Anti-HIV Activity of Human Defensin 5 in Primary CD4+ T Cells under Serum-Deprived Conditions Is a Consequence of Defensin-Mediated Cytotoxicity

PLoS ONE

Volumn 8, Issue 9, 2013, Pages

  Ding, Jian ^a   Tasker, Carley ^b,c   Valere, Kimyata ^b,c   Sihvonen, Tiina ^a   Descalzi Montoya, Dante B ^b,c   Lu, Wuyuan ^d   Chang, Theresa L ^a,b  

^a PUBLIC HEALTH RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY   (United States)

^c The State University of New Jersey   (United States)

^d UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE RECEPTOR CCR5; DEFENSIN;

APOPTOSIS; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL DEATH; CELL ISOLATION; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; CYTOTOXICITY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; NEISSERIA GONORRHOEAE; VIRUS INFECTIVITY; VIRUS REPLICATION; VIRUS TRANSMISSION;

ALPHA-DEFENSINS; APOPTOSIS; CD4-POSITIVE T-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CULTURE MEDIA, SERUM-FREE; FLOW CYTOMETRY; HIV INFECTIONS; HIV-1; HUMANS;

EID: 84884564230     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0076038     Document Type: Article

References (48)

1. Ganz T, (2003) Defensins: antimicrobial peptides of innate immunity. Nat Rev Immunol 3: 710-720.
2. Salzman NH, (2010) Paneth cell defensins and the regulation of the microbiome: detente at mucosal surfaces. Gut microbes 1: 401-406.
3. Lehrer RI, Lu W, (2012) alpha-Defensins in human innate immunity. Immunological reviews 245: 84-112.
4. Daher KA, Selsted ME, Lehrer RI, (1986) Direct inactivation of viruses by human granulocyte defensins. J Virol 60: 1068-1074.
5. Ding J, Chou YY, Chang TL, (2009) Defensins in viral infections. Journal of innate immunity 1: 413-420.
6. Meyerholz DK, Grubor B, Gallup JM, Lehmkuhl HD, Anderson RD, et al. (2004) Adenovirus-mediated gene therapy enhances parainfluenza virus 3 infection in neonatal lambs. J Clin Microbiol 42: 4780-4787.
7. Ryan LK, Dai J, Yin Z, Megjugorac N, Uhlhorn V, et al. (2011) Modulation of human {beta}-defensin-1 (hBD-1) in plasmacytoid dendritic cells (PDC), monocytes, and epithelial cells by influenza virus, Herpes simplex virus, and Sendai virus and its possible role in innate immunity. Journal of leukocyte biology 90(2): 343-356.
8. Wehkamp J, Chu H, Shen B, Feathers RW, Kays RJ, et al. (2006) Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues. FEBS letters 580: 5344-5350.
9. Guadalupe M, Reay E, Sankaran S, Prindiville T, Flamm J, et al. (2003) Severe CD4+ T-cell depletion in gut lymphoid tissue during primary human immunodeficiency virus type 1 infection and substantial delay in restoration following highly active antiretroviral therapy. J Virol 77: 11708-11717.
10. Jones DE, Bevins CL, (1992) Paneth cells of the human small intestine express an antimicrobial peptide gene. J Biol Chem 267: 23216-23225.
11. Quayle AJ, Porter EM, Nussbaum AA, Wang YM, Brabec C, et al. (1998) Gene expression, immunolocalization, and secretion of human defensin-5 in human female reproductive tract. Am J Pathol 152: 1247-1258.
12. Porter E, Yang H, Yavagal S, Preza GC, Murillo O, et al. (2005) Distinct defensin profiles in Neisseria gonorrhoeae and Chlamydia trachomatis urethritis reveal novel epithelial cell-neutrophil interactions. Infect Immun 73: 4823-4833.
13. Fan SR, Liu XP, Liao QP, (2008) Human defensins and cytokines in vaginal lavage fluid of women with bacterial vaginosis. Int J Gynaecol Obstet 103(1): 50-54.
