High-level expression, purification, and characterization of recombinant human basic fibroblast growth factor in Pichia pastoris

Protein Expression and Purification

Volumn 59, Issue 2, 2008, Pages 282-288

  Mu, Xupeng ^a   Kong, Ning ^a   Chen, Weili ^a   Zhang, Ting ^a   Shen, Mohan ^a   Yan, Weiqun ^a  

^a JILIN UNIVERSITY   (China)

Author keywords

Basic fibroblast growth factor; Pichia pastoris; Protein purification; Secretory expression

Indexed keywords

FIBROBLAST GROWTH FACTOR 2; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL STRAIN 3T3; CHEMISTRY; CULTURE MEDIUM; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR CLONING; MOUSE; PH; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FIBROBLAST GROWTH FACTOR 2; HUMANS; HYDROGEN-ION CONCENTRATION; MICE; NIH 3T3 CELLS; PICHIA; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 42749085054     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.02.009     Document Type: Article

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