Rapid and sensitive HILIC-MS/MS analysis of carnitine and acetylcarnitine in biological fluids

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 23, 2013, Pages 7397-7404

  Isaguirre, Andrea C ^a   Olsina, Roberto A ^a   Martinez, Luis D ^a   Lapierre, Alicia V ^a,b   Cerutti, Soledad ^a  

^a Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

^b UNIVERSIDAD NACIONAL DE SAN LUIS   (Argentina)

Author keywords

Acetylcarnitine; Biological fluids; Carnitine; Hydrophilic interaction liquid chromatography tandem mass spectrometry

Indexed keywords

ACETYLCARNITINE; ANALYTICAL APPROACH; BIOLOGICAL FLUIDS; CARNITINE; HYDROPHILIC INTERACTION; LIMITS OF DETECTION; LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; RELATED COMPOUNDS;

CHROMATOGRAPHY; MASS SPECTROMETRY; RNA;

BODY FLUIDS;

ACETYLCARNITINE; CARNITINE;

ADULT; ARTICLE; BLOOD; CHEMICAL PHENOMENA; FEMALE; HUMAN; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY; MALE; MIDDLE AGED; TANDEM MASS SPECTROMETRY; URINE;

ACETYLCARNITINE; ADULT; CARNITINE; CHROMATOGRAPHY, LIQUID; FEMALE; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LIMIT OF DETECTION; MALE; MIDDLE AGED; TANDEM MASS SPECTROMETRY;

EID: 84883560943     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-013-7193-6     Document Type: Article

References (41)