14. Klotman ME, Rapista A, Teleshova N, Micsenyi A, Jarvis GA, et al. (2008) Neisseria gonorrhoeae-Induced Human Defensins 5 and 6 Increase HIV Infectivity: Role in Enhanced Transmission. J Immunol 180: 6176-6185.
15. Zhu T, Mo H, Wang N, Nam DS, Cao Y, et al. (1993) Genotypic and phenotypic characterization of HIV-1 patients with primary infection. Science 261: 1179-1181.
16. van't Wout AB, Kootstra NA, Mulder-Kampinga GA, Albrecht-van Lent N, Scherpbier HJ, et al. (1994) Macrophage-tropic variants initiate human immunodeficiency virus type 1 infection after sexual, parenteral, and vertical transmission. The Journal of clinical investigation 94: 2060-2067.
17. Rapista A, Ding J, Benito B, Lo YT, Neiditch MB, et al. (2011) Human defensins 5 and 6 enhance HIV-1 infectivity through promoting HIV attachment. Retrovirology 8: 45.
18. Furci L, Tolazzi M, Sironi F, Vassena L, Lusso P, (2012) Inhibition of HIV-1 infection by human alpha-defensin-5, a natural antimicrobial peptide expressed in the genital and intestinal mucosae. PloS one 7: e45208.
19. Zegels G, Van Raemdonck GA, Tjalma WA, Van Ostade XW, (2010) Use of cervicovaginal fluid for the identification of biomarkers for pathologies of the female genital tract. Proteome science 8: 63.
20. Shaw JL, Smith CR, Diamandis EP, (2007) Proteomic analysis of human cervico-vaginal fluid. Journal of proteome research 6: 2859-2865.
21. Zegels G, Van Raemdonck GA, Coen EP, Tjalma WA, Van Ostade XW, (2009) Comprehensive proteomic analysis of human cervical-vaginal fluid using colposcopy samples. Proteome science 7: 17.
22. Venkataraman N, Cole AL, Svoboda P, Pohl J, Cole AM, (2005) Cationic polypeptides are required for anti-HIV-1 activity of human vaginal fluid. J Immunol 175: 7560-7567.
23. Ficarra M, Ibana JS, Poretta C, Ma L, Myers L, et al. (2008) A distinct cellular profile is seen in the human endocervix during Chlamydia trachomatis infection. Am J Reprod Immunol 60: 415-425.
24. Wu Z, Ericksen B, Tucker K, Lubkowski J, Lu W, (2004) Synthesis and characterization of human alpha-defensins 4-6. The journal of peptide research: official journal of the American Peptide Society 64: 118-125.
25. Brown BK, Darden JM, Tovanabutra S, Oblander T, Frost J, et al. (2005) Biologic and genetic characterization of a panel of 60 human immunodeficiency virus type 1 isolates, representing clades A, B, C, D, CRF01_AE, and CRF02_AG, for the development and assessment of candidate vaccines. Journal of virology 79: 6089-6101.
26. Chen BK, Saksela K, Andino R, Baltimore D, (1994) Distinct modes of human immunodeficiency virus type 1 proviral latency revealed by superinfection of nonproductively infected cell lines with recombinant luciferase-encoding viruses. J Virol 68: 654-660.
27. Connor RI, Sheridan KE, Ceradini D, Choe S, Landau NR, (1997) Change in coreceptor use coreceptor use correlates with disease progression in HIV-1-infected individuals. J Exp Med 185: 621-628.
28. Chang TL, Vargas J Jr, DelPortillo A, Klotman ME, (2005) Dual role of alpha-defensin-1 in anti-HIV-1 innate immunity. J Clin Invest 115: 765-773.
29. Martinez-Lorenzo MJ, Anel A, Gamen S, Monle NL, Lasierra P, et al. (1999) Activated human T cells release bioactive Fas ligand and APO2 ligand in microvesicles. Journal of immunology 163: 1274-1281.
30. O'Doherty U, Swiggard WJ, Malim MH, (2000) Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding. Journal of virology 74: 10074-10080.
