A quantitative method to identify microRNAs targeting a messenger RNA using a 3′UTR RNA affinity technique

Analytical Biochemistry

Volumn 443, Issue 1, 2013, Pages 1-12

  Shi, Miao ^a   Han, Weiguo ^a   Spivack, Simon D ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Gene modulation; Gene targeting; miRNA; miRNA:mRNA binding; mRNA

Indexed keywords

BINDING ENERGY; CLONE CELLS; CLONING; GENES; POLYMERASE CHAIN REACTION; PROTEINS;

CLONING AND SEQUENCING; GENE TARGETING; MESSENGER RNAS (MRNA); MIRNA; MRNA; MRNA BINDING; QUANTITATIVE METHOD; QUANTITATIVE POLYMERASE CHAIN REACTION;

RNA;

BIOTIN; MESSENGER RNA; MICRORNA; MICRORNA 150; MICRORNA 200; MICRORNA 200C; SMAD4 PROTEIN; TRANSCRIPTION FACTOR ZEB1; UNCLASSIFIED DRUG;

3' UNTRANSLATED REGION; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; GENE CONTROL; GENE DOSAGE; GENE EXPRESSION; GENE SEQUENCE; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEIC ACID IMMOBILIZATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REPORTER GENE; REVERSE TRANSCRIPTION;

GENE MODULATION; GENE TARGETING; MIRNA; MIRNA:MRNA BINDING; MRNA;

3' UNTRANSLATED REGIONS; ANIMALS; BIOLOGICAL ASSAY; BIOTIN; CLONING, MOLECULAR; EPITHELIAL CELLS; GENE EXPRESSION REGULATION; GENES, REPORTER; HOMEODOMAIN PROTEINS; HUMANS; IMMOBILIZED NUCLEIC ACIDS; LIVER; LUCIFERASES; MICE; MICRORNAS; MICROSPHERES; REAL-TIME POLYMERASE CHAIN REACTION; RESPIRATORY MUCOSA; SENSITIVITY AND SPECIFICITY; SMAD4 PROTEIN; STREPTAVIDIN; TRANSCRIPTION FACTORS;

EID: 84884521280     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.08.002     Document Type: Article

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