Cloning and Characterization of a Putative R2R3 MYB Transcriptional Repressor of the Rosmarinic Acid Biosynthetic Pathway from Salvia miltiorrhiza

PLoS ONE

Volumn 8, Issue 9, 2013, Pages

  Zhang, Shuncang ^a   Ma, Pengda ^a   Yang, Dongfeng ^b   Li, Wenjing ^a   Liang, Zongsuo ^a,b   Liu, Yan ^c   Liu, Fenghua ^c  

^a NORTHWEST A F UNIVERSITY   (China)

^b ZHEJIANG SCI TECH UNIVERSITY   (China)

^c TIANJIN UNIVERSITY OF TRADITIONAL CHINESE MEDICINE   (China)

Author keywords

[No Author keywords available]

Indexed keywords

4 HYDROXYLASE; OXYGENASE; PARA COUMARIC ACID; PHENOL; PLANT MEDICINAL PRODUCT; ROSMARINIC ACID; SALVIANOLIC ACID A; SALVIANOLIC ACID B; TYROSINE AMINOTRANSFERASE; UNCLASSIFIED DRUG;

4CL1 GENE; 4CL2 GENE; ACTIN GENE; ARTICLE; BIOSYNTHESIS; C4H GENE; CELL NUCLEUS; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; ENZYME SYNTHESIS; FLOWER; GENE FUNCTION; GENE LOCATION; GENE OVEREXPRESSION; GENE SEQUENCE; GENE SILENCING; HPPR GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PAL GENE; PHYLOGENY; PLANT GENE; PLANT LEAF; PLANT ROOT; PLANT STEM; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SALVIA MILTIORRHIZA; SEQUENCE ANALYSIS; SMMYB39 GENE; TAT GENE; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOSYNTHETIC PATHWAYS; CINNAMATES; CLONING, ORGANISM; DEPSIDES; GENE EXPRESSION REGULATION, PLANT; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; SALVIA MILTIORRHIZA; TRANSCRIPTION FACTORS;

EID: 84883830126     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0073259     Document Type: Article

References (54)

