Characterization of the first K+ channel blockers from the venom of the moroccan scorpion buthus occitanus paris

Toxicon

Volumn 75, Issue , 2013, Pages 168-176

  Martin Eauclaire, Marie France ^a   Céard, Brigitte ^a   Belghazi, Maya ^a   Lebrun, Régine ^b   Bougis, Pierre E ^a  

^a University of Mediterranée   (France)

^b AIX MARSEILLE UNIVERSITY   (France)

Author keywords

ELISA; HPLC; MALDI TOF MS; Potassium channels; Scorpion toxins

Indexed keywords

IODINE 125; KALIOTOXIN; KALIOTOXIN ANTIBODY; MARTENTOXIN; POTASSIUM CHANNEL KV1.3; SCORPION VENOM; TOXIN ANTIBODY; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; BINDING AFFINITY; BINDING COMPETITION; BINDING SITE; BRAIN SYNAPTOSOME; BUTHUS MARTENZI; BUTHUS OCCITANUS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE MUTATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IC 50; MALE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FAMILY; PROTEIN PURIFICATION; SCORPION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; TOXICITY TESTING; TOXIN ANALYSIS;

BUTHUS; BUTHUS OCCITANUS MARDOCHEI; BUTHUS OCCITANUS PARIS; MESOBUTHUS MARTENSII; MUS; RATTUS; SCORPIONES;

ELISA; HPLC; MALDI-TOF/MS; POTASSIUM CHANNELS; SCORPION TOXINS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; BRAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; INHIBITORY CONCENTRATION 50; MALE; MICE; MOLECULAR SEQUENCE DATA; PEPTIDES; POTASSIUM CHANNEL BLOCKERS; PROTEIN CONFORMATION; RADIOIMMUNOASSAY; RATS; SCORPION VENOMS; SCORPIONS; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SYNAPTOSOMES;

EID: 84885297474     PISSN: 00410101     EISSN: 18793150     Source Type: Journal    
DOI: 10.1016/j.toxicon.2013.03.004     Document Type: Article

References (30)

