Blind assessment of localisation microscope image resolution

Optical Nanoscopy

Volumn 1, Issue 1, 2012, Pages 1-10

  Rees, Eric J ^a   Erdelyi, Miklos ^a,b   Pinotsi, Dorothea ^a   Knight, Alex ^b   Metcalf, Daniel ^b   Kaminski, Clemens F ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b NATIONAL PHYSICAL LABORATORY   (United Kingdom)

Author keywords

Density estimation; Localisation microscopy; Resolution; Super resolution

Indexed keywords

CRAMER-RAO BOUNDS; IMAGE RESOLUTION;

CALIBRATION SPECIMENS; DENSITY ESTIMATION; EMPIRICAL ESTIMATE; FLUORESCENT LABELS; LOCALISATION; MICROSCOPE IMAGES; RESOLUTION LIMITS; SUPER RESOLUTION;

OPTICAL RESOLVING POWER;

EID: 84884212024     PISSN: None     EISSN: 21922853     Source Type: Journal    
DOI: 10.1186/2192-2853-1-12     Document Type: Article

References (23)

1. Baddeley D, Cannell MB, Soeller C: Visualisation of localization microscopy data, Microsc. Microanal 2010, 16: 64-72.
2. Badieirostami M, Lew MD, Thompson MA, Moerner WE: Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane. Appl Phys Lett 2010, 97: 161103.
3. Bates M, Huang B, Dempsey GT, Zhuang X: Multicolor super-resolution imaging with photoswitchable fluorescent probes. Science 2007, 317: 1749-1753.
4. Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF: Imaging intracellular fluorescent proteins at nanometer resolution. Science 2006, 313: 1642-1645.
5. Breiman L, Meisel W, Purcell E: Variable kernel estimates of multivariate densities. Technometrics 1977, 19: 135-144.
6. Heilemann M, Linde S, Schuttpelz M, Kasper R, Seefeldt B, Mukherjee A, Tinnefeld P, Sauer M: Angew. Chem Int Ed 2008, 47: 6172-6176.
7. Hell SW: Far-field optical nanoscopy. Science 2007, 316: 1153-1158.
8. Hirschman II, Widder DV: The Convolution Transform. Princeton University Press, Princeton University Press, New Jersey; 1955.
9. Holtzman WH: The unbiased estimate of the population variance and standard deviation. Am J Psychol 1950, 63: 615-617.
10. Huang B, Wang WQ, Bates M, Zhuang X: Three-dimensional Super-resolution Imaging by Stochastic Optical Reconstruction Microscopy. Science 2008, 319: 810-813.
11. Juette MF, Gould TJ, Lessard MD, Mlodzianoski MJ, Nagpure BS, Bennett BT, Hess ST, Bewersdorf J: Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples. Nat Methods 2008, 5: 527-529.
12. Kaminski GS, Linde S, Erdelyi M, Esbjorner EK, Klein T, Rees E, Bertoncini CW, Dobson CM, Sauer M, Kaminski CF: In Situ Measurements of the Formation and Morphology of Intracellular β-Amyloid Fibrils by Super-Resolution Fluorescence Imaging. J Am Chem Soc 2011, 133: 12902-12905.
13. Kay SM: Fundamentals of Statistical Signal Processing: Estimation Theory, Prentice-Hall. 1993.
14. Ober RJ, Ram S, Ward ES: Localisation Accuracy in Single-Molecule Microscopy. Biophys J 2004, 86: 1185-1200.
15. Parzen E: On estimation of a probability density function and mode. Ann Math Stat 1962, 33: 1065-1076.
16. Schroff H, Galbriath CG, Galbriath JA, Betzig E: Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nat Methods 2008, 5: 417-423.
17. Shaevitz JW: Bayesian Estimation of the Axial Position in Astigmatism-Based Three-Dimensional Particle Tracking. International, Journal of Optics; 2009. 896208.
18. Shim SH, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, Wang X, Xu C, Bi GQ, Zhuang X: Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. PNAS 2012, 109: 13978-13983.
19. Silverman BW: Density estimation for statistics and data analysis, Chapman and Hall. 1986.
20. Sparrow CM: Astrophysical Journal. 1916, 44: 76-87.
21. Thompson RE, Larson DR, Webb WW: Precise nanometer localization analysis for individual fluorescent probes. Biophys J 2002, 82: 2775-2783.
22. Wolter S, Schuttpelz M, Tscherepanow M, Linde S, Heilemann M, Sauer M: Real-time computation of subdiffraction-resolution fluorescence images, J. Microscopy 2010, 237: 12-22.
23. Xu K, Babcock HP, Zhuang X: Dual-objective STORM reveals three-dimensional filament organisation in the actin cytoskeleton. Nat Methods 2012, 9: 185-188.