Label-free high-throughput assays to screen and characterize novel lactate dehydrogenase inhibitors

Analytical Biochemistry

Volumn 441, Issue 2, 2013, Pages 115-122

  VanderPorten, Erica ^a   Frick, Lauren ^b   Turincio, Rebecca ^a   Thana, Peter ^a   LaMarr, William ^b   Liu, Yichin ^a  

^a GENENTECH INC   (United States)

^b Agilent Technologies   (United States)

Author keywords

Fluorescence kinetic assay; High throughput screen; Lactate dehydrogenase; Mass spectrometry

Indexed keywords

DRUG PRODUCTS; ENZYME ACTIVITY; KINETICS; MASS SPECTROMETERS; MASS SPECTROMETRY; MOLECULES; NUCLEOTIDES; REDOX REACTIONS;

FLUORESCENCE KINETICS; HIGH-THROUGHPUT ASSAYS; HIGH-THROUGHPUT SCREENS; INTRACELLULAR NICOTINAMIDE ADENINE DINUCLEOTIDE; LACTATE DEHYDROGENASE; MULTIPLE REACTION MONITORING; NICOTINAMIDE ADENINE DINUCLEOTIDES; SPECTROMETRIC METHODS;

FLUORESCENCE;

G PROTEIN COUPLED RECEPTOR; LACTATE DEHYDROGENASE; LACTATE DEHYDROGENASE INHIBITOR; LACTIC ACID; OXIDOREDUCTASE INHIBITOR; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UNCLASSIFIED DRUG;

ARTICLE; DOSE RESPONSE; ENZYME ACTIVITY; ENZYME MECHANISM; FOLLOW UP; HIGH THROUGHPUT SCREENING; IC 50; LIMIT OF DETECTION; LIMIT OF QUANTITATION; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL;

FLUORESCENCE KINETIC ASSAY; HIGH-THROUGHPUT SCREEN; LACTATE DEHYDROGENASE; MASS SPECTROMETRY;

ENZYME ASSAYS; ENZYME INHIBITORS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; L-LACTATE DEHYDROGENASE; LACTIC ACID; MASS SPECTROMETRY; NAD; NEOPLASMS; PYRUVIC ACID; SPECTROMETRY, FLUORESCENCE;

EID: 84884920570     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.07.003     Document Type: Article

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