Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function

Cryobiology

Volumn 67, Issue 2, 2013, Pages 193-200

  Germann, Anja ^a   Oh, Young Joo ^a   Schmidt, Tomm ^a   Schön, Uwe ^a   Zimmermann, Heiko ^a,b   von Briesen, Hagen ^a  

^a FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

Author keywords

Cryopreservation; ELISpot; Optimal sample storage; PBMC functionality; PBMC recovery

Indexed keywords

GAMMA INTERFERON; LIQUID NITROGEN;

ANTIGEN SPECIFICITY; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; ENZYME LINKED IMMUNOSPOT ASSAY; HUMAN; HUMAN CELL; IMMUNE RESPONSE; INTERFERON PRODUCTION; LOW TEMPERATURE; LYMPHOCYTE FUNCTION; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; ROOM TEMPERATURE; STORAGE TEMPERATURE; T LYMPHOCYTE; TEMPERATURE; THAWING;

CRYOPRESERVATION; ELISPOT; OPTIMAL SAMPLE STORAGE; PBMC FUNCTIONALITY; PBMC RECOVERY;

BLOOD PRESERVATION; CELL SURVIVAL; COLD TEMPERATURE; CRYOPRESERVATION; EQUIPMENT DESIGN; FREEZING; HUMANS; LEUKOCYTES, MONONUCLEAR; T-LYMPHOCYTES;

EID: 84884412580     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2013.06.012     Document Type: Article

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