Enhancement of extracellular production of a Cellulomonas fimi exoglucanase in Escherichia coli by the reduction of promoter strength

Enzyme and Microbial Technology

Volumn 20, Issue 7, 1997, Pages 482-488

  Lam, Tin Long ^a   Wong, Raymond S C ^a   Wong, Wan Keung R ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

Cellulases; Cellulose degradation; E. coli excretion systems; Extracellular production of exoglucanase

Indexed keywords

CELL CULTURE; CELLS; CELLULOSE; COLIFORM BACTERIA; DEGRADATION; GENES; WASTE TREATMENT;

CELLULOMONAS FIMI EXOGLUCANASE; ESCHERICHIA COLI; EXCRETION SYSTEM; PLASMID CURING;

ENZYMES;

CELLULASE; CELLULOSE; GLUCAN GLUCOSIDASE;

ARTICLE; CELL DEATH; CELL VIABILITY; CELLULOMONAS; COST EFFECTIVENESS ANALYSIS; CULTURE MEDIUM; ESCHERICHIA COLI; MOLECULAR STABILITY; NONHUMAN; PLASMID; PROMOTER REGION;

BETA-GLUCOSIDASE; DNA PRIMERS; ENDO-1,4-BETA XYLANASES; ESCHERICHIA COLI; GENE EXPRESSION; ISOPROPYL THIOGALACTOSIDE; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; XYLOSIDASES;

CELLULOMONAS; CELLULOMONAS FIMI; ESCHERICHIA COLI;

EID: 0031148990     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(96)00203-7     Document Type: Article

References (40)

