A method for the amidation of recombinant peptides expressed as intein fusion proteins in Escherichia coli

Nature Biotechnology

Volumn 19, Issue 10, 2001, Pages 974-977

  Cottingham, Ian R ^a   Millar, Alan ^a   Emslie, Elisabeth ^a   Colman, Alan ^a   Schnieke, Angelika E ^a   McKee, Colin ^a  

^a PPL Therapeutics   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; DRUG PRODUCTS; ENZYMES; ESCHERICHIA COLI; PROTEINS;

COST EFFECTIVE PRODUCTION;

BIOTECHNOLOGY;

ADENOSINE TRIPHOSPHATASE; CALCITONIN; DISULFIDE; GLYCINE; GONADORELIN; HYBRID PROTEIN; INTEIN; PARATHYROID HORMONE; PEPTIDE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN;

AMIDATION; AMINO TERMINAL SEQUENCE; ARTICLE; CELL VACUOLE; DRUG MANUFACTURE; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; MILK; MOLECULAR SIZE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEIN SECRETION; TRANSGENE;

AMIDES; AMINO ACID SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTON-TRANSLOCATING ATPASES; RECOMBINANT FUSION PROTEINS; VACUOLAR PROTON-TRANSLOCATING ATPASES;

EID: 0034794891     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt1001-974     Document Type: Article

References (8)

1. Therapeutic peptides revisited
2. C-terminal amidated peptides: Production by the in vitro enzymatic amidation of glycine-extended peptides and the importance of the amide to bioactivity
3. Production of recombinant salmon calcitonin by in vitro amidation of an Escherichia coli produced precursor peptide
4. Production of biologically active salmon calcitonin in the milk of transgenic rabbits
5. Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein
6. A genetic system yields self-cleaving inteins for bioseparations
7. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid
8. Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi Gyrase A intein