A flow cytometric method for the analysis of macrophages in the vascular wall

Journal of Immunological Methods

Volumn 396, Issue 1-2, 2013, Pages 33-43

  Moore, Jeffrey P ^a   Sakkal, Samy ^b   Bullen, Michelle L ^a   Kemp Harper, Barbara K ^a   Ricardo, Sharon D ^a   Sobey, Christopher G ^a   Drummond, Grant R ^a  

^a MONASH UNIVERSITY   (Australia)

^b VICTORIA UNIVERSITY   (Australia)

Author keywords

Aorta; Autofluorescence; Flow cytometry; Hypercholesterolemi; Hypertension; Macrophage

Indexed keywords

ANGIOTENSIN II; ANTIBODY CONJUGATE; ANTIGEN; APOLIPOPROTEIN E; B7 ANTIGEN; CD45 ANTIGEN; CDF4 ANTIGEN; FLUOROCHROME; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; AORTA WALL; ARTICLE; AUTOFLUORESCENCE; BLOOD; BLOOD VESSEL WALL; CELL DISRUPTION; CELL ISOLATION; CELL POPULATION; CELL SUSPENSION; CONTROLLED STUDY; ENZYMATIC DEGRADATION; FLOW CYTOMETRY; HYPERCHOLESTEROLEMIA; HYPERTENSION; INCUBATION TIME; KIDNEY; LIGHT SCATTERING; MACROPHAGE; MALE; MOUSE; NONHUMAN; POLARIZATION; PRIORITY JOURNAL; SINGLE CELL ANALYSIS; SPLEEN; THORACIC AORTA; THYMUS;

AORTA; AUTOFLUORESCENCE; FLOW CYTOMETRY; HYPERCHOLESTEROLEMI; HYPERTENSION; MACROPHAGE;

ANGIOTENSIN II; ANIMALS; ANTIGENS, CD45; ANTIGENS, DIFFERENTIATION; AORTA; APOLIPOPROTEINS E; CELLS, CULTURED; FLOW CYTOMETRY; FLUORESCENT DYES; HYPERCHOLESTEROLEMIA; HYPERTENSION; MACROPHAGES; MALE; MICE; MICE, INBRED C57BL;

MUS;

EID: 84884355967     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.07.009     Document Type: Article

References (30)

