Cloning, large scale over-expression in E. coli and purification of the components of the human LAT 1 (SLC7A5) amino acid transporter

Protein Journal

Volumn 32, Issue 6, 2013, Pages 442-448

  Galluccio, Michele ^a   Pingitore, Piero ^a   Scalise, Mariafrancesca ^a   Indiveri, Cesare ^a  

^a UNIVERSITY OF CALABRIA   (Italy)

Author keywords

Amino acid; CD98; E. coli; LAT1; Over expression; Transport

Indexed keywords

AMINO ACID TRANSPORTER; CD98 ANTIGEN; COMPLEMENTARY DNA; LAT PROTEIN; LAT1 PROTEIN; NICKEL; POLYPEPTIDE; PROTEIN; RECOMBINANT PROTEIN; THROMBIN; UNCLASSIFIED DRUG; UREA;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CELL LYSATE; CELL STRAIN HEK293; CHELATION; CONTROLLED STUDY; DNA VECTOR; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; LARGE SCALE PRODUCTION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PURIFICATION; SOLUBILIZATION; STRUCTURE ACTIVITY RELATION;

EID: 84884591979     PISSN: 15723887     EISSN: 18758355     Source Type: Journal    
DOI: 10.1007/s10930-013-9503-4     Document Type: Article

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