1. Xu H, Zhang L, Zhou CC, Xiao JB, Liao P, et al. (2010) Metabolic regulation and genetic engineering of pharmaceutical component tanshinone biosynthesis in Salvia miltiorrhiza. J Med Plants Res 4: 2591-2597.
2. Wu JY, Shi M, (2008) Ultrahigh diterpenoid tanshinone production through repeated osmotic stress and elicitor stimulation in fed-batch culture of Salvia miltiorrhiza hairy roots. Appl Microbiol Biotechnol 78: 441-448.
3. Zhou LM, Zuo Z, Chow MSS, (2005) Danshen: An overview of its chemistry, pharmacology, pharmacokinetics, and clinical use. J Clin Pharmacol 45: 1345-1359.
4. Nizamutdinova IT, Lee GW, Son KH, Jeon SJ, Kang SS, et al. (2008) Tanshinone I effectively induces apoptosis in estrogen receptor-positive (MCF-7) and estrogen receptor-negative (MDA-MB-231) breast cancer cells. Int J Oncol 33: 485-491.
5. Song YH, Liu Q, Lv ZP, Chen YY, Zhou YC, et al. (2008) Protection of a polysaccharide from Salvia miltiorrhiza, a Chinese medicinal herb, against immunological liver injury in mice. Int J Biol Macromol 43: 170-175.
6. Wan JMF, Sit WH, Lee CL, Fu KHM, Chan DKO, (2006) Protection of lethal toxicity of endotoxin by Salvia miltiorrhiza BUNGE is via reduction in tumor necrosis factor alpha release and liver injury. Int Immunopharmacol 6: 750-758.
7. Chen H, Chen F, Chiu FCK, Lo CMY, (2001) The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza. Enzyme Microb Technol 28: 100-105.
8. Li LN, (1998) Biologically active components from traditionaI Chinese medicines. Pure Appl Chem 70: 547-554.
9. Liu AH, Li L, Xu M, Lin YH, Guo HZ, et al. (2006) Simultaneous quantification of six major phenolic acids in the roots of Salvia miltiorrhiza and four related traditional Chinese medicinal preparations by HPLC-DAD method. J Pharm Biomed Anal 41: 48-56.
10. Petersen M, Simmonds MSJ, (2003) Molecules of interest- Rosmarinic acid. Phytochemistry 62: 121-125.
11. Don MJ, Shen CC, Syu WJ, Ding YH, Sun CM, (2006) Cytotoxic and aromatic constituents from Salvia miltiorrhiza. Phytochemistry 67: 497-503.
12. Shi Z, He J, Yao T, Chang W, Zhao M, (2005) Simultaneous determination of cryptotanshinone, tanshinone I and tanshinone IIA in traditional Chinese medicinal preparations containing Radix salvia miltiorrhiza by HPLC. J Pharm Biomed Anal 37: 481-486.
13. Petersen M, Häusler E, Karwatzki B, Meinhard J, (1993) Proposed biosynthetic pathway for rosmarinic acid in cell cultures of Coleus blumei Benth. Planta 189: 10-14.
14. Ellis B, Towers G, (1970) Biogenesis of rosmarinic acid in Mentha. Biochem J 118: 291.
15. China Pharmacopoeia Committee (2010) Chinese Pharmacopoeia of People's Republic of China. Beijing: Chemical Industry. 71.
16. Xiao Y, Gao SH, Di P, Chen JF, Chen WS, et al. (2010) Lithospermic acid B is more responsive to silver ions (Ag+) than rosmarinic acid in Salvia miltiorrhiza hairy root cultures. Biosci Rep 30: 33-40.
17. Petersen M, Abdullah Y, Benner J, Eberle D, Gehlen K, et al. (2009) Evolution of rosmarinic acid biosynthesis. Phytochemistry 70: 1663-1679.
18. Whetten R, Sederoff R, (1995) Lignin biosynthesis. Plant Cell 7: 1001-1013.
19. Feller A, Machemer K, Braun EL, Grotewold E, (2011) Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. Plant J 66: 94-116.
20. Katiyar A, Smita S, Lenka SK, Rajwanshi R, Chinnusamy V, et al. (2012) Genome-wide classification and expression analysis of MYB transcription factor families in rice and Arabidopsis. BMC Genomics 13: 544.
21. Jin HL, Martin C, (1999) Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol 41: 577-585.
22. Stracke R, Werber M, Weisshaar B, (2001) The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol 4: 447-456.
23. Kranz HD, Denekamp M, Greco R, Jin H, Leyva A, et al. (1998) Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J 16: 263-276.
24. Tamagnone L, Merida A, Parr A, Mackay S, Culianez-Macia FA, et al. (1998) The AmMYB308 and AmMYB330 transcription factors from Antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco. Plant Cell 10: 135-154.
25. Jin HL, Cominelli E, Bailey P, Parr A, Mehrtens F, et al. (2000) Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J 19: 6150-6161.
26. Fornale S, Sonbol FM, Maes T, Capellades M, Puigdomenech P, et al. (2006) Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyl-transferase genes by two new maize R2R3-MYB transcription factors. Plant Mol Biol 62: 809-823.
27. Fornale S, Shi XH, Chai CL, Encina A, Irar S, et al. (2010) ZmMYB31 directly represses maize lignin genes and redirects the phenylpropanoid metabolic flux. Plant J 64: 633-644.