1. Andersson-Engels S., Klinteberg C., Svanberg K., Svanberg S. In vivo fluorescence imaging for tissue diagnostics. Phys. Med. Biol. 1997, 42:815.
2. Bush E., Maeda N., Kuziel W.A., Dawson T.C., Wilcox J.N., DeLeon H., Taylor W.R. CC chemokine receptor 2 is required for macrophage infiltration and vascular hypertrophy in angiotensin II-induced hypertension. Hypertension 2000, 36:360.
3. Butcher M.J., Herre M., Ley K., Galkina E. Flow cytometry analysis of immune cells within murine aortas. J. Vis. Exp. 2011, 53:e2848.
4. Chan C.T., Moore J.P., Budzyn K., Guida E., Diep H., Vinh A., Jones E.S., Widdop R.E., Armitage J.A., Sakkal S., Ricardo S.D., Sobey C.G., Drummond G.R. Reversal of vascular macrophage accumulation and hypertension by a CCR2 antagonist in deoxycorticosterone/salt-treated mice. Hypertension 2012, 60:1207.
5. Clozel M., Kuhn H., Hefti F., Baumgartner H.R. Endothelial dysfunction and subendothelial monocyte macrophages in hypertension effect of angiotensin converting enzyme inhibition. Hypertension 1991, 18:132.
6. Galkina E., Kadl A., Sanders J., Varughese D., Sarembock I.J., Ley K. Lymphocyte recruitment into the aortic wall before and during development of atherosclerosis is partially L-selectin dependent. J. Exp. Med. 2006, 203:1273.
7. Georgakoudi I., Jacobson B.C., Müller M.G., Sheets E.E., Badizadegan K., Carr-Locke D.L., Crum C.P., Boone C.W., Dasari R.R., Van Dam J., Feld M.S. NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes. Cancer Res. 2002, 62:682.
8. Glass C.K., Witztum J.L. Atherosclerosis. The road ahead. Cell 2001, 104:503.
9. Gordon S., Taylor P.R. Monocyte and macrophage heterogeneity. Nat. Rev. Immunol. 2005, 5:953.
10. Gray D.H.D., Chidgey A.P., Boyd R.L. Analysis of thymic stromal cell populations using flow cytometry. J. Immunol. Methods 2002, 260:15.
11. Gray D.H.D., Fletcher A.L., Hammett M., Seach N., Ueno T., Young L.F., Barbuto J., Boyd R.L., Chidgey A.P. Unbiased analysis, enrichment and purification of thymic stromal cells. J. Immunol. Methods 2008, 329:56.
12. Guzik T.J., Hoch N.E., Brown K.A., McCann L.A., Rahman A., Dikalov S., Goronzy J., Weyand C., Harrison D.G. Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction. J. Exp. Med. 2007, 204:2449.
13. Hammond K., Cain W., van Driel I., Godfrey D. Three day neonatal thymectomy selectively depletes NK1.1+ T cells. Int. Immunol. 1998, 10:1491.
14. Harrison D.G., Guzik T.J., Lob H.E., Madhur M.S., Marvar P.J., Thabet S.R., Vinh A., Weyand C.M. Inflammation, immunity, and hypertension. Hypertension 2011, 57:132.
15. Harrison D.G., Marvar P.J., Titze J.M. Vascular inflammatory cells in hypertension. Front. Physiol. 2012, 3:128.
16. Hulspas R., O'Gorman M.R.G., Wood B.L., Gratama J.W., Sutherland D.R. Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry B Clin. Cytom. 2009, 76:355.
17. Hume D.A., Robinson A.P., MacPherson G.G., Gordon S. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J. Exp. Med. 1983, 158:1522.
18. Judkins C.P., Diep H., Broughton B.R.S., Mast A.E., Hooker E.U., Miller A.A., Selemidis S., Dusting G.J., Sobey C.G., Drummond G.R. Direct evidence of a role for Nox2 in superoxide production, reduced nitric oxide bioavailability, and early atherosclerotic plaque formation in ApoE-/- mice. Am. J. Physiol. Heart Circ. Physiol. 2010, 298:H24.
19. Le T.T., Cheng J.-X. Single-cell profiling reveals the origin of phenotypic variability in adipogenesis. PLoS One 2009, 4:e5189.
20. Lecoeur H., de Oliveira-Pinto L.M., Gougeon M.-L. Multiparametric flow cytometric analysis of biochemical and functional events associated with apoptosis and oncosis using the 7-aminoactinomycin D assay. J. Immunol. Methods 2002, 265:81.
21. Lee Y.-H., Chen S.-Y., Wiesner R.J., Huang Y.-F. Simple flow cytometric method used to assess lipid accumulation in fat cells. J. Lipid Res. 2004, 45:1162.
22. Ley K., Miller Y.I., Hedrick C.C. Monocyte and macrophage dynamics during atherogenesis. Arterioscler. Thromb. Vasc. Biol. 2011, 31:1506.
23. Libby P., Ridker P.M., Hansson G.K. Progress and challenges in translating the biology of atherosclerosis. Nature 2011, 473:317.
24. Mitchell A.J., Pradel L.C., Chasson L., Van Rooijen N., Grau G.E., Hunt N.H., Chimini G. Technical advance: autofluorescence as a tool for myeloid cell analysis. J. Leukoc. Biol. 2010, 88:597.
25. Monici M. Cell and tissue autofluorescence research and diagnostic applications. Biotechnol. Annu. Rev. 2005, 11:227.
26. Murray P.J., Wynn T.A. Protective and pathogenic functions of macrophage subsets. Nat. Rev. Immunol. 2011, 11:723.
27. Philpott N.J., Turner A.J., Scopes J., Westby M., Marsh J.C., Gordon-Smith E.C., Dalgleish A.G., Gibson F.M. The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques. Blood 1996, 87:2244.
28. Swirski F.K., Libby P., Aikawa E., Alcaide P., Luscinskas F.W., Weissleder R., Pittet M.J. Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata. J. Clin. Invest. 2007, 117:195.
29. Tinker D.H., Rucker R.B., Tappel A.L. Variation of elastin fluorescence with method of preparation: determination of the major fluorophore of fibrillar elastin. Connect. Tissue Res. 1983, 11:299.
30. Wenzel P., Knorr M., Kossmann S., Stratmann J., Hausding M., Schuhmacher S., Karbach S.H., Schwenk M., Yogev N., Schulz E., Oelze M., Grabbe S., Jonuleit H., Becker C., Daiber A., Waisman A., Münzel T. Lysozyme M-positive monocytes mediate angiotensin II-induced arterial hypertension and vascular dysfunction. Circulation 2011, 124:1370.