Pseudo attP sites in favor of transgene integration and expression in cultured porcine cells identified by streptomyces phage phiC31 integrase

BMC Molecular Biology

Volumn 14, Issue , 2013, Pages

  Bi, Yanzhen ^a   Liu, Ximei ^a   Zhang, Long ^a,b   Shao, Changwei ^c   Ma, Zhuo ^a,d   Hua, Zaidong ^a   Zhang, Liping ^a   Li, Li ^a   Hua, Wenjun ^a   Xiao, Hongwei ^a   Wei, Qingxin ^a   Zheng, Xinmin ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b YANGTZE UNIVERSITY   (China)

^c WUHAN UNIVERSITY   (China)

^d NORTHEAST AGRICULTURAL UNIVERSITY   (China)

Author keywords

PhiC31 integrase; Pig; Pseudo attP site; TAIL PCR

Indexed keywords

EPIDERMAL GROWTH FACTOR; INTEGRASE; PHIC31 PROTEIN; PLASMID DNA; UNCLASSIFIED DRUG;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ALLELE; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BACTERIOPHAGE; CELL CLONE; CELL PROLIFERATION; CONTROLLED STUDY; DNA INTEGRATION; DOUBLE STRANDED DNA BREAK; GENE DELETION; GENE EXPRESSION; GENE INSERTION; GENE LOCATION; GENE SEQUENCE; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETIC SELECTION; GENETIC TRANSFECTION; GENOME; KIDNEY CELL; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; POSITION EFFECT; PROTEIN EXPRESSION; STREPTOMYCES; TRANSGENE;

EUKARYOTA; STREPTOMYCES; SUIDAE; SUS;

ANIMALS; ATTACHMENT SITES, MICROBIOLOGICAL; BASE SEQUENCE; CELL LINE; CELL PROLIFERATION; DNA BREAKS, DOUBLE-STRANDED; GENOME; INTEGRASES; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; SIPHOVIRIDAE; STREPTOMYCES; SWINE; TRANSFECTION; TRANSGENES;

EID: 84883508761     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-14-20     Document Type: Article

References (30)