28. Aharoni A, De Vos CHR, Wein M, Sun ZK, Greco R, et al. (2001) The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J 28: 319-332.
29. Gao JJ, Shen XF, Zhang Z, Peng RH, Xiong AS, et al. (2011) The myb transcription factor MdMYB6 suppresses anthocyanin biosynthesis in transgenic Arabidopsis. Plant Cell Tissue Organ Cult 106: 235-242.
30. Yan YP, Wang ZZ, (2007) Genetic transformation of the medicinal plant Salvia miltiorrhiza by Agrobacterium tumefaciens-mediated method. Plant Cell Tissue Organ Cult 88: 175-184.
31. Murashige T, Skoog F, (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant 15: 473-497.
32. Arnold K, Bordoli L, Kopp J, Schwede T, (2006) The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22: 195-201.
33. Schwede T, Kopp J, Guex N, Peitsch MC, (2003) SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res 31: 3381-3385.
34. Guex N, Peitsch MC, (1997) SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling. Electrophoresis 18: 2714-2723.
35. Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, et al. (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36: W465-W469.
36. Liu XY, Rodermel SR, Yu F, (2010) A var2 leaf variegation suppressor locus, SUPPRESSOR OF VARIEGATION3, encodes a putative chloroplast translation elongation factor that is important for chloroplast development in the cold. BMC Plant Biol 10: 287.
37. Yu F, Park S, Rodermel SR, (2004) The Arabidopsis FtsH metalloprotease gene family: interchangeability of subunits in chloroplast oligomeric complexes. Plant J 37: 864-876.
38. Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, et al. (2001) Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J 27: 581-590.
39. Gleave AP, (1992) A versatile binary vector system with a T-DNA organizational-structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20: 1203-1207.
40. Song J, Wang ZZ, (2011) RNAi-mediated suppression of the phenylalanine ammonia-lyase gene in Salvia miltiorrhiza causes abnormal phenotypes and a reduction in rosmarinic acid biosynthesis. J Plant Res 124: 183-192.
41. Liang ZS, Yang DF, Liang X, Zhang YJ, Liu Y, et al. (2012) Roles of reactive oxygen species in methyl jasmonate and nitric oxide-induced tanshinone production in Salvia miltiorrhiza hairy roots. Plant Cell Rep 31: 873-883.
42. Yan Q, Shi M, Ng J, Wu JY, (2006) Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots. Plant Sci 170: 853-858.
43. Lamb Cj, Rubery PH, (1975) A spectrophotometric assay for Trans-cinnamic acid 4-Hydroxylase activity. Anal Biochem 68: 554-561.
44. Shen H, He XZ, Poovaiah CR, Wuddineh WA, Ma JY, et al. (2012) Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks. New Phytol 193: 121-136.
45. Legay S, Lacombe E, Goicoechea M, Briere C, Seguin A, et al. (2007) Molecular characterization of EgMYB1, a putative transcriptional repressor of the lignin biosynthetic pathway. Plant Sci 173: 542-549.
46. Colquhoun TA, Kim JY, Wedde AE, Levin LA, Schmitt KC, et al. (2011) PhMYB4 fine-tunes the floral volatile signature of Petunia × hybrida through PhC4H. J Exp Bot 62: 1133-1143.
47. Zhang YA, Yan YP, Wang ZZ, (2010) The Arabidopsis PAP1 transcription factor plays an important role in the enrichment of phenolic acids in Salvia miltiorrhiza. J Agric Food Chem 58: 12168-12175.
48. Laitinen RAE, Ainasoja M, Broholm SK, Teeri TH, Elomaa P, (2008) Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida. J Exp Bot 59: 3691-3703.
49. Russell DW, (1971) The metabolism of aromatic compounds in higher plants. J Biol Chem 246: 3870-3878.
50. Carra A, Schubert A, Martin C (2005) Isolation and preliminary characterization of VvMYB3, a novel MYB transcription factor from grape. In: Williams LE, Proceedings of the Seventh International Symposium on Grapevine Physiology and Biotechnology. 529-531.
51. Sandorf I, Hollander-Czytko H, (2002) Jasmonate is involved in the induction of tyrosine aminotransferase and tocopherol biosynthesis in Arabidopsis thaliana. Planta 216: 173-179.
52. Douce R, Joyard J (1996) Biosynthesis of thylakoid membrane lipids. In: Ort DR, Yocum CF, Advances in photosynthesis/oxygenic photosynthesis: the light reactions Dordrecht: Kluwer. 69-101.
53. Liu LS, White MJ, MacRae TH, (1999) Transcription factors and their genes in higher plants- Functional domains, evolution and regulation. Eur J Biochem 262: 247-257.
54. Zhao JF, Zhang WH, Zhao Y, Gong XM, Guo L, et al. (2007) SAD2, an importin beta-like protein, is required for UV-B response in Arabidopsis by mediating MYB4 nuclear trafficking. Plant Cell 19: 3805-3818.