1. Eveieigh, D. E. The fermentation of biomass. In: Biomass Utilization (Cote, W. A., Ed.). Plenum Press, New York, 1983, 365-391
2. Ryu, D. D. Y. and Mandels, M. Cellulases: Biosynthesis and applications. Enzyme Microb. Technol. 1980, 2, 91-101
3. Soltes, E. J. Cellulose: Elusive component of the plant cell wall. In: Biomass Utilization (Cote, W. A., Ed.). Plenum Press, New York, 1983, 271-298
4. Katzen, R. and Fowler, D. E. Ethanol from lignocellulosic wastes with utilization of recombinant bacteria. Appl. Biochem. Biotechnol. 1994, 45-46, 697-707
5. Holtzapple, M. T., Ripley, E. P., and Nikolaou, M. Saccharidification, fermentation, and protein recovery from low-temperature AFEX-treated coastal bermudagrass. Biotechnol. Bioeng. 1994, 44, 1122-1131
6. Philippidis, G. P., Smith, T. K., and Wyman, C. E. Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process. Biotechnol. Bioeng. 1993, 41, 846-853
7. Mandels, M. Cellulases. Ann. Rept. Ferm. Proc. 1982, 5, 35-78
8. Mullings, R. Measurement of saccharification by cellulases. Enzyme Microb. Technol. 1985, 7, 586-591
9. Béguin, P. and Aubert, J. P. The biological degradation of cellulose. FEMS Microbiol. Rev. 1994, 13, 25-58
10. Reese, E. T. and Mandels, M. Rolling with the times: Production and applications of Trichoderma reesei cellulase. Ann. Rept. Ferm. Proc. 1984, 7, 1-20
11. Whittle, D. J., Kilburn, D. G., Warren, R. A. J., Miller Jr., R. C. Molecular cloning of a Cellulomonas fimi cellulase gene in Escherichia coli. Gene 1982, 17, 139-145
12. Shoemaker, S., Schweickart, V., Ladner, M., Gelfand, D., Kwok, S., Myambo, K., and Innis, M. Molecular cloning of exocellobiohydrolase I derived from Trichoderma reesei. Bio/ Technology 1983, 1, 691-696
13. Gilkes, N. R., Kilburn, D. G., Langsford, M. L., Miller Jr., R. C., Wakarchuk, W. W., Warren, R. A. J., Whittle, D. J., and Wong, W. K. R. Isolation and characterization of Escherichia coli clones expressing cellulase genes from Cellulomonas fimi. J. Gen. Microbiol. 1984, 130, 1377-1384
14. Millet, J., Petre, D., Béguin, P., Raynaud, O., and Aubert, J. P. Cloning of ten distinct DNA fragments of Clostridium thermocellum coding for cellulases. FEMS Microbiol. Lett. 1985, 29, 145-149
15. Wong, W. K. R., Gerhard, B., Guo, Z. M., Kilburn, D. G., Warren, R. A. J., and Miller Jr., R. C. Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi. Gene 1986, 44, 315-325
16. Skipper, N., Sutherland, M., Davies, R. W., Kilburn, D., Miller Jr., R. C., Warren, A., and Wong, R. Secretion of a bacterial cellulase by yeast. Science 1985, 230, 958-960
17. Johnson, J. A., Wong, W. K. R., and Beatty, J. T. Expression of cellulase genes in Rhodobacter capsulatus by use of plasmid expression vectors. J. Bacteriol. 1986, 167, 604-610
18. Gilkes, N. R., Kilburn, D. G., Miller Jr., R. C., and Warren, R. A. J. A mutant of Escherichia coli that leaks cellulase activity encoded by cloned cellulase genes from Cellulomonas fimi. Bio/Technology 1984, 2, 259-263
19. Curry, C., Gilkes, N., O'Neill, G., Miller Jr., R. C., and Skipper, N. Expression and secretion of a Cellulomonas fimi exoglucanase in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 1988, 54, 476-484
20. Wong, W. K. R., Curry, C., Parekh, R. S., Parekh, S. R., Wayman, M., Davies, R. W., Kilburn, D. G., and Skipper, N. Wood hydrolysis by Cellulomonas fimi endoglucanase and exoglucanase coexpressed as secreted enzymes in Saccharomyces cerevisiae. Bio/Technology 1988, 6, 713-718
21. Kobayashi, T., Kato, C., Kudo, T., and Horikoshi, K. Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane is caused by insertional activation of the kil gene in plasmid pMB9. J. Bacteriol. 1986, 166, 728-732
22. Hsiung, H. M., Cantrell, A., Luirink, J., Oudega, B., Veros, A. J., and Becker, G. W. Use of bacteriocin release protein in E. coli for excretion of human growth hormone into the culture medium. Bio/ Technology 1989, 7, 267-271
23. Wong, W. K. R. and Sutherland, M. L. Excretion of heterologous proteins from E. coli. U.S. patent 5,223,407; 1993
24. O'Neill, G., Goh, S. H., Warren, R. A. J., Kilburn, D. G., and Miller Jr., R. C. Structure of the gene encoding the exoglucanase of Cellulomonas fimi. Gene 1986, 44, 325-330
25. deBoer, H. A., Comstock, L. J., and Vasser, M. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc. Natl. Acad. Sci. U.S.A. 1983, 80, 21-25
26. Russell, D. R. and Bennett, G. N. Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing. Gene 1982, 20, 231-243
27. Yanisch, P. C., Vieira, J., and Messing, J. Improved M13 phage cloning vectors and host strains: Nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 1985, 33, 103-119
28. Kunkel, T. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. U.S.A. 1985, 82, 488-492
29. Sambrook, J., Fritsch, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual 2nd Ed. Cold Spring Harbor Laboratory Press, New York, 1989
30. Deng, W. P. and Nickoloff, J. A. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 1992, 200, 81-88
31. Sanger, F., Nicklen, S., and Coulsen, A. R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 1977, 74, 5463-5467
32. Hawley, D. K. and McClure, W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucl. Acids Res. 1983, 11, 2237-2255
33. Gilkes, N. R., Langsford, M. L., Kilburn, D. G., Miller Jr., R. C., and Warren, R. A. J. Mode of action and substrate specificities of cellulases from cloned bacterial genes. J. Biol. Chem. 1984, 250, 10455-10459
34. Bradford, M. M. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254
35. O'Neill, G. P., Warren, R. A. J., Kilburn, D. G., and Miller Jr., R. C. Secretion of Cellulomonas fimi exoglucanase by Escherichia coli. Gene 1986, 44, 331-336
36. Warren, R. A. J., Beck, C. F., Gilkes, N. R., Kilburn, D. G., Langsford, M. L., Miller Jr., R. C., O'Neill, G. P., Scheufens, M., and Wong, W. K. R. Sequence conservation and region shuffling in an endoglucanase and an exoglucanase from Cellulomonas fimi. Proteins 1986, 1, 335-341
37. Takagi, H., Morinaga, Y., Tsuchiya, M., Ikemura, H., and Inouye, M. Control of folding of proteins secreted by a high expression secretion vector, pIN-III-ompA: 16-fold increase in production of active subtilisin E in Escherichia coli. Bio/Technology 1988, 6, 948-950
38. O'Neill, G. P., Kilburn, D. G., Warren, A. J., and Miller Jr., R. C. Overproduction from a cellulase gene with a high guanosine-plus-cytosine content in Escherichia coli. Appl. Environ. Microbiol. 1986, 52, 737-743
39. Guo, Z. M., Arfman, A., Ong, E., Gilkes, N. R., Kilburn, D. G., Warren, R. A. J., Miller Jr., R. C. Leakage of Cellulomonas fimi cellulases from Escherichia coli. FEMS Microbiol. Lett. 1988, 49, 279-283
40. Silverstone, A. E., Arditti, R. R., and Magasanik, B. Catabolite-insensitive revenants of lac promoter mutants. Proc. Natl. Acad. Sci. U.S.A. 1970, 66, 773-779