Over-expression in Escherichia coli and characterization of two recombinant isoforms of human FAD synthetase

Biochemical and Biophysical Research Communications

Volumn 344, Issue 3, 2006, Pages 1008-1016

  Brizio, Carmen ^a   Galluccio, Michele ^b   Wait, Robin ^c   Torchetti, Enza Maria ^a   Bafunno, Valeria ^a   Accardi, Rosita ^d   Gianazza, Elisabetta ^e   Indiveri, Cesare ^b   Barile, Maria ^a,f  

^a UNIVERSITY OF BARI   (Italy)

^b UNIVERSITY OF CALABRIA   (Italy)

^c UNIVERSITY OF OXFORD   (United Kingdom)

^d INTERNATIONAL AGENCY FOR RESEARCH ON CANCER   (France)

^e UNIVERSITY OF MILAN   (Italy)

^f CNR   (Italy)

Author keywords

FAD synthesis; FAD synthetase; FADS; FLAD1; FMN adenylyl transferase; Isoforms

Indexed keywords

AMINO ACID; FLAVIN MONONUCLEOTIDE ADENYLYLTRANSFERASE; FLAVOPROTEIN; HYBRID PROTEIN; ISOENZYME; MAGNESIUM CHLORIDE; RECOMBINANT ENZYME; SYNTHETASE; UNCLASSIFIED DRUG; FMN ADENYLYLTRANSFERASE; NUCLEOTIDYLTRANSFERASE; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CELL FRACTIONATION; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ESCHERICHIA COLI; HUMAN; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SPECTROPHOTOMETRY; BIOSYNTHESIS; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; MOLECULAR GENETICS; PHYSIOLOGY; SEQUENCE HOMOLOGY;

ESCHERICHIA COLI;

AMINO ACID SEQUENCE; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; ISOENZYMES; MOLECULAR SEQUENCE DATA; NUCLEOTIDYLTRANSFERASES; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 33745244792     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.04.003     Document Type: Article

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