A novel nanoscopic tool by combining AFM with STED microscopy

Optical Nanoscopy

Volumn 1, Issue 1, 2012, Pages 1-6

  Harke, Benjamin ^a   Chacko, Jenu Varghese ^a,c   Haschke, Heiko ^b   Canale, Claudio ^a   Diaspro, Alberto ^a,c  

^a ISTITUTO ITALIANO DI TECNOLOGIA   (Italy)

^b JPK Instruments AG   (Germany)

^c UNIVERSITY OF GENOA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE IMAGING; STIMULATED EMISSION;

BIOLOGICAL SAMPLES; HIGH RESOLUTION; MECHANICAL IMAGING; STED MICROSCOPIES; STIMULATED EMISSION DEPLETION; SUPER RESOLUTION; TOPOGRAPHICAL IMAGING;

ATOMIC FORCE MICROSCOPY;

EID: 84884212782     PISSN: None     EISSN: 21922853     Source Type: Journal    
DOI: 10.1186/2192-2853-1-3     Document Type: Article

References (30)

1. Battaglia G, LoPresti C, Massignani M, et al.: Wet nanoscale imaging and testing of polymersomes. Small 2011, 7: 2010-2015.
2. Binnig G, Quate CF, Gerber C: Atomic force microscope. Phys Rev Lett 1986, 56: 930-933.
3. Butt HJ, Cappella B, Kappl M: Force measurements with the atomic force microscope: Technique, interpretation and applications. Surf Sci Rep 2005, 59: 1-152.
4. Dante S, Haufl T, Steitz R, et al.: Nanoscale structural and mechanical effects of beta-amyloid (1-42) on polymer cushioned membranes: A combined study by neutron reflectometry and AFM Force Spectroscopy. Biochimica et Biophysica Acta (BBA)-Biomembranes 2011, 1808: 2646-2655.
5. Domke J, Radmacher M: Measuring the elastic properties of thin polymer films with the atomic force microscope. Langmuir 1998, 14: 3320-3325.
6. Eggeling C, Ringemann C, Medda R, et al.: Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature 2008, 457: 1159-1162.
7. García-Sáez AJ, Schwille P: Surface analysis of membrane dynamics. Biochimica et Biophysica Acta (BBA) - Biomembranes 2010, 1798: 766-776.
8. Harke B: 3D STED microscopy with pulsed and continuous wave lasers. 2008. Nieders\ächsische Staats-und Universit\ätsbibliothek G\öttingen.
9. Harke B, Keller J, Ullal CK, et al.: Resolution scaling in STED microscopy. Opt Express 2008, 16: 4154-4162.
10. Hell SW: Toward fluorescence nanoscopy. Nat Biotechnol 2003, 21: 1347-1355.
11. Hell SW: Microscopy and its focal switch. Nat Methods 2009, 6: 24-32.
12. Hotta J, Fron E, Dedecker P, et al.: Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores. J Am Chem Soc 2010, 132: 5021-5023.
13. Kassies R, van Der Werf KO, Lenferink A, et al.: Combined AFM and confocal fluorescence microscope for applications in bio-nanotechnology. J Microsc 2005, 217: 109-116.
14. Le Grimellec C, Lesniewska E, Giocondi MC, et al.: Imaging of the surface of living cells by low-force contact-mode atomic force microscopy. Biophys J 1998, 75: 695-703.
15. Lenz MO, Brown ACN, Auksorius E, et al.: A STED-FLIM microscope applied to imaging the natural killer cell immune synapse. 2011, 79032D-79032D. -6.
16. Madl J, Rhode S, Stangl H, et al.: A combined optical and atomic force microscope for live cell investigations. Ultramicroscopy 2006, 106: 645-651.
17. Micic M, Hu D, Suh YD, et al.: Correlated atomic force microscopy and fluorescence lifetime imaging of live bacterial cells. Colloids Surf B Biointerfaces 2004, 34: 205-212.
18. Mueller V, Ringemann C, Honigmann A, et al.: STED Nanoscopy Reveals Molecular Details of Cholesterol-and Cytoskeleton-Modulated Lipid Interactions in Living Cells. Biophys J 2011, 101: 1651-1660.
19. Müller DJ, Dufrêne YF: Force nanoscopy of living cells. Curr Biol 2011, 21: R212-R216.
20. Nägerl UV, Willig KI, Hein B, et al.: Live-cell imaging of dendritic spines by STED microscopy. Proc Natl Acad Sci 2008, 105: 18982.
21. Pellett PA, Sun X, Gould TJ, et al.: Two-color STED microscopy in living cells. Biomed Opt Express 2011, 2: 2364-2371.
22. Schäpe J, Prausse S, Radmacher M, Stick R: Influence of lamin A on the mechanical properties of amphibian oocyte nuclei measured by atomic force microscopy. Biophys J 2009, 96: 4319-4325.
23. Schermelleh L, Heintzmann R, Leonhardt H: A guide to super-resolution fluorescence microscopy. J Cell Biol 2010, 190: 165-175.
24. Sharma S, Santiskulvong C, Bentolila LA, et al.: Correlative nanomechanical profiling with super-resolution F-actin imaging reveals novel insights into mechanisms of cisplatin resistance in ovarian cancer cells. Nanomedicine: Nanotechnology, Biology and Medicine.
25. Sieber JJ, Willig KI, Kutzner C, et al.: Anatomy and dynamics of a supramolecular membrane protein cluster. Science 2007, 317: 1072.
26. Stark M, Möller C, Müller DJ, Guckenberger R: From images to interactions: high-resolution phase imaging in tapping-mode atomic force microscopy. Biophys J 2001, 80: 3009-3018.
27. Urban NT, Willig KI, Hell SW, Nägerl UV: STED nanoscopy of actin dynamics in synapses deep inside living brain slices. Biophys J 2011, 101: 1277-1284.
28. Vickery SA, Dunn RC: Combining AFM and FRET for high resolution fluorescence microscopy. J Microsc 2001, 202: 408-412.
29. Westphal V, Rizzoli SO, Lauterbach MA, et al.: Video-rate far-field optical nanoscopy dissects synaptic vesicle movement. Science 2008, 320: 246.
30. Willig KI, Rizzoli SO, Westphal V, et al.: STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature 2006, 440: 935-939.