Over-expression in E. coli and purification of the human OCTN2 transport protein

Molecular Biotechnology

Volumn 50, Issue 1, 2012, Pages 1-7

  Galluccio, Michele ^a   Amelio, Linda ^a   Scalise, Mariafrancesca ^a   Pochini, Lorena ^a   Boles, Eckhard ^b   Indiveri, Cesare ^a  

^a UNIVERSITY OF CALABRIA   (Italy)

^b GOETHE UNIVERSITY   (Germany)

Author keywords

Codon bias; E. coli; OCTN2; Over expression; Purification

Indexed keywords

CHROMATOGRAPHY COLUMNS; CODON BIAS; E. COLI; ESCHERICHIA COLI ROSETTA; FUSION PROTEINS; GLUTATHIONE-S-TRANSFERASE; HUMAN SKIN FIBROBLAST; INCLUSION BODIES; OCTN2; OVER-EXPRESSION; PROTEIN YIELD; PURIFIED PROTEIN; ROSETTA; SDS-PAGE; TRANSPORT PROTEINS;

ANIMAL CELL CULTURE; CHELATION; CLONING; COLUMN CHROMATOGRAPHY; ESCHERICHIA COLI; GENE EXPRESSION; ORGANIC POLYMERS; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; UREA;

PROTEINS;

COMPLEMENTARY DNA; GLUTATHIONE TRANSFERASE; MEMBRANE PROTEIN; OCTN2 TRANSPORT PROTEIN; POLYPEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CODON; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; SKIN FIBROBLAST;

BIOTECHNOLOGY; CODON; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; HUMANS; ORGANIC CATION TRANSPORT PROTEINS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 84856294229     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-011-9406-6     Document Type: Article

References (19)

1. Wallin, E., & von Heijne, G. (1998). Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Science, 7, 1029-1038. (Pubitemid 28216544)
2. Palacin, M., Estevez, R., Bertran, J., & Zorzano, A. (1998). Molecular biology of mammalian plasma membrane amino acid transporters. Physiological Reviews, 78, 969-1054. (Pubitemid 28491947)
3. Lahjouji, K., Mitchell, G. A., & Qureshi, (2001). Carnitine transport by organic cation transporters and systemic carnitine deficiency. Molecular Genetics and Metabolism, 73, 287-297. (Pubitemid 32846367)
4. Daniel, H., & Kottra, G. (2004). The proton oligopeptide cotransporter family SLC15 in physiology and pharmacology. Pflugers Archiv, 447, 610-618. (Pubitemid 38241445)
5. Wright, E. M., & Turk, E. (2004). The sodium/glucose cotransport family SLC5. Pflugers Archiv, 447, 510-518. (Pubitemid 38241437)
6. Bröer, A., Cavanaugh, J. A., Rasko, J. E., & Bröer, S. (2006). The molecular basis of neutral aminoacidurias. Pflugers Archiv, 451, 511-517. (Pubitemid 41789791)
7. Palmieri, F. (2008). Diseases caused by defects of mitochondrial carriers: A review. Biochimica et Biophysica Acta, 1777, 564-578.
8. Tate, C. G. (2001). Overexpression of mammalian integral membrane proteins for structural studies. FEBS Letter, 504, 94-98. (Pubitemid 32787055)
9. Galluccio, M., Pochini, L., Amelio, L., Accardi, R., Tommasino, M., & Indiveri, C. (2009). Over-expression in E. coli and purification of the human OCTN1 transport protein. Protein Expression and Purification, 68, 215-220.
10. Tamai, I., Ohashi, R., Nezu, J. I., Sai, Y., Kobayashi, D., Oku, A., et al. (2000). Molecular and functional characterization of organic cation/carnitine transporter family in mice. The Journal of Biological Chemistry, 275, 40064-40072. (Pubitemid 32064631)
11. Lahjouji, K., Malo, C. K., Mitchell, G. A., & Qureshi, I. A. (2002). l-Carnitine transport in mouse renal and intestinal brush-border and basolateral membrane vesicles. Biochimica et Biophysica Acta, 1558, 82-93. (Pubitemid 34031957)
12. Tamai, I., Ohashi, R., Nezu, J., Yabuuchi, Y., Oku, A., Shimane, M., et al. (1998). Molecular and functional identification of sodium independent, high affinity human carnitine transporter OCTN2. The Journal of Biological Chemistry, 273, 20378-20382. (Pubitemid 28377603)
13. Pochini, L., Oppedisano, F., & Indiveri, C. (2004). Reconstitution into liposomes and functional characterization of the carnitine transporter from renal cell plasma membrane. Biochimica et Biophysica Acta, 1661, 78-86. (Pubitemid 38201573)
14. Pochini, L., Scalise, M., & Indiveri, C. (2009). Inactivation by omeprazole of the carnitine transporter (OCTN2) reconstituted in liposomes. Chemico-Biological Interactions, 179, 394-401.
15. Wu, X., Huang, W., Prasad, P. D., Seth, P., Rajan, D. P., Leibach, F. H., et al. (1999). Functional characteristics and tissue distribution pattern of organic cation transporter 2 (OCTN2), an organic cation/carnitine transporter. Journal of Pharmacology and Experimental Therapeutics, 290, 1482-1492. (Pubitemid 29400294)
16. Cohen, S. N., Chang, A. C., & Hsu, L. (1972). Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. The Proceedings of the National. Academy of Sciences of United States of America, 69, 2110-2114.
17. Dulley, J. R., & Grieve, P. A. (1975). A simple technique for eliminating interference by detergents in the Lowry method of protein determination. Analytical Biochemistry, 64, 136-141.
18. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
19. Niimura, Y., Terabe, M., Gojobori, T., & Miura, K. (2003). Comparative analysis of the base biases at the gene terminal portions in seven eukaryote genomes. Nucleic Acids Research, 31, 5195-5201. (Pubitemid 37441887)