1. Abbas N., Belghazi M., Abdel-Mottaleb Y., Tytgat J., Bougis P.E., Martin-Eauclaire M.F. A new Kaliotoxin selective towards Kv1.3 and Kv1.2 but not Kv1.1 channels expressed in oocytes. Biochem. Biophys. Res. Commun. 2008, 376:525-530.
2. Ader C., Schneider R., Hornig S., Velisetty P., Wilson E.M., Lange A., Giller K., Ohmert I., Martin-Eauclaire M.F., Trauner D., Becker S., Pongs O., Baldus M. A structural link between inactivation and block of a K+ channel. Nat. Struct. Mol. Biol. 2008, 15:605-612.
3. Beeton C., Barbaria J., Giraud P., Devaux J., Benoliel A.M., Gola M., Sabatier J.M., Bernard D., Crest M., Béraud E. Selective blocking of voltage-gated K+ channels improves experimental autoimmune encephalomyelitis and inhibits T cell activation. J. Immunol. 2001, 166:936-944.
4. Bontems F., Roumestand C., Boyot P., Gilquin B., Doljansky Y., Menez A., Toma F. Three-dimensional structure of natural charybdotoxin in aqueous solution by 1H-NMR. Charybdotoxin possesses a structural motif found in other scorpion toxins. Eur. J. Biochem. 1991, 196:19-28.
5. Cahalan M.D., Chandy K.G. The functional network of ion channels in T lymphocytes. Immunol. Rev. 2009, 231:59-87.
6. Coetzee W.A., Amarillo Y., Chiu J., Chow A., Lau D., McCormack T., Moreno H., Nadal M.S., Ozaita A., Pountney D., Saganich M., Vega-Saenz de Miera E., Rudy B. Molecular diversity of K+ channels. Ann. N.Y. Acad. Sci. 1999, 868:233-285.
7. Crooks G.E., Hon G., Chandonia J.M., Brenner S.E. WebLogo: a sequence logo generator. Genome Res. 2004, 14:1188-1190.
8. Dauplais M., Lecoq A., Song J., Cotton J., Jamin N., Gilquin B., Roumestand C., Vita C., de Medeiros C.L., Rowan E.G., Harvey A.L., Ménez A. On the convergent evolution of animal toxins. Conservation of a diad of functional residues in potassium channel-blocking toxins with unrelated structures. J. Biol. Chem. 1997, 272:4302-4309.
9. Diego-García E., Peigneur S., Debaveye S., Gheldof E., Tytgat J., Caliskan F. Novel potassium channel blocker venom peptides from Mesobuthus gibbosus (Scorpiones: Buthidae). Toxicon 2012, 61:72-82.
10. Eswar N., Webb B., Marti-Renom M.A., Madhusudhan M.S., Eramian D., Shen M.Y., Pieper U., Sali A. Comparative protein structure modeling using Modeller. Curr. Protoc. Bioinformatics 2006, (Chapter 5), Unit 5.6.
11. Gao B., Peigneur S., Dalziel J., Tytgat J., Zhu S. Molecular divergence of two orthologous scorpion toxins affecting potassium channels. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2011, 159:313-321.
12. Granier C., Novotny J., Fontecilla-Camps J.C., Fourquet P., el Ayeb M., Bahraoui E. The antigenic structure of a scorpion toxin. Mol. Immunol. 1989, 26:503-513.
13. Gray E.G., Whittaker V.P. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J. Anat. 1962, 96:79-88.
14. Haley T.J., McCormick W.G. Pharmacological effects produced by intracerebral injection of drugs in the conscious mouse. Br. J. Pharmacol. Chemother. 1957, 12:12-15.
15. Honoré E., Guillemare E., Lesage F., Barhanin J., Lazdunski M. Injection of a K+ channel (Kv1.3) cRNA in fertilized eggs leads to functional expression in cultured myotomal muscle cells from Xenopus embryos. FEBS Lett. 1994, 348:259-262.
16. Jaravine V.A., Nolde D.E., Reibarkh M.J., Korolkova Y.V., Kozlov S.A., Pluzhnikov K.A., Grishin E.V., Arseniev A.S. Three-dimensional structure of toxin OSK1 from Orthochirus scrobiculosus scorpion venom. Biochemistry 1997, 36:1223-1232.
17. Ji Y.H., Wang W.X., Ye J.G., He L.L., Li Y.J., Yan Y.P., Zhou Z. Martentoxin, a novel K+-channel-blocking peptide: purification, cDNA and genomic cloning, and electrophysiological and pharmacological characterization. J. Neurochem. 2003, 84:325-335.
18. Lange A., Giller K., Hornig S., Martin-Eauclaire M.F., Pongs O., Becker S., Baldus M. Toxin-induced conformational changes in a potassium channel revealed by solid-state NMR. Nature 2006, 440:959-962.
19. Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., Lopez R., Thompson J.D., Gibson T.J., Higgins D.G. Clustal W and Clustal X version 2.0. Bioinformatics 2007, 23:2947-2948.
20. Long S.B., Campbell E.B., Mackinnon R. Crystal structure of a mammalian voltage-dependent Shaker family K+ channel. Science 2005, 309:897-903.
21. Martin-Eauclaire M.F., Bougis P.E. Potassium Channels Blockers from the Venom of Androctonus mauretanicus mauretanicus. J. Toxicol. 2012, 2012:103608.
22. Meki A., Mansuelle P., Laraba-Djebari F., Oughideni R., Rochat H., Martin-Eauclaire M.F. KTX3, the kaliotoxin from Buthus occitanus tunetanus scorpion venom: one of an extensive family of peptidyl ligands of potassium channels. Toxicon 2000, 38:105-111.
23. Mouhat S., Visan V., Ananthakrishnan S., Wulff H., Andreotti N., Grissmer S., Darbon H., De Waard M., Sabatier J.M. K+ channel types targeted by synthetic OSK1, a toxin from Orthochirus scrobiculosus scorpion venom. Biochem. J. 2005, 385:95-104.
24. Mourre C., Chernova M.N., Martin-Eauclaire M.F., Bessone R., Jacquet G., Gola M., Alper S.L., Crest M. Distribution in rat brain of binding sites of kaliotoxin, a blocker of Kv1.1 and Kv1.3 alpha-subunits. J. Pharmacol. Exp. Ther. 1999, 291:943-952.
25. Pettersen E.F., Goddard T.D., Huang C.C., Couch G.S., Greenblatt D.M., Meng E.C., Ferrin T.E. UCSF Chimera-a visualization system for exploratory research and analysis. J. Comput. Chem. 2004, 25:1605-1612.
26. Pieper U., Webb B.M., Barkan D.T., Schneidman-Duhovny D., Schlessinger A., Braberg H., Yang Z., Meng E.C., Pettersen E.F., Huang C.C., Datta R.S., Sampathkumar P., Madhusudhan M.S., Sjölander K., Ferrin T.E., Burley S.K., Sali A. ModBase, a database of annotated comparative protein structure models, and associated resources. Nucleic Acids Res. 2011, 39:D465-D474.
27. Ranganathan R., Lewis J.H., MacKinnon R. Spatial localization of the K+ channel selectivity filter by mutant cycle-based structure analysis. Neuron 1996, 16:131-139.
28. Romi R., Crest M., Gola M., Sampieri F., Jacquet G., Zerrouk H., Mansuelle P., Sorokine O., Van Dorsselaer A., Rochat H., Martin-Eauclaire M., Van Rietschoten J. Synthesis and characterization of kaliotoxin. Is the 26-32 sequence essential for potassium channel recognition?. J. Biol. Chem. 1993, 268:26302-26309.
29. Tytgat J., Chandy K.G., Garcia M.L., Gutman G.A., Martin-Eauclaire M.F., van der Walt J.J., Possani L.D. A unified nomenclature for short-chain peptides isolated from scorpion venoms: alpha-KTx molecular subfamilies. Trends Pharmacol. Sci. 1999, 20:444-447.
30. Wang Y.H., Cao Z.Y., He W.Y., Yan X.Z., Liu X., Liu H.Y., Liang X.T., Yu D.Q. 1H-NMR signal assignments and secondary structure analysis of martentoxin. J. Asian Nat. Prod. Res. 2006, 8:511-518.