1. Gama Sosa MA, De Gasperi R, Elder GA. Animal transgenesis: an overview. Brain Struct Funct 2010, 214:91-109. 10.1007/s00429-009-0230-8, 19937345.
2. Wilson MH, Coates CJ, George AL. PiggyBac transposon-mediated gene transfer in human cells. Mol Ther 2007, 15:139-145. 10.1038/sj.mt.6300028, 17164785.
3. Hoffmann A, Kessler B, Ewerling S, Weppert M, Vogg B, Ludwig H, Stojkovic M, Boelhauve M, Brem G, Wolf E, Pfeifer A. Efficient transgenesis in farm animals by lentiviral vectors. EMBO Rep 2003, 4:1054-1060. 10.1038/sj.embor.7400007, 1326377, 14566324.
4. Jakobsen JE, Li J, Kragh PM, Moldt B, Lin L, Liu Y, Schmidt M, Winther KD, Schyth BD, Holm IE, Vajta G, Bolund L, Callesen H, Jørgensen AL, Nielsen AL, Mikkelsen JG. Pig transgenesis by Sleeping Beauty DNA transposition. Transgenic Res 2011, 20:533-545. 10.1007/s11248-010-9438-x, 20803249.
5. Carlson DF, Garbe JR, Tan W, Martin MJ, Dobrinsky JR, Hackett PB, Clark KJ, Fahrenkrug SC. Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system. Transgenic Res 2011, 20:1125-1137. 10.1007/s11248-010-9481-7, 21221779.
6. Garrels W, Mátés L, Holler S, Dalda A, Taylor U, Petersen B, Niemann H, Izsvák Z, Ivics Z, Kues WA. Germline transgenic pigs by sleeping beauty transposition in porcine zygotes and targeted integration in the Pig genome. PLoS ONE 2011, 6:1-12.
7. Williams A, Harker N, Ktistaki E, Veiga-Fernandes H, Roderick K, Tolaini M, Norton T, Williams K, Kioussis D. Position effect variegation and imprinting of transgenes in lymphocytes. Nucleic Acids Res 2008, 36:2320-2329. 10.1093/nar/gkn085, 2367730, 18296483.
8. Friedel RH, Plump A, Lu X, Spilker K, Jolicoeur C, Wong K, Venkatesh TR, Yaron A, Hynes M, Chen B. Gene targeting using a promoterless gene trap vector (" targeted trapping" ) is an efficient method to mutate a large fraction of genes. Proc Natl Acad Sci USA 2005, 102:13188-13193. 10.1073/pnas.0505474102, 1193537, 16129827.
9. Cui X, Ji D, Fisher DA, Wu Y, Briner DM, Weinstein EJ. Targeted integration in rat and mouse embryos with zinc-finger nucleases. Nat Biotechnol 2011, 29:64-67. 10.1038/nbt.1731, 21151125.
10. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 2013, 152:1173-1183. 10.1016/j.cell.2013.02.022, 3664290, 23452860.
11. Thorpe HM, Smith M. In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc Natl Acad Sci USA 1998, 95:5505-5510. 10.1073/pnas.95.10.5505, 20407, 9576912.
12. Groth AC, Fish M, Nusse R, Calos MP. Construction of transgenic Drosophila by using the site-specific integrase from phage PhiC31. Genetics 2004, 166:1775-1782. 10.1534/genetics.166.4.1775, 1470814, 15126397.
13. Nakayama G, Kawaguchi Y, Koga K, Kusakabe T. Site-specific gene integration in cultured silkworm cells mediated by PhiC31 integrase. Mol Genet Genomics 2006, 275:1-8. 10.1007/s00438-005-0026-3, 16341709.
14. Olivares EC, Hollis RP, Chalberg TW, Meuse L, Kay MA, Calos MP. Site-specific genomic integration produces therapeutic Factor IX levels in mice. Nat Biotechnol 2002, 20:1124-1128. 10.1038/nbt753, 12379870.
15. Chalberg TW, Genise HL, Vollrath D, Calos MP. PhiC31 integrase confers genomic integration and long-term transgene expression in rat retina. Invest Ophthalmol Vis Sci 2005, 46:2140-2146. 10.1167/iovs.04-1252, 15914635.
16. Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L. Site-specific integrase-mediated transgenesis in mice via pronuclear injection. Proc Natl Acad Sci 2011, 108:7902-7907. 10.1073/pnas.1019507108, 3093482, 21464299.
17. Ma QW, Sheng HQ, Yan JB, Cheng S, Huang Y, Chen-Tsai Y, Ren ZR, Huang SZ, Zeng YT. Identification of pseudo attP sites for phage PhiC31 integrase in bovine genome. Biochem Biophys Res Commun 2006, 345:984-988. 10.1016/j.bbrc.2006.04.145, 16712792.
18. Ou HL, Huang Y, Qu LJ, Xu M, Yan JB, Ren ZR, Huang SZ, Zeng YT. A PhiC31 integrase-mediated integration hotspot in favor of transgene expression exists in the bovine genome. FEBS Journal 2009, 276:155-163. 10.1111/j.1742-4658.2008.06762.x, 19019083.
19. Yu Y, Wang Y, Tong Q, Liu X, Su F, Quan F, Guo Z, Zhang Y. A site-specific recombinase-based method to produce antibiotic selectable marker free transgenic cattle. PLOS One 2013, 8:1-14.
20. Liu Y, Mitsukawa N, Oosumi T, Whittier RF. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 1995, 8:457-463. 10.1046/j.1365-313X.1995.08030457.x, 7550382.
21. Ehrhardt A, Xu H, Huang Z, Engler JA, Kay MA. A direct comparison of two nonviral gene therapy vectors for somatic integration: in vivo evaluation of the bacteriophage integrase PhiC31 and the sleeping beauty transposase. Mol Ther 2005, 11:695-706. 10.1016/j.ymthe.2005.01.010, 15851008.
22. Bi Y, Qiao X, Hua Z, Zhang L, Liu X, Li L, Hua W, Xiao H, Zhou J, Wei Q. An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy. BMC Biotechnol 2012, 12:39-50. 10.1186/1472-6750-12-39, 3408372, 22768962.
23. Qu L, Ma Q, Zhou Z, Ma H, Huang Y, Huang S, Zeng F, Zeng Y. A profile of native integration sites used by PhiC31 integrase in the bovine genome. J Genet Genomics 2012, 39:217-224. 10.1016/j.jgg.2012.03.004, 22624883.
24. Wu X, Li Y, Crise B, Burgess SM. Transcription start regions in the human genome are favored targets for MLV integration. Science 2003, 300:1749-1751. 10.1126/science.1083413, 12805549.
25. Garrels W, Ivics Z, Kues WA. Precision genetic engineering in large mammals. Trends Biotechnol 2012, 30:386-393. 10.1016/j.tibtech.2012.03.008, 22521716.
26. Tan WS, Carlson DF, Walton MW, Fahrenkrug SC, Hackett PB. Precision editing of large animal genomes. Adv Genet 2012, 80:37-97. 3683964, 23084873.
27. Wang B, Xu G, Zhou C, Tian L, Xue J, Chen J, Jia W. ΦC31 integrase interacts with TTRAP and inhibits NFκB activation. Mol Biol Rep 2010, 37:2809-2816. 10.1007/s11033-009-9829-3, 19757154.
28. Liu S, Ma J, Wang W, Zhang M, Xin Q, Peng S, Li R, Zhu H. Mutational analysis of highly conserved residues in the phage phiC31 integrase reveals key amino acids necessary for the DNA recombination. PLoS One 2010, 5:e8863. 10.1371/journal.pone.0008863, 2810336, 20111606.
29. Bi Y, Zhang J, Ma J, Ouyang H, Zheng X, Qiao X. One-stop polymerase chain reaction (PCR): An improved PCR method with speedy operation and comparable efficiency. Af J Biotechnol 2011, 10:19494-19498.
30. Guichard PP, Bi Y, Cayrol B, Marco S, Arluison V. Involvement of HFq protein in the post-transcriptional regulation of E. coli bacterial cytoskeleton and cell division proteins. Cell Cycle 2009, 8:2470-2472. 10.4161/cc.8.15.9090, 19571680.