:352.
210. Hodgins, D. 1995. The development of an electronic ‘nose’ for industrial and environmental applications. Sens Actuators B Chem 27 (1–3):255.
211. Nake, A., B. Dubreuil, C. Raynaud, and T. Talou. 2005. Outdoor in situ monitoring of volatile emissions from wastewater treatment plants with two portable technologies of electronic noses. Sens Actuators B Chem 106 (1):26.
212. Magan, N., A. Pavlou, and I. Chrysanthakis. 2001. Milk-sense: A volatile sensing system recognizes spoilage bacteria and yeasts in milk. Sens Actuators B Chem 72 (1):28.
213. Bourgeois, W., and R.M. Stuetz. 2002. Use of a chemical sensor array for detecting pollutants in domestic wastewater. Water Res 36 (18):4505.
214. Brezmes, J., Ma. L.L. Fructuoso, E. Llobet, X. Vilanova, I. Recasens, J. Orts, G. Saiz, and X. Correig. 2005. Evaluation of an electronic nose to assess fruit ripeness. IEEE Sens J 5 (1):97.
215. Guadarrama, A., J.A. Fernandez, M. Iniguez, J. Souto, and J.A. de Saja. 2001. Discrimination of wine aroma using an array of conducting polymer sensors in conjunction with solid-phase micro-extraction (SPME) technique. Sens Actuators B Chem 77 (1–2):401.
216. Guadarrama, A., M.L. Rodrìguez-Mendez, C. Sanz, J.L. Rìos, and J.A. de Saja. 2001. Electronic nose based on conducting polymers for the quality control of the olive oil aroma: Discrimination of quality, variety of olive and geographic origin. Anal Chim Acta 432 (2):283.
217. Fend, R., C. Bessant, A.J. Williams, and A.C. Woodman. 2004. Monitoring haemodialysis using electronic nose and chemometrics. Biosens Bioelectron 19:1581.
218. Lonergan, M.C., E.J. Severin, B.J. Doleman, S.A. Beaber, R.H. Grubbs, and N.S. Lewis. 1996. Array-based vapor sensing using chemically sensitive, carbon black-polymer resistors. Chem Mater 8:2298.
219. Hanson, C.W. III, and E.R. Thaler. 2005. Electronic nose prediction of a clinical pneumonia score: Biosensors and microbes. Anesthesiology 102:63.
220. Ramanathan, K., M.A. Bangar, M. Yun, W. Chen, A. Mulchandani, and N.V. Myung. 2004. Individually addressable conducting polymer nanowires array. Nanoletters 4 (7):1237.
221. Gerard, M., A. Chaubey, and B.D. Malhotra. 2002. Application of conducting polymers to biosensors. Biosens Bioelectron 17:345.
222. Guiseppi-Elie, A., G.G. Wallace, and T. Matsue. 1998. Chemical and biological sensors based on electrically conducting polymers. In Handbook of Conducting Polymers, 2nd Edn, Eds. T. Skotheim, R. Elsenbaumer, and J.R. Reynolds, Chapter 34, pp. 963–991. New York: Marcel Dekker.