Development of a novel ex vivo porcine skin explant model for the assessment of mature bacterial biofilms

Wound Repair and Regeneration

Volumn 21, Issue 5, 2013, Pages 704-714

  Yang, Qingping ^a   Phillips, Priscilla L ^a   Sampson, Edith M ^a   Progulske Fox, Ann ^a   Jin, Shouguang ^a   Antonelli, Patrick ^a   Schultz, Gregory S ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORINE; GENTAMICIN; HYPOCHLORITE SODIUM; OXACILLIN;

ANIMAL TISSUE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL LOAD; BIOFILM; CELL ENGINEERING; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; DERMIS; EPIDERMIS; EX VIVO STUDY; EXPLANT; HISTOLOGY; INSTRUMENT STERILIZATION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PARTIAL THICKNESS WOUND; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; QUORUM SENSING; SCANNING ELECTRON MICROSCOPY; SKIN; SKIN EXPLANT; STAPHYLOCOCCUS AUREUS; SWINE; WILD TYPE; WOUND;

ANIMALS; ANTI-BACTERIAL AGENTS; BIOFILMS; EPIDERMIS; GENTAMICINS; MICROSCOPY, ELECTRON, SCANNING; OXACILLIN; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; REPRODUCIBILITY OF RESULTS; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; SWINE; WOUND HEALING; WOUND INFECTION;

EID: 84883486872     PISSN: 10671927     EISSN: 1524475X     Source Type: Journal    
DOI: 10.1111/wrr.12074     Document Type: Article

References (35)

1. Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, et al. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen 2009; 17: 763-771.
2. Armstrong DG, Lavery LA,. Clinical care of the diabetic foot. Alexandria, VA: American Diabetes Association, 2005.
3. Duncan KD,. Preventing pressure ulcers: the goal is zero. Jt Comm J Qual Patient Saf 2007; 33: 605-610.
4. James GA, Swogger E, Wolcott R, Pulcini E, Secor P, Sestrich J, et al. Biofilms in chronic wounds. Wound Repair Regenen 2008; 16: 37-44.
5. Jones SG, Edwards R, Thomas DW,. Inflammation and wound healing: the role of bacteria in the immuno-regulation of wound healing. Int J Low Extrem Wounds 2004; 3: 201-208.
6. Costerton JW, Stewart PS, Greenberg EP,. Bacterial biofilms: a common cause of persistent infections. Science 1999; 284: 1318-1322.
7. Davis SC, Ricotti C, Cazzaniga A, Welsh E, Eaglstein WH, Mertz PM,. Microscopic and physiologic evidence for biofilm-associated wound colonization in vivo. Wound Repair Regen 2008; 16: 23-29.
8. Donlan RM, Costerton JW,. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 2002; 15: 167-193.
9. Davies DG, Parsek MR, Pearson JP, Iglewski BH, Costerton JW, Greenberg EP,. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 1998; 280: 295-298.
10. O'Toole GA, Gibbs KA, Hager PW, Phibbs PV Jr, Kolter R,. The global carbon metabolism regulator Crc is a component of a signal transduction pathway required for biofilm development by Pseudomonas aeruginosa. J Bacteriol 2000; 182: 425-431.
11. Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM,. Microbial biofilms. Annu Rev Microbiol 1995; 49: 711-745.
12. Watnick P, Kolter R,. Biofilm, city of microbes. J Bacteriol 2000; 182: 2675-2679.
13. Stewart PS, Costerton JW,. Antibiotic resistance of bacteria in biofilms. Lancet 2001; 358: 135-138.
14. Stewart PS, Rayner J, Roe F, Rees WM,. Biofilm penetration and disinfection efficacy of alkaline hypochlorite and chlorosulfamates. J Appl Microbiol 2001; 91: 525-532.
15. Edwards R, Harding KG,. Bacteria and wound healing. Curr Opin Infect Dis 2004; 17: 91-96.
16. Gurjala AN, Geringer MR, Seth AK, Hong SJ, Smeltzer MS, Galiano RD, et al. Development of a novel, highly quantitative in vivo model for the study of biofilm-impaired cutaneous wound healing. Wound Repair Regen 2011; 19: 400-410.
17. Ren D, Bedzyk LA, Thomas SM, Ye RW, Wood TK,. Gene expression in Escherichia coli biofilms. Appl Microbiol Biotechnol 2004; 64: 515-524.
18. Cho KH, Caparon MG,. Patterns of virulence gene expression differ between biofilm and tissue communities of Streptococcus pyogenes. Mol Microbiol 2005; 57: 1545-1556.
19. Coenye T, Nelis HJ,. In vitro and in vivo model systems to study microbial biofilm formation. J Microbiol Methods 2010; 83: 89-105.
20. Pearson JP, Pesci EC, Iglewski BH,. Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J Bacteriol 1997; 179: 5756-5767.
21. Pier GB, Boyer D, Preston M, Coleman FT, Llosa N, Mueschenborn-Koglin S, et al. Human monoclonal antibodies to Pseudomonas aeruginosa alginate that protect against infection by both mucoid and nonmucoid strains. J Immunol 2004; 173: 5671-5678.
22. Stewart PS, Franklin MJ,. Physiological heterogeneity in biofilms. Nat Rev Microbiol 2008; 6: 199-210.
23. Boulos L, Prevost M, Barbeau B, Coallier J, Desjardins R,. LIVE/DEAD BacLight: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. J Microbiol Methods 1999; 37: 77-86.
24. Schmook FP, Meingassner JG, Billich A,. Comparison of human skin or epidermis models with human and animal skin in in-vitro percutaneous absorption. Int J Pharm 2001; 215: 51-56.
25. Sullivan TP, Eaglstein WH, Davis SC, Mertz P,. The pig as a model for human wound healing. Wound Repair Regen 2001; 9: 66-76.
26. Rumbaugh KP, Griswold JA, Hamood AN,. Pseudomonas aeruginosa strains obtained from patients with tracheal, urinary tract and wound infection: variations in virulence factors and virulence genes. J Hosp Infect 1999; 43: 211-218.
27. Singh PK, Schaefer AL, Parsek MR, Moninger TO, Welsh MJ, Greenberg EP,. Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms. Nature 2000; 407: 762-764.
28. Stickler DJ, Clayton CL, Harber MJ, Chawla JC,. Pseudomonas aeruginosa and long-term indwelling bladder catheters. Arch Phys Med Rehabil 1988; 69: 25-28.
29. Siegel JD, Rhinehart E, Jackson M, Chiarello L,. 2007 guideline for isolation precautions: preventing transmission of infectious agents in health care settings. Am J Infect Control 2007; 35 (10 Suppl. 2): S65-164.
30. King M,. Our historical roots: Dr Richard Drysdale Dakin, DSc, and his solution. J Wound Ostomy Continence Nurs 2008; 35: 289-292.
31. Dequeiroz GA, Day DF,. Antimicrobial activity and effectiveness of a combination of sodium hypochlorite and hydrogen peroxide in killing and removing Pseudomonas aeruginosa biofilms from surfaces. J Appl Microbiol 2007; 103: 794-802.
32. Wirtanen G, Salo S, Helander IM, Mattila-Sandholm T,. Microbiological methods for testing disinfectant efficiency on Pseudomonas biofilm. Colloids Surf B Biointerfaces 2001; 20: 37-50.
33. Wolcott RD, Rumbaugh KP, James G, Schultz G, Phillips P, Yang Q, et al. Biofilm maturity studies indicate sharp debridement opens a time-dependent therapeutic window. J Wound Care 2010; 19: 320-328.
34. Southey-Pillig CJ, Davies DG, Sauer K,. Characterization of temporal protein production in Pseudomonas aeruginosa biofilms. J Bacteriol 2005; 187: 8114-8126.
35. Prigent-Combaret C, Vidal O, Dorel C, Lejeune P,. Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli. J Bacteriol 1999; 181: 5993-6002.