Substantial improvements in performance indicators achieved in a peripheral blood mononuclear cell cryopreservation quality assurance program using single donor samples

Clinical and Vaccine Immunology

Volumn 14, Issue 1, 2007, Pages 52-59

  Dyer, Wayne B ^a,b   Pett, Sarah L ^c   Sullivan, John S ^a,b   Emery, Sean ^c   Cooper, David A ^c   Kelleher, Anthony D ^c,d   Lloyd, Andrew ^d,e   Lewin, Sharon R ^f,g  

^a AUSTRALIAN RED CROSS BLOOD SERVICE   (Australia)

^b University of Sydney   (Australia)

^c UNIVERSITY OF NEW SOUTH WALES   (Australia)

^d UNIVERSITY OF NEW SOUTH WALES   (Australia)

^e PRINCE OF WALES HOSPITAL   (Australia)

^f ALFRED HOSPITAL   (Australia)

^g MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ACCREDITATION; ARTICLE; AUSTRALIA; BLOOD DONOR; CELL FRACTIONATION; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENT; LABORATORY; LEUKOCYTE FUNCTION; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; QUALITY CONTROL; BLOOD SAMPLING; BLOOD STORAGE; HEALTH CARE QUALITY; INSTRUMENTATION; LABORATORY DIAGNOSIS; METHODOLOGY; MONONUCLEAR CELL; STANDARD;

BLOOD PRESERVATION; BLOOD SPECIMEN COLLECTION; CRYOPRESERVATION; HUMANS; LEUKOCYTES, MONONUCLEAR; QUALITY ASSURANCE, HEALTH CARE; SPECIMEN HANDLING;

EID: 34247869117     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00214-06     Document Type: Article

References (15)

1. Betensky, R. A., E. Connick, J. Devers, A. L. Landay, M. Nokta, S. Plaeger, H. Rosenblatt, J. L. Schmitz, F. Valentine, D. Wara, A. Weinberg, and H. M. Lederman. 2000. Shipment impairs lymphocyte proliferative responses to microbial antigens. Clin. Diagn. Lab. Immunol. 7:759-763.
2. Betts, M. R., J. P. Casazza, and R. A. Koup. 2001. Monitoring HIV-specific CD8+ T cell responses by intracellular cytokine production. Immunol. Lett. 79:117-125.
3. Currier, J. R., E. G. Kuta, E. Turk, L. B. Earhart, L. Loomis-Price, S. Janetzki, G. Ferrari, D. L. Birx, and J. H. Cox. 2002. A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assays. J. Immunol. Methods 260:157-172.
4. Disis, M. L., C. dela Rosa, V. Goodell, L. Y. Kuan, J. C. Chang, K. Kuus-Reichel, T. M. Clay, H. K. Lyerly, S. Bhatia, S. A. Ghanekar, V. C. Maino, and H. T. Maecker. 2006. Maximizing the retention of antigen specific lymphocyte function after cryopreservation. J. Immunol. Methods 308: 13-18.
5. Dyer, W. B., H. Kuipers, M. W. Coolen, A. F. Geczy, J. Forrester, C. Workman, and J. S. Sullivan. 2002. Correlates of antiviral immune restoration in acute and chronic HIV-1 infection: sustained viral suppression and normalisation of T cell subsets. AIDS Res. Hum. Retrovir. 18:999-1010.
6. Fowke, K. R., J. Behnke, C. Hanson, K. Shea, and L. M. Cosentino. 2000. Apoptosis: a method for evaluating the cryopreservation of whole blood and peripheral blood mononuclear cells. J. Immunol. Methods 244:139-144.
7. Kleeberger, C. A., R. H. Lyles, J. B. Margolick, C. R. Rinaldo, J. P. Phair, and J. V. Giorgi. 1999. Viability and recovery of peripheral blood mononuclear cells cryopreserved for up to 12 years in a multicenter study. Clin. Diagn. Lab. Immunol. 6:14-19.
8. Kreher, C. R., M. T. Dittrich, R. Guerkov, B. O. Boehm, and M. Tary-Lehmann. 2003. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods 278:79-93.
9. Matthews, J. N. S., D. G. Altman, M. J. Campbell, and P. Royston. 1990. Analysis of serial measurements in medical research. BMJ 300:230-235.
10. Pandolfi, F., M. Pierdominici, A. Oliva, G. D'Offizi, I. Mezzaroma, B. Mollicone, A. Giovannetti, L. Rainaldi, I. Quinti, and F. Aiuti. 1995. Apoptosis-related mortality in vitro of mononuclear cells from patients with HIV infection correlates with disease severity and progression. J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 9:450-458.
11. Roos, M. T., N. A. de Leeuw, F. A. Claessen, H. G. Huisman, N. A. Kootstra, L. Meyaard, P. T. Schellekens, H. Schuitemaker, and F. Miedema. 1994. Viro-immunological studies in acute HIV-1 infection. AIDS 8:1533-1538.
12. Smith, J. G., X. Liu, R. M. Kaufhold, J. Clair, and M. J. Caulfield. 2001. Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune responses to varicella-zoster virus. Clin. Diagn. Lab. Immunol. 8:871-879.
13. Tree, T. I., B. O. Roep, and M. Peakman. 2004. Enhancing the sensitivity of assays to detect T cell reactivity: the effect of cell separation and cryopreservation media. Ann. N. Y. Acad. Sci. 1037:26-32.
14. Weinberg, A., R. A. Betensky, L. Zhang, and G. Ray. 1998. Effect of shipment, storage, anticoagulant, and cell separation on lymphocyte proliferation assays for human immunodeficiency virus-infected patients. Clin. Diagn. Lab. Immunol. 5:804-807.
15. Weinberg, A., L. Zhang, D. Brown, A. Erice, B. Polsky, M. S. Hirsch, S. Owens, and K. Lamb. 2000. Viability and functional activity of cryopreserved mononuclear cells. Clin. Diagn. Lab. Immunol. 7:714-716.