The Melanoma Antigens MELOE-1 and MELOE-2 Are Translated from a Bona Fide Polycistronic mRNA Containing Functional IRES Sequences

PLoS ONE

Volumn 8, Issue 9, 2013, Pages

  Carbonnelle, Delphine ^a,b,c   Vignard, Virginie ^a,b,c,d   Sehedic, Delphine ^a,b,c   Moreau Aubry, Agnes ^a,b,c   Florenceau, Laetitia ^a,b,c   Charpentier, Maud ^a,b,c   Mikulits, Wolfgang ^e   Labarriere, Nathalie ^a,b,c   Lang, François ^a,b,c  

^a INSERM   (France)

^b UNIVERSITÉ DE NANTES   (France)

^c CNRS   (France)

^d NANTES UNIVERSITY HOSPITAL   (France)

^e MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; MELANOMA ANTIGEN; MESSENGER RNA; POLYPEPTIDE; PROTEIN MELOE 1; PROTEIN MELOE 2; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ANTIGEN EXPRESSION; ANTIGEN PRESENTATION; ARTICLE; GENE CONSTRUCT; GENETIC CODE; HUMAN; HUMAN CELL; INTERNAL RIBOSOME ENTRY SITE; MELANOMA CELL; OPEN READING FRAME; RNA DEGRADATION;

ANTIGENS, NEOPLASM; GREEN FLUORESCENT PROTEINS; HUMANS; MELANOMA; NEOPLASM PROTEINS; PROTEIN BIOSYNTHESIS; RNA, MESSENGER; RNA, SMALL INTERFERING;

EID: 84884572707     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0075233     Document Type: Article

References (38)

