A revolutionary approach facilitating co-expression of authentic human epidermal growth factor and basic fibroblast growth factor in both cytoplasm and culture medium of Escherichia coli

Applied Microbiology and Biotechnology

Volumn 97, Issue 20, 2013, Pages 9071-9080

  Kwong, Keith W Y ^a   Wong, W K R ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

bFGF; EGF; Escherichia coli; Excretion; Extracellular expression; FGF 2; hbFGF; hEGF; Human basic fibroblast growth factor; Human epidermal growth factor; Intein; Sce VMA; Secretion

Indexed keywords

BASIC FIBROBLAST GROWTH FACTOR; BFGF; EGF; EXCRETION; EXTRACELLULAR; FGF-2; HBFGF; HEGF; HUMAN EPIDERMAL GROWTH FACTOR; INTEIN; SCE VMA; SECRETION;

CELL CULTURE; PHYSIOLOGY; PROTEINS;

ESCHERICHIA COLI;

BACTERIAL VECTOR; FIBROBLAST GROWTH FACTOR 2; GAMMA UROGASTRONE; INTEIN; OUTER MEMBRANE PROTEIN A; VANILMANDELIC ACID;

BACTERIUM; CULTURE; CYTOPLASM; EXCRETION; GROWTH RATE; PEPTIDE; PROTEIN; SECRETION;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIUM CULTURE; CELL FRACTIONATION; CELL LYSATE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOKINE PRODUCTION; CYTOPLASM; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; SUPERNATANT;

ESCHERICHIA COLI;

3T3 CELLS; ANIMALS; CELL PROLIFERATION; CULTURE MEDIA; CYTOPLASM; EPIDERMAL GROWTH FACTOR; ESCHERICHIA COLI; EXTRACELLULAR SPACE; FIBROBLAST GROWTH FACTOR 2; GENE EXPRESSION; HUMANS; MICE; PROTEIN ENGINEERING; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS;

EID: 84885388820     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5090-8     Document Type: Article

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