31. Guo J, Wang W, Yu D, Wu Y, (2011) Spinoculation triggers dynamic actin and cofilin activity that facilitates HIV-1 infection of transformed and resting CD4 T cells. Journal of virology 85: 9824-9833.
32. Rapista A, Ding J, Benito B, Lo YT, Neiditch MB, et al. (2011) Human defensins 5 and 6 enhance HIV-1 infectivity through promoting HIV attachment. Retrovirology 8: 45.
33. Seidel A, Ye Y, de Armas LR, Soto M, Yarosh W, et al. (2010) Cyclic and acyclic defensins inhibit human immunodeficiency virus type-1 replication by different mechanisms. PloS one 5: e9737.
34. Lehrer RI, Jung G, Ruchala P, Andre S, Gabius HJ, et al. (2009) Multivalent binding of carbohydrates by the human {alpha}-defensin, HD5. J Immunol 183: 480-490.
35. Ding J, Chang TL, (2012) TLR2 activation enhances HIV nuclear import and infection through T cell activation-independent and-dependent pathways. Journal of immunology 188: 992-1001.
36. Aarbiou J, Tjabringa GS, Verhoosel RM, Ninaber DK, White SR, et al. (2006) Mechanisms of cell death induced by the neutrophil antimicrobial peptides alpha-defensins and LL-37. Inflammation research: official journal of the European Histamine Research Society [et al] 55: 119-127.
37. Porter EM, Liu L, Oren A, Anton PA, Ganz T, (1997) Localization of human intestinal defensin 5 in Paneth cell granules. Infect Immun 65: 2389-2395.
38. Yang D, Oppenheim JJ, (2004) Antimicrobial proteins act as "alarmins" in joint immune defense. Arthritis and rheumatism 50: 3401-3403.
39. Rehaume LM, Hancock RE, (2008) Neutrophil-derived defensins as modulators of innate immune function. Crit Rev Immunol 28: 185-200.
40. Hazrati E, Galen B, Lu W, Wang W, Ouyang Y, et al. (2006) Human alpha- and beta-defensins block multiple steps in herpes simplex virus infection. J Immunol 177: 8658-8666.
41. Dugan AS, Maginnis MS, Jordan JA, Gasparovic ML, Manley K, et al. (2008) Human alpha-defensins inhibit BK virus infection by aggregating virions and blocking binding to host cells. J Biol Chem 283: 31125-31132.
42. Smith JG, Nemerow GR, (2008) Mechanism of adenovirus neutralization by Human alpha-defensins. Cell Host Microbe 3: 11-19.
43. Buck CB, Day PM, Thompson CD, Lubkowski J, Lu W, et al. (2006) Human {alpha}-defensins block papillomavirus infection. Proc Natl Acad Sci U S A 103: 1516-1521.
44. Gounder AP, Wiens ME, Wilson SS, Lu W, Smith JG, (2012) Critical determinants of human alpha-defensin 5 activity against non-enveloped viruses. The Journal of biological chemistry 287: 24554-24562.
45. Ding J, Rapista A, Teleshova N, Lu W, Klotman ME, et al. (2011) Mucosal human defensins 5 and 6 antagonize the anti-HIV activity of candidate polyanion microbicides. Journal of innate immunity 3: 208-212.
46. Classen S, Zander T, Eggle D, Chemnitz JM, Brors B, et al. (2007) Human resting CD4+ T cells are constitutively inhibited by TGF beta under steady-state conditions. Journal of immunology 178: 6931-6940.
47. Li C, Capan E, Zhao Y, Zhao J, Stolz D, et al. (2006) Autophagy is induced in CD4+ T cells and important for the growth factor-withdrawal cell death. Journal of immunology 177: 5163-5168.
48. Novelli F, Di Pierro F, Francia di Celle P, Bertini S, Affaticati P, et al. (1994) Environmental signals influencing expression of the IFN-gamma receptor on human T cells control whether IFN-gamma promotes proliferation or apoptosis. Journal of immunology 152: 496-504.