1. Restifo NP, Dudley ME, Rosenberg SA, (2012) Adoptive immunotherapy for cancer: harnessing the T cell response. Nature reviews Immunology 12: 269-281.
2. van der Bruggen P, Stroobant V, Vigneron N, Van den Eynde B (2013) Peptide database: T cell-defined tumor antigens. Cancer Immun available from: http://www.cancerimmunity.org/peptide/.
3. Starck SR, Shastri N, (2011) Non-conventional sources of peptides presented by MHC class I. Cellular and molecular life sciences: CMLS. 68: 1471-1479.
4. Wang RF, Parkhurst MR, Kawakami Y, Robbins PF, Rosenberg SA, (1996) Utilization of an alternative open reading frame of a normal gene in generating a novel human cancer antigen. The Journal of experimental medicine 183: 1131-1140.
5. Wang RF, Johnston SL, Zeng G, Topalian SL, Schwartzentruber DJ, et al. (1998) A breast and melanoma-shared tumor antigen: T cell responses to antigenic peptides translated from different open reading frames. Journal of immunology 161: 3598-3606.
6. Aarnoudse CA, van den Doel PB, Heemskerk B, Schrier PI, (1999) Interleukin-2-induced, melanoma-specific T cells recognize CAMEL, an unexpected translation product of LAGE-1. International journal of cancer Journal international du cancer 82: 442-448.
7. Rosenberg SA, Tong-On P, Li Y, Riley JP, El-Gamil M, et al. (2002) Identification of BING-4 cancer antigen translated from an alternative open reading frame of a gene in the extended MHC class II region using lymphocytes from a patient with a durable complete regression following immunotherapy. Journal of immunology 168: 2402-2407.
8. Moreau-Aubry A, Le Guiner S, Labarriere N, Gesnel MC, Jotereau F, et al. (2000) A processed pseudogene codes for a new antigen recognized by a CD8(+) T cell clone on melanoma. The Journal of experimental medicine 191: 1617-1624.
9. Xiong Z, Liu E, Yan Y, Silver RT, Yang F, et al. (2006) An unconventional antigen translated by a novel internal ribosome entry site elicits antitumor humoral immune reactions. J Immunol 177: 4907-4916.
10. Godet Y, Moreau-Aubry A, Guilloux Y, Vignard V, Khammari A, et al. (2008) MELOE-1 is a new antigen overexpressed in melanomas and involved in adoptive T cell transfer efficiency. J Exp Med 205: 2673-2682.
11. Godet Y, Moreau-Aubry A, Mompelat D, Vignard V, Khammari A, et al. (2010) An additional ORF on meloe cDNA encodes a new melanoma antigen, MELOE-2, recognized by melanoma-specific T cells in the HLA-A2 context. Cancer Immunol Immunother 59: 431-439.
12. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet 36: 40-45.
13. Carninci P, Kasukawa T, Katayama S, Gough J, Frith MC, et al. (2005) The transcriptional landscape of the mammalian genome. Science 309: 1559-1563.
14. Savard J, Marques-Souza H, Aranda M, Tautz D, (2006) A segmentation gene in tribolium produces a polycistronic mRNA that codes for multiple conserved peptides. Cell 126: 559-569.
15. Galindo MI, Pueyo JI, Fouix S, Bishop SA, Couso JP, (2007) Peptides encoded by short ORFs control development and define a new eukaryotic gene family. PLoS Biol 5: e106.
16. Kondo T, Hashimoto Y, Kato K, Inagaki S, Hayashi S, et al. (2007) Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA. Nat Cell Biol 9: 660-665.
17. Tautz D, (2009) Polycistronic peptide coding genes in eukaryotes-how widespread are they? Briefings in functional genomics & proteomics 8: 68-74.
18. Kondo T, Plaza S, Zanet J, Benrabah E, Valenti P, et al. (2010) Small peptides switch the transcriptional activity of Shavenbaby during Drosophila embryogenesis. Science 329: 336-339.
19. Godet Y, Desfrancois J, Vignard V, Schadendorf D, Khammari A, et al. (2010) Frequent occurrence of high affinity T cells against MELOE-1 makes this antigen an attractive target for melanoma immunotherapy. Eur J Immunol 40: 1786-1794.
20. Butterfield LH, Stoll TC, Lau R, Economou JS, (1997) Cloning and analysis of MART-1/Melan-A human melanoma antigen promoter regions. Gene 191: 129-134.
21. Petz M, Kozina D, Huber H, Siwiec T, Seipelt J, et al. (2007) The leader region of Laminin B1 mRNA confers cap-independent translation. Nucleic Acids Res 35: 2473-2482.
22. Jang SK, Krausslich HG, Nicklin MJ, Duke GM, Palmenberg AC, et al. (1988) A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Journal of virology 62: 2636-2643.
23. Holcik M, Graber T, Lewis SM, Lefebvre CA, Lacasse E, et al. (2005) Spurious splicing within the XIAP 5′ UTR occurs in the Rluc/Fluc but not the betagal/CAT bicistronic reporter system. RNA 11: 1605-1609.
24. Kozak M, (2007) Lessons (not) learned from mistakes about translation. Gene 403: 194-203.
25. Andrews J, Smith M, Merakovsky J, Coulson M, Hannan F, et al. (1996) The stoned locus of Drosophila melanogaster produces a dicistronic transcript and encodes two distinct polypeptides. Genetics 143: 1699-1711.
26. Brogna S, Ashburner M, (1997) The Adh-related gene of Drosophila melanogaster is expressed as a functional dicistronic messenger RNA: multigenic transcription in higher organisms. The EMBO journal 16: 2023-2031.
27. Kanamori Y, Hayakawa Y, Matsumoto H, Yasukochi Y, Shimura S, et al. (2010) A eukaryotic (insect) tricistronic mRNA encodes three proteins selected by context-dependent scanning. J Biol Chem 285: 36933-36944.
28. Liu H, Jang JK, Graham J, Nycz K, McKim KS, (2000) Two genes required for meiotic recombination in Drosophila are expressed from a dicistronic message. Genetics 154: 1735-1746.
29. Gray TA, Saitoh S, Nicholls RD, (1999) An imprinted, mammalian bicistronic transcript encodes two independent proteins. Proceedings of the National Academy of Sciences of the United States of America 96: 5616-5621.
30. Lee SJ, (1991) Expression of growth/differentiation factor 1 in the nervous system: conservation of a bicistronic structure. Proceedings of the National Academy of Sciences of the United States of America 88: 4250-4254.
31. Reiss J, Dorche C, Stallmeyer B, Mendel RR, Cohen N, et al. (1999) Human molybdopterin synthase gene: genomic structure and mutations in molybdenum cofactor deficiency type B. American journal of human genetics. 64: 706-711.
32. Bahar Halpern K, Veprik A, Rubins N, Naaman O, Walker MD, (2012) GPR41 gene expression is mediated by internal ribosome entry site (IRES)-dependent translation of bicistronic mRNA encoding GPR40 and GPR41 proteins. The Journal of biological chemistry 287: 20154-20163.
33. Wall AA, Phillips AM, Kelly LE, (2005) Effective translation of the second cistron in two Drosophila dicistronic transcripts is determined by the absence of in-frame AUG codons in the first cistron. The Journal of biological chemistry 280: 27670-27678.
34. Ramanathan P, Guo J, Whitehead RN, Brogna S, (2008) The intergenic spacer of the Drosophila Adh-Adhr dicistronic mRNA stimulates internal translation initiation. RNA biology 5: 149-156.
35. Spriggs KA, Bushell M, Mitchell SA, Willis AE, (2005) Internal ribosome entry segment-mediated translation during apoptosis: the role of IRES-trans-acting factors. Cell death and differentiation 12: 585-591.
36. Silvera D, Formenti SC, Schneider RJ, (2010) Translational control in cancer. Nature reviews Cancer 10: 254-266.
37. Petz M, Them N, Huber H, Beug H, Mikulits W, (2012) La enhances IRES-mediated translation of laminin B1 during malignant epithelial to mesenchymal transition. Nucleic Acids Res 40: 290-302.
38. Jean D, Rousselet N, Frade R, (2008) Cathepsin L expression is up-regulated by hypoxia in human melanoma cells: role of its 5′-untranslated region. The Biochemical journal 413: 125-134.