Activation and control of p53 tetramerization in individual living cells

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 38, 2013, Pages 15497-15501

  Gaglia, Giorgio ^a   Guan, Yinghua ^a,b   Shah, Jagesh V ^a,b   Lahav, Galit ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

FCS; Mathematical model; Systems biology

Indexed keywords

DNA; OLIGOMER; PROTEIN P53; TETRAMER;

ARTICLE; CELL FUNCTION; CHEMICAL REACTION; DNA DAMAGE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; TETRAMERIZATION;

FCS; MATHEMATICAL MODEL; SYSTEMS BIOLOGY;

BLOTTING, WESTERN; CYCLOHEXIMIDE; DNA DAMAGE; GENE EXPRESSION REGULATION; HUMANS; MCF-7 CELLS; MODELS, BIOLOGICAL; POLYMERIZATION; SPECTROMETRY, FLUORESCENCE; TIME-LAPSE IMAGING; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84884296614     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1311126110     Document Type: Article

References (26)

1. Ali MH, Imperiali B (2005) Protein oligomerization: How and why. Bioorg Med Chem 13(17):5013-5020.
2. Funnell AP, Crossley M (2012) Homo-and heterodimerization in transcriptional regulation. Adv Exp Med Biol 747:105-121.
3. Kitayner M, et al. (2006) Structural basis of DNA recognition by p53 tetramers. Mol Cell 22(6):741-753.
4. Imagawa T, Terai T, Yamada Y, Kamada R, Sakaguchi K (2009) Evaluation of transcriptional activity of p53 in individual living mammalian cells. Anal Biochem 387(2): 249-256.
5. Kawaguchi T, et al. (2005) The relationship among p53 oligomer formation, structure and transcriptional activity using a comprehensive missense mutation library. Oncogene 24(46):6976-6981.
6. Davison TS, Yin P, Nie E, Kay C, Arrowsmith CH (1998) Characterization of the oligomerization defects of two p53 mutants found in families with Li-Fraumeni and Li-Fraumeni-like syndrome. Oncogene 17(5):651-656.
7. DiGiammarino EL, et al. (2002) A novel mechanism of tumorigenesis involving pHdependent destabilization of a mutant p53 tetramer. Nat Struct Biol 9(1):12-16.
8. Rajagopalan S, Huang F, Fersht AR (2011) Single-molecule characterization of oligomerization kinetics and equilibria of the tumor suppressor p53. Nucleic Acids Res 39(6):2294-2303.
9. Weinberg RL, Veprintsev DB, Fersht AR (2004) Cooperative binding of tetrameric p53 to DNA. J Mol Biol 341(5):1145-1159.
10. Sakaguchi K, et al. (1997) Phosphorylation of serine 392 stabilizes the tetramer formation of tumor suppressor protein p53. Biochemistry 36(33):10117-10124.
11. Rajagopalan S, Jaulent AM, Wells M, Veprintsev DB, Fersht AR (2008) 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers. Nucleic Acids Res 36(18):5983-5991.
12. Schumacher B, Mondry J, Thiel P, Weyand M, Ottmann C (2010) Structure of the p53 C-terminus bound to 14-3-3: Implications for stabilization of the p53 tetramer. FEBS Lett 584(8):1443-1448.
13. Ma L, et al. (2005) A plausible model for the digital response of p53 to DNA damage. Proc Natl Acad Sci USA 102(40):14266-14271.
14. Bode AM, Dong Z (2004) Post-translational modification of p53 in tumorigenesis. Nat Rev Cancer 4(10):793-805.
15. Weidemann T, Schwille P (2009) Fluorescence correlation spectroscopy in living cells. Handbook of Single-Molecule Biophysics, eds Hinterdorfer P, Oijen A (Springer, Dordrecht, The Netherlands), pp 217-241.
16. Macdonald P, Johnson J, Smith E, Chen Y, Mueller JD (2013) Brightness analysis. Methods Enzymol 518:71-98.
17. Brummelkamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296(5567):550-553.
18. Batchelor E, Mock CS, Bhan I, Loewer A, Lahav G (2008) Recurrent initiation: A mechanism for triggering p53 pulses in response to DNA damage. Mol Cell 30(3): 277-289.
19. Chen Y, Müller JD, So PT, Gratton E (1999) The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys J 77(1):553-567.
20. Brooks CL, Gu W (2011) p53 regulation by ubiquitin. FEBS Lett 585(18):2803-2809.
21. Fernandez-Fernandez MR, Veprintsev DB, Fersht AR (2005) Proteins of the S100 family regulate the oligomerization of p53 tumor suppressor. Proc Natl Acad Sci USA 102(13):4735-4740.
22. Saunders TE, et al. (2012) Noise reduction in the intracellular pom1p gradient by a dynamic clustering mechanism. Dev Cell 22(3):558-572.
23. Kremers GJ, Goedhart J, van Munster EB, Gadella TW, Jr. (2006) Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry 45(21):6570-6580.
24. Nagai T, et al. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20(1):87-90.
25. Cramer L, Mitchison TJ (1993) Moving and stationary actin filaments are involved in spreading of postmitotic PtK2 cells. J Cell Biol 122(4):833-843.
26. Kim SA, Heinze KG, Schwille P (2007) Fluorescence correlation spectroscopy in living cells. Nat Methods 4(11):963-973.