1. Rebouche CJ (1992) Carnitine function and requirements during the life cycle. FASEB J 6:3379-3386
2. 1H-NMR spectroscopy. Anal Bioanal Chem 399:2285-2294
3. Reuter SE, Evans AM (2012) Carnitine and acylcarnitines: pharmacokinetic, pharmacological and clinical aspects. Clin Pharmacokinet 9:553-572
4. Huang A, Owen K (2013) Role of supplementary L-carnitine in exercise and exercise recovery. Curr Asp Recover 59:135-142
5. Mels CMC, Schute AE, Erasmus E, Huisman HW, Shute R, Fourie CMT, Kruger R, Van Rooyen JM, Smith W, Malan NT, Malan L (2013) l-Carnitine and long-chain acylcarnitines are positively correlated with ambulatory blood pressure in humans: the SABPA Study. Lipids 48:63-73
6. Christie WW (2012) Carnitine and acylcarnitines. Structure, occurrence, biology and analysis. The AOCS Lipid Library. http://lipidlibrary.aocs.org/ lipids/carnitin/index.htm. Accessed 10 Dec 2012
7. Dunning KR, Robker RL (2012) Promoting lipid utilization with l-carnitine to improve oocyte quality. Anim Reprod Sci 134:69-75
8. Costa CG, Struys EA, Bootsma A, ten Brink HJ, Dorland L, Tavares de Almeida I, Duran M, Jakobs C (1997) Quantitative analysis of plasma acylcarnitines using gas chromatography chemical ionization mass fragmentography. J Lipid Res 38:173-182
9. Benvenga S (2005) Effects of L-carnitine on thyroid hormone metabolism and on physical exercise tolerance. Horm Metab Res 37:566-571
10. Noland RC, Koves TR, Seller RD, Lum H, Lust RM, Ilkayeva O, Stevens RD, Hegardt FG, Muolo DM (2009) Carnitine insufficiency caused by aging and overnutrition compromises mitochondrial performance and metabolic control. J Biol Chem 284:22840-22852
11. Sinclair C, Gilchrist JM, Hennessey JV, Kandula M (2005) Muscle carnitine in hypo- and hyperthyroidism. Muscle Nerve 32:357-359
12. Mir S, Kantar M, Yalaz M, Keskinoglu A, Coker I, Huseyinov A (2002) Effect of hemodialysis on carnitine levels in children with chronic failure. Pediatr Int 44:70-73
13. Vieira Neto E, Fonseca AA, Almeida RF, Figueiredo MP, Porto MAS, Ribeiro MG (2012) Analysis of acylcarnitine profiles in umbilical cord blood and during the early neonatal period by electrospray ionization tandem mass spectrometry. Braz J Med Biol Res 45:473-564
14. Desiderio C, De Ross A, Inzitari R, Mancinelli A, Rossetti DV, Castagnola M, Messana I (2008) Optimization of a rapid capillary electrophoresis ESI-IT tandem mass spectrometry method for the analysis of short-chain carnitines in human plasma. Anal Bioanal Chem 390:1637-1644
15. Stevens RD, Hillman SL, Worthy S, Sanders D, Millington DS (2000) Assay for free and total carnitine in human plasma using tandem mass spectrometry. Clin Chem 46:727-729
16. Moder M, Kießling A, Löster H, Brüggeman L (2003) The pattern of urinary acylcarnitines determined by electrospray mass spectrometry: a new tool in the diagnosis of diabetes mellitus. Anal Bioanal Chem 375:200-210
17. Fahmy SF, El-hamamsy MH, Zaki OK, Badary OA (2013) l-Carnitine supplementation improves the behavioral symptoms in autistic children. Res Autism Spectr Disord 7:159-166
18. Moder M, Löster H, Herzschuh R, Popp P (1997) Determination of urinary acylcarnitines by ESI-MS coupled with solid-phase microextraction (SPME). J Mass Spectrom 32:1195-1204
19. Vernez L, Wenk M, Krähenbühl S (2004) Determination of carnitine and acylcarnitines in plasma by high-performance liquid chromatography electrospray ionization ion trap tandem mass spectrometry. Rapid Commun Mass Spectrom 18:1233-1238
20. Falasca S, Petruzziello F, Kretz R, Rainer G, Zhang X (2012) Analysis of multiple quaternary ammonium compounds in the brain using tandem capillary column separation and high resolution mass spectrometric detection. J Chromatogr A 1241:46-51
21. Pozo OJ, Sancho JV, Ivañez M, Hernández F, Nielssen WMA (2006) Confirmation of organic micropollutants detected in environmental samples by liquid chromatography tandem mass spectrometry: achievements and pitfalls. Trends Anal Chem 25:1030-1042
22. Chen J, Wang W, Lu S, Yin P, Zhao X, Lu X, Zhang F, Xu G (2009) Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations. Anal Chim Acta 650:3-9
23. Gucciardi A, Pirillo P, Di Gangi IM, Naturale M, Giordano G (2012) A rapid UPLC-MS/MS method for simultaneous separation of 48 acylcarnitines in dried blood spots and plasma useful as a second-tier test for expanded newborn screening. Anal Bioanal Chem 404:741-751
24. Sowell J, Fuqua M, Wood T (2011) Quantification of total and free carnitine in human plasma by hydrophilic interaction liquid chromatography tandem mass spectrometry. J Chromatogr Sci 49:463-468
25. Nguyen DT-T, Guillarme D, Rudaz S, Veuthey J-L (2006) Fast analysis in liquid chromatography using small particle size and high pressure. J Sep Sci 29:1836-1848
26. TM: an introduction and review. J Liq Chromatogr Relat Technol 28:1253-1263
27. Miller JH IV, Poston PA, Karnes HT (2012) A quantitative method for acylcarnitines and amino acids using high resolution chromatography and tandem mass spectrometry in newborn screening dried blood spot analysis. J Chromatogr B 903:142-149
28. Hemstrom P, Irgum K (2006) Hydrophilic interaction chromatography. J Sep Sci 29:1784-1821
29. Grumbach ES, Wagrowski-Diehl DM, Mazzeo JR, Alden B, Iraneta PC (2004) Hydrophilic interaction chromatography using silica columns for the retention of polar analytes and enhanced ESI-MS sensitivity. LCGC N Am 22:1010-1023
30. Alpert AJ (1990) Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds. J Chromatogr 499:176-196
31. Zhi L, Mutlib AE, Wang J, Talaat RE (2008) Liquid chromatography/mass spectrometry determination of endogenous plasma acetyl and palmitoyl carnitines as potential biomarkers of β-oxidation in mice. Rapid Commun Mass Spectrom 22:3434-3442
32. 1HNMR and HPLC MS. Mol Biosyst 1:166-175
33. Ghoshal AK, Guo T, Soukhova N, Soldin SJ (2005) Rapid measurement of plasma acylcarnitines by liquid chromatography-tandem mass spectrometry without derivatization. Clin Chim Acta 358:104-112
34. Tallarico C, Pace S, Longo A (1998) Quantitation of L-carnitine, acetyl-L-carnitine, propionyl-L-carnitine and their deuterated analogues by high-performance liquid chromatography tandem mass spectrometry. Rapid Commun Mass Spectrom 12:403-409
35. Hirche F, Fischer M, Keller J, Eder K (2009) Determination of carnitine, its short chain acyl esters and metabolic precursors trimethyllysine and γ-butyrobetaine by quasi-solid phase extraction and MS/MS detection. J Chromatogr B 877:2158-2162
36. Constantopoulos TL, Jacksson GS, Enke CG (1999) Effects of salt concentration on analyte response using Electrospray ionization mass spectrometry. J Am Soc Mass Spectrom 10:625-634
37. Apffel A, Fischer S, Goldberg G, Kuhlmann FE (1995) Enhanced sensitivity for peptide mapping with electrospray liquid chromatography-mass spectrometry in the presence of signal suppression due to trifluoroacetic acid-containing mobile phase. J Chromatogr A 712:177-190
38. Steen RJ, Leonards PEG, Brinkman UAT (1999) Environ Toxicol Chem 18:1574-1581
39. Heaton J, Gray N, Cowan DA, Plumb RS, Legido-Quigley C, Smith NW (2012) Comparison of reversed-phase and hydrophilic interaction liquid chromatography for the separation of ephedrines. J Chromatogr A 1228:329-337
40. Ruta J, Rudaz S, McCalley DV, Veuthey JL, Guillarme D (2010) A systematic investigation of the effect of sample diluents of peak shape in hydrophilic interaction liquid chromatography. J Chromatogr A 1217:8230-8240
41. Broad E, Bolger C, Galloway S (2006) Dietary carnitine intake and carnitine status in endurance-trained males. Nutr Diet 63:148-154